Reviewed, UniProtKB/Swiss-Prot Q98BZ8 (ILVD1_RHILO)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 1
      Short name=DAD 1
    EC=4.2.1.9

Gene names

Name:	ilvD1
Synonyms:	ilvD
Ordered Locus Names:	mlr5361

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000103496
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q98BZ8-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 963C8B913FDAB49A	FASTA	614	65,367
	10 20 30 40 50 60 MPAYRSRTTT HGRNMAGARG LWRATGMKDS DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEKAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRELI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MASLRLNIPT VFVSGGPMEA GKVVLAGKTQ ALDLVDAMVA AADDKISDED 190 200 210 220 230 240 VKVIERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS TLATHADRKR LFVEAGHLIV 250 260 270 280 290 300 DLAQRYYEQD DETALPRSIA SKGAFENAMT LDIAMGGSTN TVLHILAAAH EGEVDFTMED 310 320 330 340 350 360 IDRLSRRVPV LCKVAPAKSD VHMEDVHRAG GIMAILGQLD NAGLINRDLP TVHTSSLGEA 370 380 390 400 410 420 LDHWDISRTS SQNVRDFFLA APGGVPTQVA FSQDRRWDEL DLDREKGVIR SAETPFSKDG 430 440 450 460 470 480 GLAVLKGNLA LDGCIVKTAG VDESILKFTG PARVFESQDA SVKAILSNEI KAGDVVVIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGLGKACAL VTDGRFSGGT SGLSIGHASP EAAEGGTIGL 550 560 570 580 590 600 VQEGDTIEID IPNRTIRLAV SDAELDSRRA AMEAKGALAW KPEEKRKRKV TTALRAYAAF 610 ATSADKGAVR HVPE

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000012 Genomic DNA. Translation: BAB51823.1.
RefSeq	NP_106037.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1228698.
GenomeReviews	Gene locus mlr5361 in contig BA000012_GR.
KEGG	mlo:mlr5361.
NMPDR	fig|266835.1.peg.4141.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q98BZ8.
OMA	SRKVPCL.
Enzyme and pathway databases
BRENDA	4.2.1.9. 3315.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_RHILO

Accession

Primary (citable) accession number: Q98BZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)