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Q98938 (IHH_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Indian hedgehog protein

Short name=IHH
Gene names
Name:IHH
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).

Subcellular location

Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side By similarity. Note: The N-terminal peptide remains associated with the cell surface By similarity.

Indian hedgehog protein C-product: Secretedextracellular space By similarity. Note: The C-terminal peptide diffuses from the cell By similarity.

Tissue specificity

Expressed in developing midgut, lung and cartilage of developing long bones in the limb.

Domain

The indian hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain By similarity.

Post-translational modification

The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity By similarity.

Cholesterylation is required for N-product targeting to lipid rafts and multimerization By similarity.

N-palmitoylation is required for N-product multimerization and full activity By similarity.

Sequence similarities

Belongs to the hedgehog family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 408385Indian hedgehog protein
PRO_0000013235
Chain24 – 198175Indian hedgehog protein N-product
PRO_0000013236
Chain199 – 408210Indian hedgehog protein C-product
PRO_0000013237

Sites

Metal binding901Calcium 1 By similarity
Metal binding911Calcium 1 By similarity
Metal binding911Calcium 2 By similarity
Metal binding961Calcium 1 By similarity
Metal binding1261Calcium 1; via carbonyl oxygen By similarity
Metal binding1271Calcium 1 By similarity
Metal binding1271Calcium 2 By similarity
Metal binding1301Calcium 2 By similarity
Metal binding1321Calcium 2 By similarity
Metal binding1411Zinc By similarity
Metal binding1481Zinc By similarity
Metal binding1831Zinc By similarity
Site198 – 1992Cleavage; by autolysis By similarity
Site2441Involved in cholesterol transfer By similarity
Site2681Involved in auto-cleavage By similarity
Site2711Essential for auto-cleavage By similarity

Amino acid modifications

Lipidation241N-palmitoyl cysteine By similarity
Lipidation1981Cholesterol glycine ester By similarity

Sequences

Sequence LengthMass (Da)Tools
Q98938 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: BA397AE2A9357A24

FASTA40844,829
        10         20         30         40         50         60 
MKPARLLLLL SGCALLLAPA VRCCGPGRVV GSRRRPPRKL IPLAYKQFSP NVPEKTLGAS 

        70         80         90        100        110        120 
GRYEGKIARN SERFKELTPN YNPDIIFKDE ENTGADRLMT QRCKDRLNSL AISVMNQWPG 

       130        140        150        160        170        180 
VKLRVTEGWD EDGHHSEESL HYEGRAVDIT TSDRDRNKYG MLARLAVEAG FDWVYYESKA 

       190        200        210        220        230        240 
HIHCSVKSEH SAAAKTGGCF PGRALATLEN GARTPLWALR PGQRVLAMDG AGRPTYSDFL 

       250        260        270        280        290        300 
AFLDKEPRAL TAFHVIETRQ PPRRLALTPT HLLFVADNAS APAAQFRPTF ASHVQPGHFV 

       310        320        330        340        350        360 
LVAVGSGGLQ PAEVVGVRGR TDVGAYAPLT RHGTLVVDDV VASCFALVRE QQLAQMAFWP 

       370        380        390        400 
LRLYHSLLGG PGVQGDGVHW YSGLLYRLGR MLLPPDSFHP LGAPRAES 

« Hide

References

[1]"Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein."
Vortkamp A., Lee K., Lanske B., Segre G.V., Kronenberg H.M., Tabin C.J.
Science 273:613-622(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U58511 mRNA. Translation: AAC60010.1.
RefSeqNP_990288.1. NM_204957.1.
UniGeneGga.473.

3D structure databases

ProteinModelPortalQ98938.
SMRQ98938. Positions 39-195.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000018493.

Protein family/group databases

MEROPSC46.003.

Proteomic databases

PaxDbQ98938.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID395801.
KEGGgga:395801.

Organism-specific databases

CTD3549.

Phylogenomic databases

eggNOGNOG250647.
HOGENOMHOG000233428.
HOVERGENHBG005480.
InParanoidQ98938.
KOK11989.
PhylomeDBQ98938.

Family and domain databases

Gene3D3.30.1380.10. 1 hit.
InterProIPR001657. Hedgehog.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
[Graphical view]
PfamPF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view]
PIRSFPIRSF009400. Peptidase_C46. 1 hit.
PRINTSPR00632. SONICHHOG.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF55166. SSF55166. 1 hit.
ProtoNetSearch...

Other

NextBio20815869.
PROQ98938.

Entry information

Entry nameIHH_CHICK
AccessionPrimary (citable) accession number: Q98938
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families