Q98M73 (GLPK_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	mll0700

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Glycerol kinase HAMAP MF_00186		PRO_0000059482
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98M73 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 2A360DECD38EE8A6	FASTA	496	53,804
	10 20 30 40 50 60 MSGFVLAIDQ GTTSTRAILF DGQMKVAGSG QKEFAQHYPA SGWVEHDPEE IWASVVTTVK 70 80 90 100 110 120 AALKNAGKAA SDVAAIGITN QRETVVVWDR ATGKPIHNAI VWQDRRTAPL CQKLKKQGLE 130 140 150 160 170 180 KKFTRKTGLL LDPYFSGTKI AWILDKVKGG RKRAEKGELL AGTIDSFLIW RLTGGKVHAT 190 200 210 220 230 240 DATNASRTLV YNIAENAWDD ELLAILNIPA KLLPQVKDCA DDYGVTERDL FGAEIRILGV 250 260 270 280 290 300 AGDQHAATIG QACFEPGMMK STYGTGCFAL LNTGSDLVRS KNRLLTTIAY RLNGKTTYAL 310 320 330 340 350 360 EGSIFIAGAA VQWLRDGIKV IGKAEQSGKL ADEADPTQNV YLVPAFVGLG APHWDAEARG 370 380 390 400 410 420 AIFGLTRNSG PAEFARAALE AVAYQTRDLL DAMRKDWKGT SAKTVLRVDG GMVASDWTMQ 430 440 450 460 470 480 RLADILDAPV DRPTILETTA LGAAWLAGSK AGVWPKAKEF AKSWALERRF KPEMDIATRS 490 AKLAGWRDAV RRTLSA

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB48240.1.
RefSeq	NP_102454.1. NC_002678.2.
3D structure databases
ProteinModelPortal	Q98M73.
SMR	Q98M73. Positions 1-494.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1225117.
GenomeReviews	Gene locus mll0700 in contig BA000012_GR.
KEGG	mlo:mll0700.
NMPDR	fig|266835.1.peg.559.
PATRIC	22475290. VBIMesLot2464_0564.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	CYQTRDL.
ProtClustDB	CLSK863056.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_RHILO

Accession

Primary (citable) accession number: Q98M73

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program