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Protein

Malate synthase G

Gene

glcB

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei274Acetyl-CoAUniRule annotation1
Binding sitei311Acetyl-CoAUniRule annotation1
Active sitei338Proton acceptorUniRule annotation1
Binding sitei338GlyoxylateUniRule annotation1
Metal bindingi430MagnesiumUniRule annotation1
Binding sitei430GlyoxylateUniRule annotation1
Metal bindingi458MagnesiumUniRule annotation1
Binding sitei539Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei629Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMLOT266835:G1G20-3684-MONOMER
UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:mlr4664
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001668961 – 721Malate synthase GAdd BLAST721

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei615Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266835.mlr4664

Structurei

3D structure databases

ProteinModelPortaliQ98DK4
SMRiQ98DK4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125Acetyl-CoA bindingUniRule annotation2
Regioni455 – 458Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0

Family and domain databases

CDDicd00728 malate_synt_G, 1 hit
Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

Q98DK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRIEIAGL RIAGELHDFV AGEALPGTGI AADAFWSGFS AIVHDLAPKN
60 70 80 90 100
RALLKKRDAM QERLDGWYRD NGAPVDMEVY KSFLKEIGYL VPEGPAFSVS
110 120 130 140 150
TDNVDPEIAV VAGPQLVVPV MNARYALNAA NARWGSLYDA LYGTDAIPET
160 170 180 190 200
GGAEKGKGFN PARGAKVIAW AKDFLDQSVP LTSGKWAGVN GLSVAHGALK
210 220 230 240 250
LGAGAGGTTL ADPRQFAGYR GDAANPDAVL LVKNGLHIEI VVDRNNQIGR
260 270 280 290 300
TDPAGIADVI LESALTTIQD CEDSVAAVDA QDKVVVYRNW LGLMKGDLAE
310 320 330 340 350
EISKAGRSFV RKLNADRAYT APAGGQITVP GRSLMLVRNV GHLMTNPAIL
360 370 380 390 400
DRDGNEVPEG IMDAALTALI ALHDVGPEGR RANSRAGSMY VVKPKMHGPE
410 420 430 440 450
EVAFAVEIFD RVEALLGMPR NTIKMGIMDE ERRTTVNLKE AIRAARERVV
460 470 480 490 500
FINTGFLDRT GDEIHTSMEA GPMIRKGDMK QAAWISAYEA WNVDTGLECG
510 520 530 540 550
LAGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS PTAATLHATH
560 570 580 590 600
YHKVDVHAVQ AALKSRPKAK LDDILSVPVA VRPNWTPDEI QRELDNNAQG
610 620 630 640 650
ILGYVVRWID QGVGCSKVPD INDVGLMEDR ATLRISSQHI ANWLRHKVCS
660 670 680 690 700
EIQVRDSLQR MAAIVDRQNV GDPLYRPMAP DFDKSIAFQA ACDLVFKGTS
710 720
QPNGYTEPVL HARRLELKAQ R
Length:721
Mass (Da):78,059
Last modified:October 1, 2001 - v1
Checksum:i65376311A7E1BFDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA Translation: BAB51267.1
RefSeqiWP_010912609.1, NC_002678.2

Genome annotation databases

EnsemblBacteriaiBAB51267; BAB51267; BAB51267
KEGGimlo:mlr4664
PATRICifig|266835.9.peg.3688

Similar proteinsi

Entry informationi

Entry nameiMASZ_RHILO
AccessioniPrimary (citable) accession number: Q98DK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2001
Last modified: April 25, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health