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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (mll4988)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK), Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ShikimateUniRule annotation1
Active sitei69Proton acceptorUniRule annotation1
Binding sitei90ShikimateUniRule annotation1
Binding sitei105ShikimateUniRule annotation1
Binding sitei219NADP; via carbonyl oxygenUniRule annotation1
Binding sitei221ShikimateUniRule annotation1
Binding sitei242NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi130 – 134NADPUniRule annotation5
Nucleotide bindingi154 – 159NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciMLOT266835:G1G20-3552-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:mlr4492
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001360261 – 280Shikimate dehydrogenase (NADP(+))Add BLAST280

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi266835.mlr4492

Structurei

3D structure databases

ProteinModelPortaliQ98DY3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 20Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237876
KOiK00014
OMAiIPHKERA
OrthoDBiPOG091H02DY

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

Q98DY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEATKKAFV TGHPIKHSRS PKIHGHWLAQ HGIDGSYEAI DVAPQDFAEF
60 70 80 90 100
IAALQANGFR GGNVTIPHKE AAFAVAQRRD QAAEEIGAVN TLWFEDGVLR
110 120 130 140 150
GGNTDGHGFA ANLDDCAPGW ANTGPAVVLG AGGASRAVIQ ALKQRGFSDI
160 170 180 190 200
RIVNRTLARA QELRDRFGAG VSAHGTAATD ELLADAGLLV NTTALGMVGN
210 220 230 240 250
EGLAADPALL PDHAIVTDLV YVPLETPLLA AARARGLKTV DGLGMLLNQA
260 270 280
VPGFEHWFGV RPQVTAELRA LIVADLVPKP
Length:280
Mass (Da):29,401
Last modified:October 1, 2001 - v1
Checksum:i603EDD25B2C28954
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA Translation: BAB51137.1
RefSeqiWP_010912479.1, NC_002678.2

Genome annotation databases

EnsemblBacteriaiBAB51137; BAB51137; BAB51137
KEGGimlo:mlr4492
PATRICifig|266835.9.peg.3550

Similar proteinsi

Entry informationi

Entry nameiAROE_RHILO
AccessioniPrimary (citable) accession number: Q98DY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 1, 2001
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health