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Q98C31 (SYW_RHILO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:mll5317
OrganismRhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]
Taxonomic identifier266835 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136666

Regions

Motif14 – 229"HIGH" region HAMAP-Rule MF_00140
Motif217 – 2215"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q98C31 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: AA8CD99496A7CB7E

FASTA35538,760
        10         20         30         40         50         60 
MSAFKPLVFS GVQPTGNLHL GNYLGAIKKF VALQETSDCI YCVVDLHSLT AQLVHEDLAD 

        70         80         90        100        110        120 
QTRSITAAFL ASGIDPKKHI VFNQSRVMQH AELAWIFNCV ARIGWMNKMT QFKDKAGKDR 

       130        140        150        160        170        180 
ENASLGLLAY PSLMAADILL YRATHVPVGE DQKQHLELTR DIAQKFNNDF SDRIARLGVG 

       190        200        210        220        230        240 
VEMQVGEETV NGFFPITEPV IGGPAARIMS LRDGSKKMSK SDPSDLSRIN LTDDADTISK 

       250        260        270        280        290        300 
KIRKAKTDPE ALPSEVSGLE SRPEAENLVG IYAGLAEMSK ADVLKEFGGQ QFSVFKPALA 

       310        320        330        340        350 
DLAVEKLAPI AGEMRRIEGD RAYVDAVLRD GGERAGVLAE TTMKTVRDII GLLQG 

« Hide

References

[1]"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti."
Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. expand/collapse author list , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S.
DNA Res. 7:331-338(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MAFF303099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000012 Genomic DNA. Translation: BAB51790.1.
RefSeqNP_106004.1. NC_002678.2.

3D structure databases

ProteinModelPortalQ98C31.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266835.mll5317.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB51790; BAB51790; BAB51790.
GeneID1228665.
KEGGmlo:mll5317.
PATRIC22482670. VBIMesLot2464_4216.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059940.
KOK01867.
OMAIDPKKHI.
OrthoDBEOG686NJQ.

Enzyme and pathway databases

BioCycMLOT266835:GJ9L-4153-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_RHILO
AccessionPrimary (citable) accession number: Q98C31
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries