Reviewed, UniProtKB/Swiss-Prot Q98E34 (DNAE2_RHILO)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	mlr4428

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	1118 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1118	1118	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103394

Sequences

	Sequence		Length	Mass (Da)
	Q98E34-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 3D66F8E487FDF6EA	FASTA	1,118	125,208
	10 20 30 40 50 60 MNALTVIPYA EFGIQSNFSF LRGASKPEEL VVAAKLMGFS AIGLADRNTV AGVVRAWQQA 70 80 90 100 110 120 KVEKLAYHPG CRLVFGDGTP DILAYPRNRA GWGHLCRMLT QANLRDETEK GATLLQRDDL 130 140 150 160 170 180 LEWGDLMSLA VLPDLAAGAE DSLALLGQIK DRFGRALRLG VSPCYAGYDR FRIEQAAALA 190 200 210 220 230 240 QVAGIPLMAT NDVLYHTAER RPLQDVLTAI RLNTPVAEVG LELSANAERH LKPPLEMARL 250 260 270 280 290 300 FRRHPQALAE TLRFAEELTF SLSDLQYNYP DEPTESGLGP QAELERLARE GAARRYPSGV 310 320 330 340 350 360 PHSVIKRIEE ELALIERLNY ARYFLTVYDI VKYARSQDIL CQGRGSAANS IVCFCIGITE 370 380 390 400 410 420 VGPDRIDTLF ERFISEERNE PPDIDVDFEH EKRETVIQYI YEKYSSKRTA LAAAVISYRG 430 440 450 460 470 480 RSALREVSKA MGLSEDVRAS LSGSIWGWST SEVGEKEARA GGLDRSDPTS RHVMERANEI 490 500 510 520 530 540 MGFPRHLSQH VGGFVITKDR LDEIVPIVKT AMDERKMVEW DKDDLDAVKI LKVDVLALGM 550 560 570 580 590 600 LTCLQRAFIL LTDHYPKARD PYGQPYVLAS LPPEDKRVYD MIGRADTLGV FQIESRAQMS 610 620 630 640 650 660 MLPRLKPKDF YDLVIEVAIV RPGPIQGDMV HPYLRRRQGK EKPEYQKPEL EAILSKTLGV 670 680 690 700 710 720 PLFQEQAMKI AIVAGGFRPG EADELRRAMA TFKRTGTIGN YRQRMIDGMV GKGYTKDFAE 730 740 750 760 770 780 RCFKQIEGFG EYGFPESHAA SFALLVYASC WFKTFYPDVF CAAILNSQPM GFYQPAQLVR 790 800 810 820 830 840 DARDHGVDIR EVDVNCSAWD CTLEKVPFDP ARILPRHADM RGVIETNHAV RLGFRQIKGL 850 860 870 880 890 900 SKERMEDFAK RRGSGYETVR DVWLRSGLDV DEIERLAQAD AFRSIGLDRR AALWEVRALG 910 920 930 940 950 960 AKSAAEKLPL FDQPALRLRE LEPETKLPKM PLGEHVIHDY RSLGLSLKAH PVAFLRERLD 970 980 990 1000 1010 1020 RAGVTPNANL PSQRDGRRVS VAGLVLVRQR PGKGNAIFLT LEDDKAVANV IFWERTFTRF 1030 1040 1050 1060 1070 1080 RPIVMGARFV KVSGKLQSES GVIHIVAEKI EDLTPWLTVL LEKVSGAGAP GPQPMGYAKE 1090 1100 1110 VGSDRRSRPE IGNAPARQDL ATLSEEAEQV MPKGRNFQ

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB51086.1.
RefSeq	NP_105300.1.
3D structure databases
SMR	Q98E34. Positions 7-898.
ModBase	Search...
Genome annotation databases
GeneID	1227961.
GenomeReviews	Gene locus mlr4428 in contig BA000012_GR.
KEGG	mlo:mlr4428.
NMPDR	fig|266835.1.peg.3404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	LATNRNG.
Enzyme and pathway databases
BRENDA	2.7.7.7. 3315.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_RHILO

Accession

Primary (citable) accession number: Q98E34

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)