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Protein

Malate synthase G

Gene

glcB

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei274 – 2741Acetyl-CoAUniRule annotation
Binding sitei311 – 3111Acetyl-CoAUniRule annotation
Active sitei338 – 3381Proton acceptorUniRule annotation
Binding sitei338 – 3381GlyoxylateUniRule annotation
Metal bindingi430 – 4301MagnesiumUniRule annotation
Binding sitei430 – 4301GlyoxylateUniRule annotation
Metal bindingi458 – 4581MagnesiumUniRule annotation
Binding sitei539 – 5391Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei629 – 6291Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMLOT266835:GJ9L-3627-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:mlr4664
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
ProteomesiUP000000552 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Malate synthase GPRO_0000166896Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei615 – 6151Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266835.mlr4664.

Structurei

3D structure databases

ProteinModelPortaliQ98DK4.
SMRiQ98DK4. Positions 2-720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
Regioni455 – 4584Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q98DK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRIEIAGL RIAGELHDFV AGEALPGTGI AADAFWSGFS AIVHDLAPKN
60 70 80 90 100
RALLKKRDAM QERLDGWYRD NGAPVDMEVY KSFLKEIGYL VPEGPAFSVS
110 120 130 140 150
TDNVDPEIAV VAGPQLVVPV MNARYALNAA NARWGSLYDA LYGTDAIPET
160 170 180 190 200
GGAEKGKGFN PARGAKVIAW AKDFLDQSVP LTSGKWAGVN GLSVAHGALK
210 220 230 240 250
LGAGAGGTTL ADPRQFAGYR GDAANPDAVL LVKNGLHIEI VVDRNNQIGR
260 270 280 290 300
TDPAGIADVI LESALTTIQD CEDSVAAVDA QDKVVVYRNW LGLMKGDLAE
310 320 330 340 350
EISKAGRSFV RKLNADRAYT APAGGQITVP GRSLMLVRNV GHLMTNPAIL
360 370 380 390 400
DRDGNEVPEG IMDAALTALI ALHDVGPEGR RANSRAGSMY VVKPKMHGPE
410 420 430 440 450
EVAFAVEIFD RVEALLGMPR NTIKMGIMDE ERRTTVNLKE AIRAARERVV
460 470 480 490 500
FINTGFLDRT GDEIHTSMEA GPMIRKGDMK QAAWISAYEA WNVDTGLECG
510 520 530 540 550
LAGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS PTAATLHATH
560 570 580 590 600
YHKVDVHAVQ AALKSRPKAK LDDILSVPVA VRPNWTPDEI QRELDNNAQG
610 620 630 640 650
ILGYVVRWID QGVGCSKVPD INDVGLMEDR ATLRISSQHI ANWLRHKVCS
660 670 680 690 700
EIQVRDSLQR MAAIVDRQNV GDPLYRPMAP DFDKSIAFQA ACDLVFKGTS
710 720
QPNGYTEPVL HARRLELKAQ R
Length:721
Mass (Da):78,059
Last modified:October 1, 2001 - v1
Checksum:i65376311A7E1BFDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB51267.1.
RefSeqiNP_105481.1. NC_002678.2.
WP_010912609.1. NC_002678.2.

Genome annotation databases

EnsemblBacteriaiBAB51267; BAB51267; BAB51267.
GeneIDi1228142.
KEGGimlo:mlr4664.
PATRICi22481607. VBIMesLot2464_3688.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB51267.1.
RefSeqiNP_105481.1. NC_002678.2.
WP_010912609.1. NC_002678.2.

3D structure databases

ProteinModelPortaliQ98DK4.
SMRiQ98DK4. Positions 2-720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266835.mlr4664.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB51267; BAB51267; BAB51267.
GeneIDi1228142.
KEGGimlo:mlr4664.
PATRICi22481607. VBIMesLot2464_3688.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciMLOT266835:GJ9L-3627-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MAFF303099.

Entry informationi

Entry nameiMASZ_RHILO
AccessioniPrimary (citable) accession number: Q98DK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2001
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.