Reviewed, UniProtKB/Swiss-Prot Q98AN7 (PYRE1_RHILO)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase 1
      Short name=OPRT 1
      Short name=OPRTase 1
    EC=2.4.2.10

Gene names

Name:	pyrE1
Ordered Locus Names:	mll5916

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Orotate phosphoribosyltransferase 1 HAMAP MF_01208		PRO_0000110729
Regions
	Region	138 – 146	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	108	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	109	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	112	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	114	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	142	1	Orotate By similarity
	Binding site	170	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98AN7-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: C51164769A4D38FC	FASTA	198	21,501
	10 20 30 40 50 60 MAALAKFRPV DSGQHDKDEL REIVRERSFR FGRYTLSSGI ESDIYFNMKP TMMVARGAQL 70 80 90 100 110 120 AALEFLKIVE QVKAEYVGGL EMGAVPVIGS MAAVSSMMNK PINTIFVRKV PKAHGTRDVI 130 140 150 160 170 180 EGLGPKEDLE GKVVLIVDDV ATSGKSILKA IEEVRRVGGI VGDAACLVDR DEGATALLAQ 190 HGVTLHSVLH ASEFVERH

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000012 Genomic DNA. Translation: BAB52285.1.
RefSeq	NP_106499.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1229158.
GenomeReviews	Gene locus mll5916 in contig BA000012_GR.
KEGG	mlo:mll5916.
NMPDR	fig|266835.1.peg.4602.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q98AN7.
OMA	EPKGHGT.
Enzyme and pathway databases
BRENDA	2.4.2.10. 3315.
Family and domain databases
HAMAP	MF_01208. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE1_RHILO

Accession

Primary (citable) accession number: Q98AN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)