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Q98F72 (RUVC_RHILO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:mll3901
OrganismRhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]
Taxonomic identifier266835 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium

Protein attributes

Sequence length169 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 169169Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000183125

Sites

Metal binding111Magnesium By similarity
Metal binding711Magnesium By similarity
Metal binding1431Magnesium By similarity
Metal binding1461Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q98F72 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 4C8D36002C8F1518

FASTA16917,840
        10         20         30         40         50         60 
MGDAIRIIGI DPGLRRTGWG IVESLGNSLR FVASGTVRSE DKAALATRLC QLHDGLAEVL 

        70         80         90        100        110        120 
HAAMPHEAAV EQTFVNKDAV ATLKLGQARG IAMLVPARAG LVVAEYAPNA VKKAVIGVGH 

       130        140        150        160 
GDKKQIHMMV KVLLPKATFD TEHAADALAI AICHAHHRQS AAYRMALAG 

« Hide

References

[1]"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti."
Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. expand/collapse author list , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S.
DNA Res. 7:331-338(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MAFF303099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000012 Genomic DNA. Translation: BAB50695.1.
RefSeqNP_104909.1. NC_002678.2.

3D structure databases

ProteinModelPortalQ98F72.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266835.mll3901.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB50695; BAB50695; BAB50695.
GeneID1227570.
KEGGmlo:mll3901.
PATRIC22480433. VBIMesLot2464_3105.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAARGACIT.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycMLOT266835:GJ9L-3051-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUVC_RHILO
AccessionPrimary (citable) accession number: Q98F72
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families