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Protein

Ribose-phosphate pyrophosphokinase

Gene

prs

Organism
Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).UniRule annotation

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 Mg2+ ions per subunit.UniRule annotation

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase (prs)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi127Magnesium 1UniRule annotation1
Metal bindingi168Magnesium 2UniRule annotation1
Active sitei191UniRule annotation1
Binding sitei193Ribose-5-phosphateUniRule annotation1
Binding sitei217Ribose-5-phosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 36ATPUniRule annotation3
Nucleotide bindingi93 – 94ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00087; UER00172

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinaseUniRule annotation (EC:2.7.6.1UniRule annotation)
Short name:
RPPKUniRule annotation
Alternative name(s):
5-phospho-D-ribosyl alpha-1-diphosphateUniRule annotation
Phosphoribosyl diphosphate synthaseUniRule annotation
Phosphoribosyl pyrophosphate synthaseUniRule annotation
Short name:
P-Rib-PP synthaseUniRule annotation
Short name:
PRPP synthaseUniRule annotation
Short name:
PRPPaseUniRule annotation
Gene namesi
Name:prsUniRule annotation
Ordered Locus Names:mlr2685
OrganismiMesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001411801 – 311Ribose-phosphate pyrophosphokinaseAdd BLAST311

Proteomic databases

PRIDEiQ98HW3

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi266835.mlr2685

Structurei

3D structure databases

ProteinModelPortaliQ98HW3
SMRiQ98HW3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 225Ribose-5-phosphate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C5T Bacteria
COG0462 LUCA
HOGENOMiHOG000210449
KOiK00948
OMAiFGWARQD
OrthoDBiPOG091H018X

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
HAMAPiMF_00583_B RibP_PPkinase_B, 1 hit
InterProiView protein in InterPro
IPR000842 PRib_PP_synth_CS
IPR029099 Pribosyltran_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005946 Rib-P_diPkinase
IPR037515 Rib-P_diPkinase_bac
PfamiView protein in Pfam
PF14572 Pribosyl_synth, 1 hit
PF13793 Pribosyltran_N, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01251 ribP_PPkin, 1 hit
PROSITEiView protein in PROSITE
PS00114 PRPP_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q98HW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLFAGNSNR VLAEAVARYL NIPLGKATVR RFADQEIFVE IQENVRGEDV
60 70 80 90 100
FILQSTSFPT NDHLMELLIM IDAFMRSSAK RITAVIPYFG YARQDRRASG
110 120 130 140 150
RTPISAKLVA NMITRAGVDR VLTLDLHAGQ IQGFFDIPTD NLFSVPVMAR
160 170 180 190 200
DVKAKYKQLG NVVVVSPDIG GVVRARALAK RFDAQLAIVD KRRERPGESE
210 220 230 240 250
VMNIIGAVAG KDCLLIDDIV DSGGTLCNAA DALLANGATS VTAYITHGVL
260 270 280 290 300
SGGAVARISG SKLQELVITD SIQPTQGVLD APNIRVISIA DLMGEAISRT
310
ATEESVSSLF D
Length:311
Mass (Da):33,519
Last modified:October 1, 2001 - v1
Checksum:i7DE35FB790E512F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA Translation: BAB49753.1
RefSeqiWP_010911102.1, NC_002678.2

Genome annotation databases

EnsemblBacteriaiBAB49753; BAB49753; BAB49753
KEGGimlo:mlr2685

Similar proteinsi

Entry informationi

Entry nameiKPRS_RHILO
AccessioniPrimary (citable) accession number: Q98HW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: October 1, 2001
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health