Reviewed, UniProtKB/Swiss-Prot Q98LB3 (ILVD2_RHILO)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 2
      Short name=DAD 2
    EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	mll1102

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000103497
Sites
	Metal binding	141	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	213	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q98LB3-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 2E2191A3224F86F8	FASTA	586	61,957
	10 20 30 40 50 60 MRQAAPPAAR MRMDAKVISK AKLPSRHVTV GPARAPHRSY LYAMGLSAAE IAQPLVGVAS 70 80 90 100 110 120 CWNEAAPCNI SLMRQAQVVK KGVAAANGTP REFCTITVTD GIAMGHQGMK SSLVSREVIA 130 140 150 160 170 180 DSVELTMRGH CYDALVGLAG CDKSLPGMMM AMVRLNVPSI FIYGGSILPG SYRGRQITVQ 190 200 210 220 230 240 DVFEAVGQHS VGTIGDAELL EIEQAACPSA GSCGAQFTAN TMATVAEAIG LALPYSCGAP 250 260 270 280 290 300 APYEMRDRFN FASGEKIMEL IAKNIRPRDI ITLKALENAA TVVSATGGST NAALHLPAIA 310 320 330 340 350 360 HEAGIKFDLF DVARIFEKTP YIADLKPGGK YVAKDMFEAG GIPLLMKTLL DHGYLHGDCL 370 380 390 400 410 420 TVTGRTLAEN MEHVAWNEHQ DVVRPANRPI TQTGGVVGLK GNLAPEGAIV KVAGMTELKF 430 440 450 460 470 480 SGPARCFDSE EECFEAVTQR NYREGEVLVI RYEGPRGGPG MREMLSTTAA LYGQGMGGKV 490 500 510 520 530 540 ALITDGRFSG ATRGFCIGHV GPEAAVGGPI GLIRDGDVIS IDAVNGTIEV ALSDSELAAR 550 560 570 580 AKTWKARTTD YQSGAIWKYA QTVGSARDGA VTHPGGAKET HCYADI

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB48550.1.
RefSeq	NP_102764.1.
3D structure databases
SMR	Q98LB3. Positions 85-574.
ModBase	Search...
Genome annotation databases
GeneID	1225427.
GenomeReviews	Gene locus mll1102 in contig BA000012_GR.
KEGG	mlo:mll1102.
NMPDR	fig|266835.1.peg.869.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	NEAAPCN.
Enzyme and pathway databases
BRENDA	4.2.1.9. 3315.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_RHILO

Accession

Primary (citable) accession number: Q98LB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)