Q98KT9 (MTKA_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable malate--CoA ligase subunit beta
EC=6.2.1.9

Alternative name(s):
MTK-beta
Malate thiokinase
Malyl-CoA synthetase

Gene names

Name:	mtkA
Ordered Locus Names:	mlr1324

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + malate + CoA = ADP + phosphate + malyl-CoA. HAMAP MF_00558
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	One-carbon metabolism; formaldehyde assimilation via serine pathway. HAMAP MF_00558
Subunit structure	Heterotetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the succinate/malate CoA ligase beta subunit family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW malate-CoA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Probable malate--CoA ligase subunit beta HAMAP MF_00558		PRO_0000102889
Regions
	Domain	9 – 244	236	ATP-grasp
	Nucleotide binding	35 – 108	74	ATP By similarity
Sites
	Metal binding	197	1	Magnesium or manganese By similarity
	Metal binding	199	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98KT9 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: A1D573E98E5CB24C	FASTA	394	42,482
	10 20 30 40 50 60 MDIHEYQAKE LLARHGVHVP RGGLAYSPEQ ATYRAREIGG GKWVLKAQVH SGARGKAGGI 70 80 90 100 110 120 KLCANDEEIS SAAEAMLGRK LVTQQTGPRG KLISRLYLEE AVDIAQELYV GFVLDRKEER 130 140 150 160 170 180 VMIVASAAGG MEIEDIVEKE PNSILRTSVD PGVGMQRFQA REIAFGLGLD HNLIGKATET 190 200 210 220 230 240 IFSCYQVFRD YDASMLEINP LVVTRDGNLI ALDAKMSFDE NALFRRPEIS ELRDKSQEDP 250 260 270 280 290 300 RETFASDRGL SYVGLDGNIG CIINGAGLAM ATMDMIKIAG GEPANFLDIG GGASPERVAK 310 320 330 340 350 360 SFRAVLGDKN VETILVNIFA GINRCDWVAE GVIKAIREVG VNVPLVVRLS GTKAEEGRRI 370 380 390 LADSGEAVIV ADTLAEAAEK AVAAWRAAAK KKAA

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB48725.1.
RefSeq	NP_102939.1. NC_002678.2.
3D structure databases
ProteinModelPortal	Q98KT9.
SMR	Q98KT9. Positions 1-384.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1225602.
GenomeReviews	Gene locus mlr1324 in contig BA000012_GR.
KEGG	mlo:mlr1324.
NMPDR	fig|266835.1.peg.1044.
PATRIC	22476316. VBIMesLot2464_1070.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG315432.
OMA	MSFDENA.
ProtClustDB	PRK14046.
Family and domain databases
HAMAP	MF_00558. Succ_CoA_beta. Divergent sequence. [Tree]
InterPro	IPR013650. ATP-grasp_succ-CoA_synth-type. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR005811. CoA_ligase. IPR017866. Succ-CoA_synthase_bsu_CS. IPR005809. Succ_CoA_synthase_bsu. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.261. Succinyl-CoA_synth-like. 1 hit.
KO	K14067.
PANTHER	PTHR11815. CoA_lig_beta. 1 hit.
Pfam	PF08442. ATP-grasp_2. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
PIRSF	PIRSF001554. SucCS_beta. 1 hit.
SUPFAM	SSF52210. CoA_ligase. 1 hit.
TIGRFAMs	TIGR01016. SucCoAbeta. 1 hit.
PROSITE	PS50975. ATP_GRASP. False negative. PS01217. SUCCINYL_COA_LIG_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTKA_RHILO

Accession

Primary (citable) accession number: Q98KT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program