Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64S-adenosyl-L-methionineUniRule annotation1
Binding sitei89S-adenosyl-L-methionineUniRule annotation1
Binding sitei116S-adenosyl-L-methionineUniRule annotation1
Active sitei138By similarity1
Binding sitei138S-adenosyl-L-methionineUniRule annotation1
Binding sitei142SubstrateUniRule annotation1
Binding sitei174SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMLOT266835:G1G20-4409-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:mlr5547
OrganismiMesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713801 – 233tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST233

Interactioni

Protein-protein interaction databases

STRINGi266835.mlr5547

Structurei

3D structure databases

ProteinModelPortaliQ98BJ3
SMRiQ98BJ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 215Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073969
KOiK03439
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q98BJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPQDRPSRT TEAFFGRRRG KPVRPQQAAA LESGLDAYRL DLTADAPSDL
60 70 80 90 100
RTLFETEVSA VRLEIGFGGG EHLLHRAIEA PTTGFIGVEP FVNGMAKMMM
110 120 130 140 150
AVRARPLANL RVHDDDATRL LDWLPPASLG GIDLLYPDPW PKKKHWKRRF
160 170 180 190 200
VSPVNLERFA RVLKPGAKFR FASDIDTYVN WTLLHCRAHG AFAWQAAEAA
210 220 230
DWHRPYEGWP GTRYEAKAIR EGRRPAYLTF VRK
Length:233
Mass (Da):26,434
Last modified:May 29, 2007 - v2
Checksum:i5F0E2631F2080546
GO

Sequence cautioni

The sequence BAB51979 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA Translation: BAB51979.1 Different initiation.
RefSeqiWP_044551168.1, NC_002678.2

Genome annotation databases

EnsemblBacteriaiBAB51979; BAB51979; BAB51979
KEGGimlo:mlr5547
PATRICifig|266835.9.peg.4411

Similar proteinsi

Entry informationi

Entry nameiTRMB_RHILO
AccessioniPrimary (citable) accession number: Q98BJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 79 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health