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Protein

Indian hedgehog protein

Gene

IHH

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi90Calcium 1By similarity1
Metal bindingi91Calcium 1By similarity1
Metal bindingi91Calcium 2By similarity1
Metal bindingi96Calcium 1By similarity1
Metal bindingi126Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi127Calcium 1By similarity1
Metal bindingi127Calcium 2By similarity1
Metal bindingi130Calcium 2By similarity1
Metal bindingi132Calcium 2By similarity1
Metal bindingi141ZincBy similarity1
Metal bindingi148ZincBy similarity1
Metal bindingi183ZincBy similarity1
Sitei244Involved in cholesterol transferBy similarity1
Sitei268Involved in auto-cleavageBy similarity1
Sitei271Essential for auto-cleavageBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cartilage development Source: UniProtKB
  • cell-cell signaling Source: InterPro
  • chondroblast differentiation Source: AgBase
  • chondrocyte proliferation Source: AgBase
  • embryonic digit morphogenesis Source: AgBase
  • negative regulation of chondrocyte differentiation Source: AgBase
  • negative regulation of chondrocyte hypertrophy Source: AgBase
  • positive regulation of alkaline phosphatase activity Source: AgBase
  • positive regulation of cell morphogenesis involved in differentiation Source: AgBase
  • positive regulation of gene expression Source: AgBase
  • positive regulation of hh target transcription factor activity Source: AgBase
  • positive regulation of osteoblast differentiation Source: AgBase
  • proteoglycan biosynthetic process Source: AgBase
  • regulation of chondrocyte differentiation involved in endochondral bone morphogenesis Source: AgBase
  • regulation of growth plate cartilage chondrocyte differentiation Source: AgBase
  • regulation of growth plate cartilage chondrocyte proliferation Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiC46.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Indian hedgehog protein
Short name:
IHH
Cleaved into the following 2 chains:
Gene namesi
Name:IHH
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Indian hedgehog protein N-product :
Indian hedgehog protein C-product :

GO - Cellular componenti

  • extracellular space Source: AgBase
  • intracellular Source: GOC
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001323524 – 408Indian hedgehog proteinAdd BLAST385
ChainiPRO_000001323624 – 198Indian hedgehog protein N-productAdd BLAST175
ChainiPRO_0000013237199 – 408Indian hedgehog protein C-productAdd BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi24N-palmitoyl cysteineBy similarity1
Lipidationi198Cholesterol glycine esterBy similarity1

Post-translational modificationi

The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).By similarity
Cholesterylation is required for N-product targeting to lipid rafts and multimerization.By similarity
N-palmitoylation is required for N-product multimerization and full activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei198 – 199Cleavage; by autolysisBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ98938.

Expressioni

Tissue specificityi

Expressed in developing midgut, lung and cartilage of developing long bones in the limb.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018493.

Structurei

3D structure databases

ProteinModelPortaliQ98938.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The indian hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain.By similarity

Sequence similaritiesi

Belongs to the hedgehog family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3638. Eukaryota.
ENOG410XQA3. LUCA.
GeneTreeiENSGT00390000001117.
HOGENOMiHOG000233428.
HOVERGENiHBG005480.
InParanoidiQ98938.
KOiK11989.
PhylomeDBiQ98938.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.30.1380.10. 1 hit.
InterProiIPR001657. Hedgehog.
IPR028992. Hedgehog/Intein_dom.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR033385. IHH.
[Graphical view]
PANTHERiPTHR11889:SF39. PTHR11889:SF39. 1 hit.
PfamiPF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view]
PIRSFiPIRSF009400. Peptidase_C46. 1 hit.
PRINTSiPR00632. SONICHHOG.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF55166. SSF55166. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q98938-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPARLLLLL SGCALLLAPA VRCCGPGRVV GSRRRPPRKL IPLAYKQFSP
60 70 80 90 100
NVPEKTLGAS GRYEGKIARN SERFKELTPN YNPDIIFKDE ENTGADRLMT
110 120 130 140 150
QRCKDRLNSL AISVMNQWPG VKLRVTEGWD EDGHHSEESL HYEGRAVDIT
160 170 180 190 200
TSDRDRNKYG MLARLAVEAG FDWVYYESKA HIHCSVKSEH SAAAKTGGCF
210 220 230 240 250
PGRALATLEN GARTPLWALR PGQRVLAMDG AGRPTYSDFL AFLDKEPRAL
260 270 280 290 300
TAFHVIETRQ PPRRLALTPT HLLFVADNAS APAAQFRPTF ASHVQPGHFV
310 320 330 340 350
LVAVGSGGLQ PAEVVGVRGR TDVGAYAPLT RHGTLVVDDV VASCFALVRE
360 370 380 390 400
QQLAQMAFWP LRLYHSLLGG PGVQGDGVHW YSGLLYRLGR MLLPPDSFHP

LGAPRAES
Length:408
Mass (Da):44,829
Last modified:February 1, 1997 - v1
Checksum:iBA397AE2A9357A24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58511 mRNA. Translation: AAC60010.1.
RefSeqiNP_990288.1. NM_204957.2.
UniGeneiGga.473.

Genome annotation databases

EnsembliENSGALT00000018516; ENSGALP00000018493; ENSGALG00000011347.
GeneIDi395801.
KEGGigga:395801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58511 mRNA. Translation: AAC60010.1.
RefSeqiNP_990288.1. NM_204957.2.
UniGeneiGga.473.

3D structure databases

ProteinModelPortaliQ98938.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018493.

Protein family/group databases

MEROPSiC46.003.

Proteomic databases

PaxDbiQ98938.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000018516; ENSGALP00000018493; ENSGALG00000011347.
GeneIDi395801.
KEGGigga:395801.

Organism-specific databases

CTDi3549.

Phylogenomic databases

eggNOGiKOG3638. Eukaryota.
ENOG410XQA3. LUCA.
GeneTreeiENSGT00390000001117.
HOGENOMiHOG000233428.
HOVERGENiHBG005480.
InParanoidiQ98938.
KOiK11989.
PhylomeDBiQ98938.

Miscellaneous databases

PROiQ98938.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.30.1380.10. 1 hit.
InterProiIPR001657. Hedgehog.
IPR028992. Hedgehog/Intein_dom.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR033385. IHH.
[Graphical view]
PANTHERiPTHR11889:SF39. PTHR11889:SF39. 1 hit.
PfamiPF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view]
PIRSFiPIRSF009400. Peptidase_C46. 1 hit.
PRINTSiPR00632. SONICHHOG.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF55166. SSF55166. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIHH_CHICK
AccessioniPrimary (citable) accession number: Q98938
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.