ID   DDLA_RHILO              Reviewed;         357 AA.
AC   Q98JS0;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   16-JUN-2009, entry version 43.
DE   RecName: Full=D-alanine--D-alanine ligase A;
DE            EC=6.3.2.4;
DE   AltName: Full=D-alanylalanine synthetase A;
DE   AltName: Full=D-Ala-D-Ala ligase A;
GN   Name=ddlA; OrderedLocusNames=mll1812;
OS   Rhizobium loti (Mesorhizobium loti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Phyllobacteriaceae; Mesorhizobium.
OX   NCBI_TaxID=381;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MAFF303099;
RX   MEDLINE=21082930; PubMed=11214968; DOI=10.1093/dnares/7.6.331;
RA   Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T.,
RA   Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Mochizuki Y., Nakayama S., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takeuchi C., Yamada M., Tabata S.;
RT   "Complete genome structure of the nitrogen-fixing symbiotic bacterium
RT   Mesorhizobium loti.";
RL   DNA Res. 7:331-338(2000).
CC   -!- FUNCTION: Cell wall formation (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-
CC       alanyl-D-alanine.
CC   -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC       similarity).
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; BA000012; BAB49095.1; -; Genomic_DNA.
DR   RefSeq; NP_103309.1; -.
DR   HSSP; P25051; 1E4E.
DR   GeneID; 1225970; -.
DR   GenomeReviews; BA000012_GR; mll1812.
DR   KEGG; mlo:mll1812; -.
DR   NMPDR; fig|266835.1.peg.1413; -.
DR   HOGENOM; Q98JS0; -.
DR   OMA; Q98JS0; GREIECG.
DR   BRENDA; 6.3.2.4; 3315.
DR   GO; GO:0005618; C:cell wall; IEA:InterPro.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007047; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   HAMAP; MF_00047; -; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR000291; D-Ala_lig_Van_CS.
DR   InterPro; IPR005905; D_ala_D_ala.
DR   InterPro; IPR011095; Dala_Dala_lig_C.
DR   InterPro; IPR011127; Dala_Dala_lig_N.
DR   InterPro; IPR013817; Pre-ATP_grasp.
DR   Gene3D; G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
DR   Gene3D; G3DSA:3.40.50.20; Pre-ATP_grasp; 1.
DR   Pfam; PF07478; Dala_Dala_lig_C; 1.
DR   Pfam; PF01820; Dala_Dala_lig_N; 1.
DR   TIGRFAMs; TIGR01205; D_ala_D_alaTIGR; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00843; DALA_DALA_LIGASE_1; 1.
DR   PROSITE; PS00844; DALA_DALA_LIGASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell shape; Cell wall biogenesis/degradation;
KW   Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.
FT   CHAIN         1    357       D-alanine--D-alanine ligase A.
FT                                /FTId=PRO_0000177862.
FT   DOMAIN      143    348       ATP-grasp.
FT   NP_BIND     171    226       ATP (By similarity).
FT   METAL       302    302       Magnesium or manganese 1 (By similarity).
FT   METAL       315    315       Magnesium or manganese 1 (By similarity).
FT   METAL       315    315       Magnesium or manganese 2 (By similarity).
FT   METAL       317    317       Magnesium or manganese 2 (By similarity).
SQ   SEQUENCE   357 AA;  37875 MW;  1C096AE7FCD57C8B CRC64;
     MKEKTRVAIL YGGRSAEHDV SRLSAANVLK AIDRTRYEVV PIAISRDGRW RLQPSSEAGA
     DGAGAQVPED GVEVALLPGG KGRLVALTKN GEQPDPVDVV FPVLHGPFGE DGSVQGYAEV
     ADVAYVGCGI LASAAAMDKD VAKRLLREAG LAVARSVTVL KGDVGSFQEI AGALGLPFFA
     KPARQGSSFG VSKVHDRDGF EQAVETALRY DSKALIEEFV DGREIECSVL ERADGSLTVS
     PPGEIIPADK HGFYTYEAKY FDAEGAVVKV PADVPADVVA RTSEMAARAF RALGCEAMAR
     VDFFLRADGS LLVNEVNTLP GFTDISMYAK ALAAAGIGYS KVIDVLIEHA LARHRAR
//