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Q98DK4

- MASZ_RHILO

UniProt

Q98DK4 - MASZ_RHILO

Protein

Malate synthase G

Gene

glcB

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei274 – 2741Acetyl-CoAUniRule annotation
    Binding sitei311 – 3111Acetyl-CoAUniRule annotation
    Active sitei338 – 3381Proton acceptorUniRule annotation
    Binding sitei338 – 3381GlyoxylateUniRule annotation
    Metal bindingi430 – 4301MagnesiumUniRule annotation
    Binding sitei430 – 4301GlyoxylateUniRule annotation
    Metal bindingi458 – 4581MagnesiumUniRule annotation
    Binding sitei539 – 5391Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei629 – 6291Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMLOT266835:GJ9L-3627-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:mlr4664
    OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
    Taxonomic identifieri266835 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
    ProteomesiUP000000552: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 721721Malate synthase GPRO_0000166896Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei615 – 6151Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi266835.mlr4664.

    Structurei

    3D structure databases

    ProteinModelPortaliQ98DK4.
    SMRiQ98DK4. Positions 2-720.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
    Regioni455 – 4584Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q98DK4-1 [UniParc]FASTAAdd to Basket

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    MTDRIEIAGL RIAGELHDFV AGEALPGTGI AADAFWSGFS AIVHDLAPKN    50
    RALLKKRDAM QERLDGWYRD NGAPVDMEVY KSFLKEIGYL VPEGPAFSVS 100
    TDNVDPEIAV VAGPQLVVPV MNARYALNAA NARWGSLYDA LYGTDAIPET 150
    GGAEKGKGFN PARGAKVIAW AKDFLDQSVP LTSGKWAGVN GLSVAHGALK 200
    LGAGAGGTTL ADPRQFAGYR GDAANPDAVL LVKNGLHIEI VVDRNNQIGR 250
    TDPAGIADVI LESALTTIQD CEDSVAAVDA QDKVVVYRNW LGLMKGDLAE 300
    EISKAGRSFV RKLNADRAYT APAGGQITVP GRSLMLVRNV GHLMTNPAIL 350
    DRDGNEVPEG IMDAALTALI ALHDVGPEGR RANSRAGSMY VVKPKMHGPE 400
    EVAFAVEIFD RVEALLGMPR NTIKMGIMDE ERRTTVNLKE AIRAARERVV 450
    FINTGFLDRT GDEIHTSMEA GPMIRKGDMK QAAWISAYEA WNVDTGLECG 500
    LAGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS PTAATLHATH 550
    YHKVDVHAVQ AALKSRPKAK LDDILSVPVA VRPNWTPDEI QRELDNNAQG 600
    ILGYVVRWID QGVGCSKVPD INDVGLMEDR ATLRISSQHI ANWLRHKVCS 650
    EIQVRDSLQR MAAIVDRQNV GDPLYRPMAP DFDKSIAFQA ACDLVFKGTS 700
    QPNGYTEPVL HARRLELKAQ R 721
    Length:721
    Mass (Da):78,059
    Last modified:October 1, 2001 - v1
    Checksum:i65376311A7E1BFDF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000012 Genomic DNA. Translation: BAB51267.1.
    RefSeqiNP_105481.1. NC_002678.2.
    WP_010912609.1. NC_002678.2.

    Genome annotation databases

    EnsemblBacteriaiBAB51267; BAB51267; BAB51267.
    GeneIDi1228142.
    KEGGimlo:mlr4664.
    PATRICi22481607. VBIMesLot2464_3688.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000012 Genomic DNA. Translation: BAB51267.1 .
    RefSeqi NP_105481.1. NC_002678.2.
    WP_010912609.1. NC_002678.2.

    3D structure databases

    ProteinModelPortali Q98DK4.
    SMRi Q98DK4. Positions 2-720.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266835.mlr4664.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB51267 ; BAB51267 ; BAB51267 .
    GeneIDi 1228142.
    KEGGi mlo:mlr4664.
    PATRICi 22481607. VBIMesLot2464_3688.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci MLOT266835:GJ9L-3627-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MAFF303099.

    Entry informationi

    Entry nameiMASZ_RHILO
    AccessioniPrimary (citable) accession number: Q98DK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3