Q98GG1 (FOSX_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fosfomycin resistance protein FosX

Gene names

Name:	fosX
Ordered Locus Names:	mlr3345

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium › Rhizobium loti

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydration of fosfomycin. Ref.2
Cofactor	Manganese. Ref.2
Subunit structure	Homodimer. Ref.2
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the fosfomycin resistance protein family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 139

139

Fosfomycin resistance protein FosX

PRO_0000164047

Sites

Active site

44

1

Proton acceptor Probable

Metal binding

7

1

Manganese

Metal binding

69

1

Manganese

Metal binding

118

1

Manganese

Experimental info

Mutagenesis

44

1

E → G: Decrease in activity. Ref.2

Secondary structure

1

.

.

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.

.

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.

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.

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.

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.

.

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139

Helix Strand Turn

Details...

Beta strand

2 – 13

12

Helix

15 – 26

12

Beta strand

29 – 33

5

Helix

34 – 36

3

Beta strand

44 – 49

6

Beta strand

52 – 58

7

Beta strand

69 – 73

5

Helix

76 – 78

3

Helix

79 – 89

11

Beta strand

106 – 110

5

Beta strand

116 – 120

5

Helix

124 – 129

6

Sequences

	Sequence		Length	Mass (Da)
	Q98GG1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 8F9138A570B900F2	FASTA	139	16,181
	10 20 30 40 50 60 MIEGLSHMTF IVRDLERMTR ILEGVFDARE VYASDTEQFS LSREKFFLIG DIWVAIMQGE 70 80 90 100 110 120 KLAERSYNHI AFKIDDADFD RYAERVGKLG LDMRPPRPRV EGEGRSIYFY DDDNHMFELH 130 TGTLTERLAR KAKGLEAAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.
[2]	"Mechanistic diversity of fosfomycin resistance in pathogenic microorganisms." Fillgrove K.L., Pakhomova S., Newcomer M.E., Armstrong R.N. J. Am. Chem. Soc. 125:15730-15731(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS), FUNCTION, COFACTOR, SUBUNIT, MUTAGENESIS OF GLU-44. Strain: MAFF303099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000012 Genomic DNA. Translation: BAB50255.1.

RefSeq

NP_104469.1. NC_002678.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1R9C	X-ray	1.83	A/B	1-139	[»]

ProteinModelPortal

Q98GG1.

SMR

Q98GG1. Positions 1-130.

ModBase

Search...

Protein-protein interaction databases

STRING

266835.mlr3345.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAB50255; BAB50255; BAB50255.

GeneID

1227130.

KEGG

mlo:mlr3345.

PATRIC

22479521. VBIMesLot2464_2664.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0346.

HOGENOM

HOG000232023.

KO

K00799.

OMA

MEDANIL.

ProtClustDB

CLSK863737.

Enzyme and pathway databases

BioCyc

MLOT266835:GJ9L-2596-MONOMER.

Family and domain databases

InterPro

IPR004360. Glyas_Fos-R_dOase_dom.
[Graphical view]

Pfam

PF00903. Glyoxalase. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q98GG1.

Entry information

Entry name

FOSX_RHILO

Accession

Primary (citable) accession number: Q98GG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program