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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61SubstrateUniRule annotation1
Binding sitei195SubstrateUniRule annotation1
Metal bindingi201ManganeseUniRule annotation1
Binding sitei201ATPUniRule annotation1
Binding sitei201SubstrateUniRule annotation1
Metal bindingi220Manganese; via tele nitrogenUniRule annotation1
Binding sitei220ATPUniRule annotation1
Metal bindingi257ManganeseUniRule annotation1
Binding sitei285ATPUniRule annotation1
Binding sitei322ATPUniRule annotation1
Binding sitei322SubstrateUniRule annotation1
Binding sitei447ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi236 – 244ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:mlr5096
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002038351 – 536Phosphoenolpyruvate carboxykinase [ATP]Add BLAST536

Interactioni

Protein-protein interaction databases

STRINGi266835.mlr5096.

Structurei

3D structure databases

ProteinModelPortaliQ98CL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q98CL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVGKRNPA CPIDRIGLKT TGMVRYNFGA AALYEEAIRR GEARLTAHGA
60 70 80 90 100
LVAETGQHTG RSPKDKFVVR DAATEPEVWW DNNKAISPAQ FETLFADFLA
110 120 130 140 150
HAANKDLYVQ DLVGGADAEL KLPTRVITEF AWHSLFIRNL LIRPDKAELG
160 170 180 190 200
QFVPEMTIID LPSFRADPAR HGSRTETVIA VDLTRQIVLI GGTSYAGEMK
210 220 230 240 250
KSVFTMLNYI LPQKGVMPMH CSANEGPAGD AAVFFGLSGT GKTTLSADPS
260 270 280 290 300
RTLIGDDEHG WGPHGIFNFE GGCYAKTIKL SAEAEPEIFA TTQRFGTVLE
310 320 330 340 350
NVVLDADGVP DFNDGRLTEN TRCAYPLDFI PNASKTGRAS HPKNIIMLTA
360 370 380 390 400
DAFGVMPPIA RLTPAQAMYH FLSGYTAKVA GTEKGVTEPE ATFSTCFGAP
410 420 430 440 450
FMPRHPSEYG NLLRELIASH GADCWLVNTG WTGGAYGTGK RMPIKATRAL
460 470 480 490 500
LTAALDGSLK TGEFRTDANF GFEVPVAVPG VDSAILDPRS TWADKPAYDR
510 520 530
QAARLVGMFA VNFEKFEPHV DATVMGAAPR MQEAAE
Length:536
Mass (Da):58,036
Last modified:September 26, 2001 - v1
Checksum:i8A3E5D3C7E290743
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB51604.1.
RefSeqiWP_010912943.1. NC_002678.2.

Genome annotation databases

EnsemblBacteriaiBAB51604; BAB51604; BAB51604.
GeneIDi1228479.
KEGGimlo:mlr5096.
PATRICi22482288. VBIMesLot2464_4026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB51604.1.
RefSeqiWP_010912943.1. NC_002678.2.

3D structure databases

ProteinModelPortaliQ98CL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266835.mlr5096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB51604; BAB51604; BAB51604.
GeneIDi1228479.
KEGGimlo:mlr5096.
PATRICi22482288. VBIMesLot2464_4026.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKA_RHILO
AccessioniPrimary (citable) accession number: Q98CL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.