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Q98C27

- GLND_RHILO

UniProt

Q98C27 - GLND_RHILO

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, mll5321
Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciMLOT266835:GJ9L-4157-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:mll5321
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
ProteomesiUP000000552: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_0000192759Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266835.mll5321.

Structurei

3D structure databases

ProteinModelPortaliQ98C27.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini497 – 596100HD
Add
BLAST
Domaini737 – 81882ACT 1
Add
BLAST
Domaini848 – 92275ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 379379UridylyltransferaseUniRule annotation
Add
BLAST
Regioni380 – 736357Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q98C27-1 [UniParc]FASTAAdd to Basket

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MAKISLKLDE LIDGEALRRE MTALTTATAG DGSGAAARAG VLQLLKARLA    50
EGRKIAEAML KEDGGGNACA ERLSHLMDEL IRALYDFAAT HVYRVKNRSS 100
AERMAVVAVG GYGRGTLAPG SDIDLLFLLP YKQTPWGEQT VEYMLYMLWD 150
LGLKVGHATR NIDECLRLSR TDITIRTSIL EARFLWGERK LYDELMLRFD 200
HEVVRTTGPE YVQAKLAERD ERHAKAGESR YLVEPNVKDG KGGLRDLQTL 250
FWIGKYFYRV RTGEELVEKG VFTEAEYREF QKAEDFLWAV RCHMHFLTGK 300
AEERLHFDIQ REIAERLGYT THPGLSAVER FMKHYFLVAK DVGDLTRIFC 350
AALEEEQAKH VPGFNRIFLT FQRRKRKLAG TSDFIVDNHR INIADDQVFE 400
RDPVNLLRLF WFADKHGLEF HPDALKLLTR SLGLVNKSLR RDEEANRLFL 450
DILTSDRNAE LNLRRMNEAG LLGRLIPDFG KIVAMMQFSM YHHYTVDEHL 500
IRCIGVLAEI ERGDGEKVHP LSHSLMPGLK KSREALYVAV LLHDIAKGRP 550
EDHSEAGARI ARRICPHMGL SAADTETVAW LVENHLAMSM TAQTRDLNDR 600
KTIEDFASIV QSVERLKLLL VLTVCDIRGV GPGVWNGWKG QLLRTLYYET 650
ELLLTGGFSE VSRAQRTAAA RERLAEALAD WPDKDRKRYV GLHYENYLLT 700
VDLPDQLRHA EFVREADAAG KKLATMVKTH QFEAVTEITV LAQDHPRLLS 750
VIAGACVGAG GNIVDAQIFT TADGRALDTI LISREFDRDE DERRRAERVG 800
RLIEDVLSGK SWLPEMIEKR TKPKRGAKVF KIPPRAEIRN TLSNRFSVIE 850
VEGLDRPGLL SEITGTLSDL SLDIASAHIT TFGEKVIDTF YVTDLTGQKI 900
DSPARIATIR NRLMATLEGI APERGGKAKA AAE 933
Length:933
Mass (Da):105,280
Last modified:October 1, 2001 - v1
Checksum:i79BF065DAC796FF8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000012 Genomic DNA. Translation: BAB51794.1.
RefSeqiNP_106008.1. NC_002678.2.
WP_010913133.1. NC_002678.2.

Genome annotation databases

EnsemblBacteriaiBAB51794; BAB51794; BAB51794.
GeneIDi1228669.
KEGGimlo:mll5321.
PATRICi22482678. VBIMesLot2464_4220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000012 Genomic DNA. Translation: BAB51794.1 .
RefSeqi NP_106008.1. NC_002678.2.
WP_010913133.1. NC_002678.2.

3D structure databases

ProteinModelPortali Q98C27.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 266835.mll5321.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB51794 ; BAB51794 ; BAB51794 .
GeneIDi 1228669.
KEGGi mlo:mll5321.
PATRICi 22482678. VBIMesLot2464_4220.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci MLOT266835:GJ9L-4157-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MAFF303099.

Entry informationi

Entry nameiGLND_RHILO
AccessioniPrimary (citable) accession number: Q98C27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: October 1, 2001
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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