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Q98EU7 (SYL_RHILO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:mll4077
OrganismRhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]
Taxonomic identifier266835 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium

Protein attributes

Sequence length875 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 875875Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152070

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif631 – 6355"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q98EU7 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 3A2F64BC5199FF20

FASTA87598,134
        10         20         30         40         50         60 
MATERYNPRA SEPKWQKAWA EKKLFEARND DPKPKYYVLE MFPYPSGRIH IGHTRNYTMG 

        70         80         90        100        110        120 
DVVARYKRAK GFNVLHPMGW DAFGMPAENA AMQNKVHPKE WTYQNIATMR EQLKVMGLSL 

       130        140        150        160        170        180 
DWAREFATCD VDYYHRQQML FLDFVEKGLV TRKSSKVNWD PEDMTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GALVEQRELT QWFFKITDFA QDLLDSLEGL DEWPEKVKLM QQNWIGRSEG LLIRWPLASD 

       250        260        270        280        290        300 
VAGEHELEVY TTRPDTIFGA SFMAVAADHP LAKKAAETNP ALAKFIDEVR HMGTSVAALE 

       310        320        330        340        350        360 
TAEKKGFDTG IRVVHPFDDS WTLPVYVANF VLMEYGTGAI FGCPSGDQRD LDFANKYGLP 

       370        380        390        400        410        420 
VIPVVMPEGG DAKSFQITEE AYVDDGVMIN SRFLDGMKPD RAFDEVAKLL EQKTIGNRPM 

       430        440        450        460        470        480 
AERKVNFRLR DWGISRQRYW GCPIPMIHCE DCGVVPVPKA DLPVKLPDDV DFDRPGNPLD 

       490        500        510        520        530        540 
RHPTWRHVKC PQCGRDARRE TDTMDTFVDS SWYFARFTAP WAYEPTDPRA ANEWLPVDQY 

       550        560        570        580        590        600 
IGGIEHAILH LLYSRFFTRA MRATGHVDLA EPFKGLFTQG MVVHETYRVG GASNNGRWLS 

       610        620        630        640        650        660 
PAEVRIEDAE GKRRAIEIAT GEEAAIGSLE KMSKSKKNTV SPEDITDGYG ADTARWLMLS 

       670        680        690        700        710        720 
DSPPEGDVEW TDDGAAGAHR FMQRIWRLVS TAAETLAGVK PAAADSGEAG AVRKATHKIL 

       730        740        750        760        770        780 
KAVGEDIEKL GFNRAIARIY ELANVLTTPL NQVAEGKADP ALQGACREAV EILVHLIAPV 

       790        800        810        820        830        840 
MPHLAEECWE TLGGTDLVAE RPWPAFDPAL VVDNEVTYPV QVNGKKRGDL TIARDADQGA 

       850        860        870 
VEKAVLALDF VQKALEGKAP RKVIIVPQRI VNVVA 

« Hide

References

[1]"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti."
Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. expand/collapse author list , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S.
DNA Res. 7:331-338(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MAFF303099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000012 Genomic DNA. Translation: BAB50820.1.
RefSeqNP_105034.1. NC_002678.2.

3D structure databases

ProteinModelPortalQ98EU7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266835.mll4077.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB50820; BAB50820; BAB50820.
GeneID1227695.
KEGGmlo:mll4077.
PATRIC22480675. VBIMesLot2464_3225.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHELEVYT.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycMLOT266835:GJ9L-3177-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RHILO
AccessionPrimary (citable) accession number: Q98EU7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries