Q98KB4 (MURC_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	mll1553

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182139
Regions
	Nucleotide binding	114 – 120	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q98KB4 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 1E54A5E710BD82E4	FASTA	466	50,167
	10 20 30 40 50 60 MKMPQTIGLV HFIGIGGIGM SGIAEVLHNL GYKVQGSDQA DSANVQRLRD KGIECFVGHK 70 80 90 100 110 120 AENLGDAEVV VVSTAIKKTN PELKAAREKL LPIVRRAEML AELMRFRQAV AIGGTHGKTT 130 140 150 160 170 180 TTSMVATLLE AGGLDPTVIN GGIINAYGTN ARMGDGEWMV VEADESDGTF LKLPADIAVV 190 200 210 220 230 240 TNIDPEHLDH YGSFDKVREA FRQFVENVPF YGFGVMCTDH PEVQALVGRI EDRRVITYGE 250 260 270 280 290 300 NAQADVRFTN HRMAGATSEF DVVIRDRKTG SQSTISGLRL PMPGRHNVSN ATAAIAVAHE 310 320 330 340 350 360 LGLSGEAIKK GLSSFAGVKR RFTHTGSWNG VDVFDDYGHH PVEISAVLKA ARSATKGRVI 370 380 390 400 410 420 AIAQPHRFTR LHDLFNEFSA CFNDADTVIV APVYAAGEDP IDGVSSDELV SRIRAGGHRD 430 440 450 460 ARYIEGPTAI APIIRGLAKP GDFVVFLGAG NITQWAYALP KELAAS

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB48900.1.
RefSeq	NP_103114.1. NC_002678.2.
3D structure databases
ProteinModelPortal	Q98KB4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1225777.
GenomeReviews	Gene locus mll1553 in contig BA000012_GR.
KEGG	mlo:mll1553.
NMPDR	fig|266835.1.peg.1219.
PATRIC	22476682. VBIMesLot2464_1251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG476594.
OMA	YQPHRYS.
ProtClustDB	PRK00421.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_RHILO

Accession

Primary (citable) accession number: Q98KB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program