Q98D72 (PH4H_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine-4-hydroxylase
Short name=PAH
EC=1.14.16.1

Alternative name(s):
Phe-4-monooxygenase

Gene names

Name:	phhA
Ordered Locus Names:	mlr4831

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-phenylalanine + tetrahydrobiopterin + O2 = L-tyrosine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Binds 1 Fe2+ ion By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 1/6.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro phenylalanine 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Phenylalanine-4-hydroxylase		PRO_0000205557
Sites
	Metal binding	135	1	Iron Potential
	Metal binding	140	1	Iron Potential
	Metal binding	181	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	Q98D72 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: BC29D255534BC215	FASTA	275	31,348
	10 20 30 40 50 60 MSVAEYARDC AAQGLRGDYS VCRADFTVAQ DYDYSDEEQA VWRTLCDRQT KLTRKLAHHS 70 80 90 100 110 120 YLDGVEKLGL LDRIPDFEDV STKLRKLTGW EIIAVPGLIP AAPFFDHLAN RRFPVTNWLR 130 140 150 160 170 180 TRQELDYIVE PDMFHDFFGH VPVLSQPVFA DFMQMYGKKA GDIIALGGDE MITRLYWYTA 190 200 210 220 230 240 EYGLVQEAGQ PLKAFGAGLM SSFTELQFAV EGKDAHHVPF DLETVMRTGY EIDKFQRAYF 250 260 270 VLPSFDALRD AFQTADFEAI VARRKDQKAL DPATV

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB51399.1.
RefSeq	NP_105613.1. NC_002678.2.
3D structure databases
ProteinModelPortal	Q98D72.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1228274.
GenomeReviews	Gene locus mlr4831 in contig BA000012_GR.
KEGG	mlo:mlr4831.
NMPDR	fig|266835.1.peg.3717.
PATRIC	22481864. VBIMesLot2464_3815.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG403840.
OMA	YKIDSYQ.
ProtClustDB	PRK11913.
Family and domain databases
InterPro	IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005960. Phe-4-hydroxylase_mono. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
KO	K00500.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. [Graphical view]
PRINTS	PR00372. FYWHYDRXLASE.
SUPFAM	SSF56534. Aaa_hydroxylase. 1 hit.
TIGRFAMs	TIGR01267. Phe4hydrox_mono. 1 hit.
PROSITE	PS00367. BH4_AAA_HYDROXYL_1. 1 hit. PS51410. BH4_AAA_HYDROXYL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PH4H_RHILO

Accession

Primary (citable) accession number: Q98D72

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program