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Q98C27

- GLND_RHILO

UniProt

Q98C27 - GLND_RHILO

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciMLOT266835:GJ9L-4157-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:mll5321
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
ProteomesiUP000000552: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192759Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266835.mll5321.

Structurei

3D structure databases

ProteinModelPortaliQ98C27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini497 – 596100HDUniRule annotationAdd
BLAST
Domaini737 – 81882ACT 1UniRule annotationAdd
BLAST
Domaini848 – 92275ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 379379UridylyltransferaseAdd
BLAST
Regioni380 – 736357Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q98C27-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKISLKLDE LIDGEALRRE MTALTTATAG DGSGAAARAG VLQLLKARLA
60 70 80 90 100
EGRKIAEAML KEDGGGNACA ERLSHLMDEL IRALYDFAAT HVYRVKNRSS
110 120 130 140 150
AERMAVVAVG GYGRGTLAPG SDIDLLFLLP YKQTPWGEQT VEYMLYMLWD
160 170 180 190 200
LGLKVGHATR NIDECLRLSR TDITIRTSIL EARFLWGERK LYDELMLRFD
210 220 230 240 250
HEVVRTTGPE YVQAKLAERD ERHAKAGESR YLVEPNVKDG KGGLRDLQTL
260 270 280 290 300
FWIGKYFYRV RTGEELVEKG VFTEAEYREF QKAEDFLWAV RCHMHFLTGK
310 320 330 340 350
AEERLHFDIQ REIAERLGYT THPGLSAVER FMKHYFLVAK DVGDLTRIFC
360 370 380 390 400
AALEEEQAKH VPGFNRIFLT FQRRKRKLAG TSDFIVDNHR INIADDQVFE
410 420 430 440 450
RDPVNLLRLF WFADKHGLEF HPDALKLLTR SLGLVNKSLR RDEEANRLFL
460 470 480 490 500
DILTSDRNAE LNLRRMNEAG LLGRLIPDFG KIVAMMQFSM YHHYTVDEHL
510 520 530 540 550
IRCIGVLAEI ERGDGEKVHP LSHSLMPGLK KSREALYVAV LLHDIAKGRP
560 570 580 590 600
EDHSEAGARI ARRICPHMGL SAADTETVAW LVENHLAMSM TAQTRDLNDR
610 620 630 640 650
KTIEDFASIV QSVERLKLLL VLTVCDIRGV GPGVWNGWKG QLLRTLYYET
660 670 680 690 700
ELLLTGGFSE VSRAQRTAAA RERLAEALAD WPDKDRKRYV GLHYENYLLT
710 720 730 740 750
VDLPDQLRHA EFVREADAAG KKLATMVKTH QFEAVTEITV LAQDHPRLLS
760 770 780 790 800
VIAGACVGAG GNIVDAQIFT TADGRALDTI LISREFDRDE DERRRAERVG
810 820 830 840 850
RLIEDVLSGK SWLPEMIEKR TKPKRGAKVF KIPPRAEIRN TLSNRFSVIE
860 870 880 890 900
VEGLDRPGLL SEITGTLSDL SLDIASAHIT TFGEKVIDTF YVTDLTGQKI
910 920 930
DSPARIATIR NRLMATLEGI APERGGKAKA AAE
Length:933
Mass (Da):105,280
Last modified:October 1, 2001 - v1
Checksum:i79BF065DAC796FF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB51794.1.
RefSeqiNP_106008.1. NC_002678.2.

Genome annotation databases

EnsemblBacteriaiBAB51794; BAB51794; BAB51794.
GeneIDi1228669.
KEGGimlo:mll5321.
PATRICi22482678. VBIMesLot2464_4220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB51794.1 .
RefSeqi NP_106008.1. NC_002678.2.

3D structure databases

ProteinModelPortali Q98C27.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 266835.mll5321.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB51794 ; BAB51794 ; BAB51794 .
GeneIDi 1228669.
KEGGi mlo:mll5321.
PATRICi 22482678. VBIMesLot2464_4220.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci MLOT266835:GJ9L-4157-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MAFF303099.

Entry informationi

Entry nameiGLND_RHILO
AccessioniPrimary (citable) accession number: Q98C27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: October 1, 2001
Last modified: November 26, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3