Reviewed, UniProtKB/Swiss-Prot Q98A81 (GLYA2_RHILO)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	mlr6114

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000113647
Amino acid modifications
	Modified residue	234	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98A81-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 0DB6E5CAD67F3B61	FASTA	437	47,991
	10 20 30 40 50 60 MAEPGTYGRS SLVQVDCRVH ELLLRQRRQE RTMLKLIASE NFASSAVLEA TGSIFANKYA 70 80 90 100 110 120 EGYPGARYYA GNEIVDELET LAIERLKALF GSEHANVQPY SGSPANQAVY RALLSPRDKV 130 140 150 160 170 180 MGLPLPEGGH LTHGWSVNFS GTDYQRVPYG LHDKTQQIDY DRLRETARRE RPKLIWVGGT 190 200 210 220 230 240 SYPRVFDYAA MAEIALEANS YLVADIAHIS GLIVAGAHPN PVVHCDVVTS TSHKSIRGPR 250 260 270 280 290 300 GGFILSKNED RYQALYHSTS KHNLAKRIDR AVFPQLQGGP HMNTIAALAV ALQEAATPSF 310 320 330 340 350 360 RTYGHQIVKN AKALAEALLG RGYYLVTGGT DNHMLILDLR DRPLSGKAYA ERLARAGIIT 370 380 390 400 410 420 NFDMVPGDPR DPTVTSGIRL GSPAVTSMGM REAEMVQIAA FIDSVCRQPD DQEVHASVRR 430 DVADFCTAFD VPGISDR

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB52456.1.
RefSeq	NP_106670.1.
3D structure databases
SMR	Q98A81. Positions 15-432.
ModBase	Search...
Genome annotation databases
GeneID	1229325.
GenomeReviews	Gene locus mlr6114 in contig BA000012_GR.
KEGG	mlo:mlr6114.
NMPDR	fig|266835.1.peg.4771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG301263.
OMA	GGNEVID.
Enzyme and pathway databases
BRENDA	2.1.2.1. 3315.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_RHILO

Accession

Primary (citable) accession number: Q98A81

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	October 1, 2001
Last modified:	January 19, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)