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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei634 – 6341ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLOT266835:GJ9L-3177-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:mll4077
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
ProteomesiUP000000552 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875Leucine--tRNA ligasePRO_0000152070Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266835.mll4077.

Structurei

3D structure databases

ProteinModelPortaliQ98EU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi631 – 6355"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q98EU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATERYNPRA SEPKWQKAWA EKKLFEARND DPKPKYYVLE MFPYPSGRIH
60 70 80 90 100
IGHTRNYTMG DVVARYKRAK GFNVLHPMGW DAFGMPAENA AMQNKVHPKE
110 120 130 140 150
WTYQNIATMR EQLKVMGLSL DWAREFATCD VDYYHRQQML FLDFVEKGLV
160 170 180 190 200
TRKSSKVNWD PEDMTVLANE QVIDGRGWRS GALVEQRELT QWFFKITDFA
210 220 230 240 250
QDLLDSLEGL DEWPEKVKLM QQNWIGRSEG LLIRWPLASD VAGEHELEVY
260 270 280 290 300
TTRPDTIFGA SFMAVAADHP LAKKAAETNP ALAKFIDEVR HMGTSVAALE
310 320 330 340 350
TAEKKGFDTG IRVVHPFDDS WTLPVYVANF VLMEYGTGAI FGCPSGDQRD
360 370 380 390 400
LDFANKYGLP VIPVVMPEGG DAKSFQITEE AYVDDGVMIN SRFLDGMKPD
410 420 430 440 450
RAFDEVAKLL EQKTIGNRPM AERKVNFRLR DWGISRQRYW GCPIPMIHCE
460 470 480 490 500
DCGVVPVPKA DLPVKLPDDV DFDRPGNPLD RHPTWRHVKC PQCGRDARRE
510 520 530 540 550
TDTMDTFVDS SWYFARFTAP WAYEPTDPRA ANEWLPVDQY IGGIEHAILH
560 570 580 590 600
LLYSRFFTRA MRATGHVDLA EPFKGLFTQG MVVHETYRVG GASNNGRWLS
610 620 630 640 650
PAEVRIEDAE GKRRAIEIAT GEEAAIGSLE KMSKSKKNTV SPEDITDGYG
660 670 680 690 700
ADTARWLMLS DSPPEGDVEW TDDGAAGAHR FMQRIWRLVS TAAETLAGVK
710 720 730 740 750
PAAADSGEAG AVRKATHKIL KAVGEDIEKL GFNRAIARIY ELANVLTTPL
760 770 780 790 800
NQVAEGKADP ALQGACREAV EILVHLIAPV MPHLAEECWE TLGGTDLVAE
810 820 830 840 850
RPWPAFDPAL VVDNEVTYPV QVNGKKRGDL TIARDADQGA VEKAVLALDF
860 870
VQKALEGKAP RKVIIVPQRI VNVVA
Length:875
Mass (Da):98,134
Last modified:September 30, 2001 - v1
Checksum:i3A2F64BC5199FF20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB50820.1.
RefSeqiNP_105034.1. NC_002678.2.

Genome annotation databases

EnsemblBacteriaiBAB50820; BAB50820; BAB50820.
GeneIDi1227695.
KEGGimlo:mll4077.
PATRICi22480675. VBIMesLot2464_3225.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB50820.1.
RefSeqiNP_105034.1. NC_002678.2.

3D structure databases

ProteinModelPortaliQ98EU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266835.mll4077.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB50820; BAB50820; BAB50820.
GeneIDi1227695.
KEGGimlo:mll4077.
PATRICi22480675. VBIMesLot2464_3225.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciMLOT266835:GJ9L-3177-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MAFF303099.

Entry informationi

Entry nameiSYL_RHILO
AccessioniPrimary (citable) accession number: Q98EU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 19, 2003
Last sequence update: September 30, 2001
Last modified: March 31, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.