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Protein

Glutamate 5-kinase 1

Gene

proB1

Organism
Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase 1 (proB1), Glutamate 5-kinase 2 (proB2)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATPUniRule annotation1
Binding sitei53SubstrateUniRule annotation1
Binding sitei140SubstrateUniRule annotation1
Binding sitei152Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 173ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinase 1UniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinase 1UniRule annotation
Short name:
GK 1UniRule annotation
Gene namesi
Name:proB1UniRule annotation
Ordered Locus Names:mll4011
OrganismiMesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097131 – 378Glutamate 5-kinase 1Add BLAST378

Proteomic databases

PRIDEiQ98EZ4

Interactioni

Protein-protein interaction databases

STRINGi266835.mll4011

Structurei

3D structure databases

ProteinModelPortaliQ98EZ4
SMRiQ98EZ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini278 – 355PUAUniRule annotationAdd BLAST78

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiNMINCYE
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q98EZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSLKKYRRI TVKIGSALLV DRASGLKRDW LDSLADDIAV LAQGGAEILV
60 70 80 90 100
VSSGAIALGR TILGLGKRAL KLEESQAAAA VGQIALAGAW SDALGRGALK
110 120 130 140 150
SGQILLTLGD TEERRRYLNA RATISTLLKM KAVPVINEND TVATSEIRYG
160 170 180 190 200
DNDRLAARVA TMMGADLLVL LSDIDGLYTA PPARDPKAKF IPVVDRITPD
210 220 230 240 250
IEAMAGAAAS ELSRGGMRTK LDAGKIATAA GTAMIITSGT RLSPLMAIER
260 270 280 290 300
GERATFFRPS ANPVKGYKTW IAGQLEPAGR LTVDAGAVGA LASGKSLLPA
310 320 330 340 350
GVKLVSGNFS RGDTVAILSP DGREIARGLV AYDAADAVRI AGLKTAEIET
360 370
VLGYEARSAM IHRDDLVVSH AGGDISGG
Length:378
Mass (Da):39,353
Last modified:September 26, 2001 - v1
Checksum:i44B68AECBF9A8B2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA Translation: BAB50773.1
RefSeqiWP_010912116.1, NC_002678.2

Genome annotation databases

EnsemblBacteriaiBAB50773; BAB50773; BAB50773
KEGGimlo:mll4011

Similar proteinsi

Entry informationi

Entry nameiPROB1_RHILO
AccessioniPrimary (citable) accession number: Q98EZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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