Q98KR3 (NUOH_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit H
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H

Gene names

Name:	nuoH
Ordered Locus Names:	mll1361

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone By similarity. HAMAP MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01350
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP MF_01350.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	NADH-quinone oxidoreductase subunit H HAMAP MF_01350		PRO_0000244937
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Transmembrane	82 – 102	21	Helical; Potential
	Transmembrane	115 – 135	21	Helical; Potential
	Transmembrane	161 – 181	21	Helical; Potential
	Transmembrane	198 – 218	21	Helical; Potential
	Transmembrane	248 – 268	21	Helical; Potential
	Transmembrane	283 – 303	21	Helical; Potential
	Transmembrane	321 – 341	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q98KR3 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 1092F351BD97EC57	FASTA	347	38,371
	10 20 30 40 50 60 MDTFFSFYVL PALLILLKSV VLIVVLLIFV AYILYADRKI WAAVQLRRGP NVVGPWGTLQ 70 80 90 100 110 120 AFADLLKFVF KEPVIPSGAN KGVFLLAPLV SAVLAISAWA VIPVNQGWAI ANVNVGILYV 130 140 150 160 170 180 FAISSLEVYG VIMGGWASNS KYPFLGALRS AAQMVSYEVS IGFVIVTVLL TAGSLNLSDI 190 200 210 220 230 240 VLSQHDGIGT RLGLPNTFLD WNWLALFPMF IIFFISALAE TNRPPFDLVE AESELVAGHM 250 260 270 280 290 300 VEYSSTPFLL FFLGEYVAIV LMCALATILF LGGWLPPFDF VPFTWVPGVI WFVLKVCFVF 310 320 330 340 FGISMVKAFV PRYRYDQLMR LGWKVFLPIS LFMVVATAAF LKITGFA

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB48751.1.
RefSeq	NP_102965.1. NC_002678.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1225628.
GenomeReviews	Gene locus mll1361 in contig BA000012_GR.
KEGG	mlo:mll1361.
NMPDR	fig|266835.1.peg.1070.
PATRIC	22476370. VBIMesLot2464_1096.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG727670.
OMA	AGFMVEY.
ProtClustDB	PRK06076.
Family and domain databases
HAMAP	MF_01350. NDH1_NuoH. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
KO	K00337.
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. False negative. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOH_RHILO

Accession

Primary (citable) accession number: Q98KR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program