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Q98JS0 (DDLA_RHILO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase A

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase A
D-alanylalanine synthetase A
Gene names
Name:ddlA
Ordered Locus Names:mll1812
OrganismRhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]
Taxonomic identifier266835 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357D-alanine--D-alanine ligase A HAMAP-Rule MF_00047
PRO_0000177862

Regions

Domain143 – 348206ATP-grasp
Nucleotide binding171 – 22656ATP By similarity

Sites

Metal binding3021Magnesium or manganese 1 By similarity
Metal binding3151Magnesium or manganese 1 By similarity
Metal binding3151Magnesium or manganese 2 By similarity
Metal binding3171Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q98JS0 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 1C096AE7FCD57C8B

FASTA35737,875
        10         20         30         40         50         60 
MKEKTRVAIL YGGRSAEHDV SRLSAANVLK AIDRTRYEVV PIAISRDGRW RLQPSSEAGA 

        70         80         90        100        110        120 
DGAGAQVPED GVEVALLPGG KGRLVALTKN GEQPDPVDVV FPVLHGPFGE DGSVQGYAEV 

       130        140        150        160        170        180 
ADVAYVGCGI LASAAAMDKD VAKRLLREAG LAVARSVTVL KGDVGSFQEI AGALGLPFFA 

       190        200        210        220        230        240 
KPARQGSSFG VSKVHDRDGF EQAVETALRY DSKALIEEFV DGREIECSVL ERADGSLTVS 

       250        260        270        280        290        300 
PPGEIIPADK HGFYTYEAKY FDAEGAVVKV PADVPADVVA RTSEMAARAF RALGCEAMAR 

       310        320        330        340        350 
VDFFLRADGS LLVNEVNTLP GFTDISMYAK ALAAAGIGYS KVIDVLIEHA LARHRAR 

« Hide

References

[1]"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti."
Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. expand/collapse author list , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S.
DNA Res. 7:331-338(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MAFF303099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000012 Genomic DNA. Translation: BAB49095.1.
RefSeqNP_103309.1. NC_002678.2.

3D structure databases

ProteinModelPortalQ98JS0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266835.mll1812.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB49095; BAB49095; BAB49095.
GeneID1225970.
KEGGmlo:mll1812.
PATRIC22477098. VBIMesLot2464_1458.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAPHELPRI.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycMLOT266835:GJ9L-1432-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDLA_RHILO
AccessionPrimary (citable) accession number: Q98JS0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways