Reviewed, UniProtKB/Swiss-Prot Q989S7 (PROB2_RHILO)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase 2
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase 2
      Short name=GK 2

Gene names

Name:	proB2
Ordered Locus Names:	mlr6298

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Glutamate 5-kinase 2 HAMAP MF_00456		PRO_0000109714

Sequences

	Sequence		Length	Mass (Da)
	Q989S7-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: C7E8BB7D23C4FA54	FASTA	284	30,683
	10 20 30 40 50 60 MQEKIRETCL ATKTVKLLSA RRLIVKIGSA VVADAETGEI RGPWLETLIK DVVRFFARGQ 70 80 90 100 110 120 QVIIVTSGAV AAGSRHFKQL DRSLRIEEKQ AAAAIGQIRL MIAYEQSLKR HGFGLGQVLL 130 140 150 160 170 180 TSADVDNQRC RLNARSAFQQ LLNVGAVPVI NENDATATPE VCLGDNDRLA ARVAQIAKAD 190 200 210 220 230 240 LLILLSDVDG LFTEDPHDNP LARMIPEVRR ITPEIEIMAS LSPARHGSGG MVTKLMAARI 250 260 270 280 AMEAGCNVVI AKGSKSYPLA AIENGAPSTW FIPPARETAT RGGR

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000012 Genomic DNA. Translation: BAB52617.1.
RefSeq	NP_106831.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1229486.
GenomeReviews	Gene locus mlr6298 in contig BA000012_GR.
KEGG	mlo:mlr6298.
NMPDR	fig|266835.1.peg.4932.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q989S7.
OMA	ELMHIYS.
Enzyme and pathway databases
BRENDA	2.7.2.11. 3315.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB2_RHILO

Accession

Primary (citable) accession number: Q989S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)