Q98N65 (RPOC_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	mlr0277

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	1398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1398	1398	DNA-directed RNA polymerase subunit beta' HAMAP MF_01322		PRO_0000067782

Sequences

	Sequence		Length	Mass (Da)
	Q98N65 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: D5DD7A297B62449A	FASTA	1,398	155,101
	10 20 30 40 50 60 MNQEVMNLFN PQAPAQVFDS IRISLASPEK ILSWSFGEIK KPETINYRTF KPERDGLFCA 70 80 90 100 110 120 RIFGPIKDYE CLCGKYKRMK YKGVICEKCG VEVTLSRVRR ERMGHIELAA PVAHIWFLKS 130 140 150 160 170 180 LPSRIGTLLD MTLKDIERVL YFENYIVTEP GLTALKEHQL LSEEEYMIAV DEYGEDSFTA 190 200 210 220 230 240 MIGAEAIHDL LAGMDLEKIA GDLRSELAST TSELKQKKYL KRLKVVENFM ESGNRPEWMI 250 260 270 280 290 300 MKVVPVIPPD LRPLVPLDGG RFATSDLNDL YRRVINRNNR LKRLIELRAP GIIVRNEKRM 310 320 330 340 350 360 LQEAVDALFD NGRRGRVITG ANKRPLKSLS DMLKGKQGRF RQNLLGKRVD YSGRSVIVTG 370 380 390 400 410 420 PELKLHQCGL PKKMALELFK PFIYARLDAK GYSSTVKQAK KLVEKERPEV WDILDEVIRE 430 440 450 460 470 480 HPVLLNRAPT LHRLGIQAFE PILIEGKAIQ LHPLVCTAFN ADFDGDQMAV HVPLSLEAQL 490 500 510 520 530 540 EARVLMMSTN NILHPASGAP IIVPSQDMVL GLYYLSIVNQ NEPGEGMVFA DMGELQHALE 550 560 570 580 590 600 TKAVTLHAKI KGRFRTVDAE GKVVSKIHDT TPGRMIIGEL LPKNVNVPYE TANQEMTKKN 610 620 630 640 650 660 ISKMIDTVYR HCGQKETVIF CDRIMALGFA HACRAGISFG KDDMLIPDSK IKLVSDTEAL 670 680 690 700 710 720 AKEYEQQYND GLITQGEKYN KVVDAWAKCS EKVADEMMAR IKAVEFEDNG RQKPMNSIYM 730 740 750 760 770 780 MSHSGARGSP TQMRQLAGMR GLMAKPSGEI IETPIISNFK EGLTVLEYFN STHGARKGLA 790 800 810 820 830 840 DTALKTANSG YLTRRLVDVA QDCIVNSVDC GTDKGLTMQP IVDAGQVVAS VGQRVLGRTA 850 860 870 880 890 900 LDDINHPVTG DLLVKAGTLM DERDVEQIEK AGVQSVRIRS ALTCEVRVGV CAVCYGRDLA 910 920 930 940 950 960 RGTPVNQGEA VGVIAAQSIG EPGTQLTMRT FHMGGTAQVV DSSFLEASYE GKVEIRNRNV 970 980 990 1000 1010 1020 VRNSDGQQMV MGRNMAVLIL DEAGKERATH RVTYGSRIFV DDGDKVKRGQ RIAEWDPYTR 1030 1040 1050 1060 1070 1080 PILTEIEGRV AFEDLVDGIS VQETADESTG ITKREVIDWR STPRGNDLKP AIVVQDAKGK 1090 1100 1110 1120 1130 1140 VGKLSKGGDA RFLLSVEAIL SVEPGAQVRP GDVLARIPME SAKTKDITGG LPRVAELFEA 1150 1160 1170 1180 1190 1200 RRPKDHAIIA EIDGTIRFGR DYKNKRRIII EPHDSTLEPV EYLIPKGKPF HLQDGDVIEK 1210 1220 1230 1240 1250 1260 GDYILDGNPA PHDILAIKGV EALASYLVNE IQEVYRLQGV SINDKHIEVI VRQMLQKVEI 1270 1280 1290 1300 1310 1320 TTQGDSTYIP GDHVDVIELE EVNERLIEDG KKPAEGQPVL LGITKASLQT PSFISAASFQ 1330 1340 1350 1360 1370 1380 ETTRVLTEAA VAGKTDMLQG LKENVIVGRL IPAGTGGTMS QIRRIATSRD ELIIDERRKA 1390 SGVEVAEPML ADMTTAAQ

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB47897.1.
RefSeq	NP_102111.1. NC_002678.2.
3D structure databases
ProteinModelPortal	Q98N65.
SMR	Q98N65. Positions 21-166.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1224774.
GenomeReviews	Gene locus mlr0277 in contig BA000012_GR.
KEGG	mlo:mlr0277.
NMPDR	fig|266835.1.peg.216.
PATRIC	22474596. VBIMesLot2464_0220.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG621785.
OMA	DINDKHI.
ProtClustDB	PRK00566.
Family and domain databases
HAMAP	MF_01322. RNApol_bact_RpoC. [Tree]
InterPro	IPR012754. DNA-dir_RpoC_beta_prime. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03046.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. RpoC_TIGR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOC_RHILO

Accession

Primary (citable) accession number: Q98N65

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program