Q98M95 (GATA_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	mll0675

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00120
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP MF_00120		PRO_0000105193
Sites
	Active site	79	1	Charge relay system By similarity
	Active site	187	1	Charge relay system By similarity
	Active site	211	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98M95 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 7D416666B589F0C9	FASTA	521	55,665
	10 20 30 40 50 60 MSDLTRLTIS QARAKLRGKE ITATEITEAY LSAIDRANPA LNAYVAVTGD RARDMAKASD 70 80 90 100 110 120 ARLVKGEGGA LEGIPLGIKD LFGTEGVHTQ ACSHVLDGFK PLYESTVTAN LWADGAVMLG 130 140 150 160 170 180 KLNMDEFAMG SSNETSYYGP VINPWRRSRL DTVVMPTTHQ GDGGFVSAGG TKTQRSLDNA 190 200 210 220 230 240 QLVPGGSSGG SATAVSAFLC AGATATDTGG SIRQPAAFTG TVGIKPTYGR CSRWGIVAFA 250 260 270 280 290 300 SSLDQAGPIA RDVRDAAILL KSMASVDPKD TTSVDRPVPD YEAAIGKPIK GMKVGIPKEY 310 320 330 340 350 360 RVDGMPEEIE ALWQKGIAWL RDAGAEIVDI SLPHTKYALP AYYIVAPAEA SSNLARYDGV 370 380 390 400 410 420 RYGLRVPGKD IVEMYEKTRA AGFGREVKRR IMIGTYVLSA GYYDAYYLQA QKVRNLIKRD 430 440 450 460 470 480 FENVFAAGVD VILTPATPSA AFGIADEDMA ADPVKMYLND IFTVTVNMAG LPGIAVPAGL 490 500 510 520 DPKGLPLGLQ LIGRPFEEET LFQTAAVIEQ AAGTFQPEKW W

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB48218.1.
RefSeq	NP_102432.1. NC_002678.2.
3D structure databases
ProteinModelPortal	Q98M95.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1225095.
GenomeReviews	Gene locus mll0675 in contig BA000012_GR.
KEGG	mlo:mll0675.
NMPDR	fig|266835.1.peg.537.
PATRIC	22475244. VBIMesLot2464_0542.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG481888.
OMA	FDEETLF.
ProtClustDB	PRK00012.
Family and domain databases
HAMAP	MF_00120. GatA. [Tree]
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
Gene3D	G3DSA:3.90.1300.10. Amidase_sig_enz. 2 hits.
KO	K02433.
PANTHER	PTHR11895. Amidase. 1 hit.
Pfam	PF01425. Amidase. 2 hits. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_RHILO

Accession

Primary (citable) accession number: Q98M95

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program