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Q98931 (LRP8_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Low-density lipoprotein receptor-related protein 8
Short name=LRP-8
Alternative name(s):
Apolipoprotein E receptor 2
Protein LR8B
Gene names
Name:LRP8
Synonyms:LR8B
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length917 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. Also binds alpha2-macroglobulin. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development By similarity.

Subunit structure

Reelin associates with two or more receptor molecules. Interacts with DAB1 and JNK-interacting proteins By similarity. Ref.2

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Mainly in brain.

Post-translational modification

O-glycosylated By similarity.

Tyrosine phosphorylated upon apoE binding By similarity.

Sequence similarities

Belongs to the LDLR family.

Contains 2 EGF-like domains.

Contains 7 LDL-receptor class A domains.

Contains 5 LDL-receptor class B repeats.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainEGF-like domain
Repeat
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendocytosis

Inferred from electronic annotation. Source: UniProtKB-KW

ventral spinal cord development

Inferred from expression pattern PubMed 20711475. Source: UniProtKB

   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced.
Isoform 1 (identifier: Q98931-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 917893Low-density lipoprotein receptor-related protein 8
PRO_0000017334

Regions

Topological domain25 – 838814Extracellular Potential
Transmembrane839 – 86123Helical; Potential
Topological domain862 – 91756Cytoplasmic Potential
Domain28 – 6437LDL-receptor class A 1
Domain67 – 10539LDL-receptor class A 2
Domain108 – 14639LDL-receptor class A 3
Domain148 – 18437LDL-receptor class A 4
Domain187 – 22539LDL-receptor class A 5
Domain272 – 30837LDL-receptor class A 6
Domain312 – 35140LDL-receptor class A 7
Domain346 – 39045EGF-like 1
Domain391 – 43040EGF-like 2; calcium-binding Potential
Repeat476 – 52247LDL-receptor class B 1
Repeat523 – 56543LDL-receptor class B 2
Repeat566 – 60944LDL-receptor class B 3
Repeat610 – 65243LDL-receptor class B 4
Repeat653 – 69543LDL-receptor class B 5
Region754 – 81360Clustered O-linked oligosaccharides

Amino acid modifications

Glycosylation1581N-linked (GlcNAc...) Potential
Glycosylation1961N-linked (GlcNAc...) Potential
Glycosylation5321N-linked (GlcNAc...) Potential
Glycosylation6281N-linked (GlcNAc...) Potential
Glycosylation7821N-linked (GlcNAc...) Potential
Glycosylation8201N-linked (GlcNAc...) Potential
Disulfide bond29 ↔ 41 By similarity
Disulfide bond36 ↔ 54 By similarity
Disulfide bond48 ↔ 63 By similarity
Disulfide bond68 ↔ 80 By similarity
Disulfide bond75 ↔ 93 By similarity
Disulfide bond87 ↔ 104 By similarity
Disulfide bond109 ↔ 123 By similarity
Disulfide bond116 ↔ 136 By similarity
Disulfide bond130 ↔ 145 By similarity
Disulfide bond149 ↔ 161 By similarity
Disulfide bond156 ↔ 174 By similarity
Disulfide bond168 ↔ 183 By similarity
Disulfide bond188 ↔ 200 By similarity
Disulfide bond195 ↔ 213 By similarity
Disulfide bond207 ↔ 224 By similarity
Disulfide bond234 ↔ 246 By similarity
Disulfide bond241 ↔ 259 By similarity
Disulfide bond253 ↔ 268 By similarity
Disulfide bond273 ↔ 285 By similarity
Disulfide bond280 ↔ 298 By similarity
Disulfide bond292 ↔ 307 By similarity
Disulfide bond313 ↔ 326 By similarity
Disulfide bond321 ↔ 339 By similarity
Disulfide bond333 ↔ 350 By similarity
Disulfide bond355 ↔ 366 By similarity
Disulfide bond362 ↔ 375 By similarity
Disulfide bond377 ↔ 389 By similarity
Disulfide bond395 ↔ 405 By similarity
Disulfide bond401 ↔ 414 By similarity
Disulfide bond416 ↔ 429 By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: EF85E4D49441436A

FASTA917101,379
        10         20         30         40         50         60 
MCRPALARLL LLQLLLLKLY LGKGAMKECD KDQFQCRNER CIPAVWACDE DNDCSDNSDE 

        70         80         90        100        110        120 
ADCPKKTCAE TDFACDNGHC IPDRWKCDGE EECSDGSDES EAACTKQVCP AEKISCGDLS 

       130        140        150        160        170        180 
NKCIPSSWRC DGQKDCESGI DEAGCAPACS PDEFQCSNKT CISINFVCDG YNNCGDGSDE 

       190        200        210        220        230        240 
KKCSPLTCSP NEFQCNNSVC IPQLWVCDNQ ADCEDHSDES IERCGYDAKA FNTCAAHEFQ 

       250        260        270        280        290        300 
CGNGECILLN WKCDGDEDCK DKSDEQDCPL VTCRPDEFQC GDGTCIHGAK QCDKVHDCPD 

       310        320        330        340        350        360 
NSDEAGCVQE SACESPSKFQ CKSGECIDGG KVCDLHRDCR DWSDEPLKEC GINECSLNNG 

       370        380        390        400        410        420 
GCSHICKDLK IGYECECPPG YKLLDKKTCG DIDECENPDA CSQICINYKG DYKCECYEGY 

       430        440        450        460        470        480 
EMDTLSKNCK AVGKSPYLIF TNRHEVRKID LVKRDYSRII PMLKNVVALD VEVATNRIYW 

       490        500        510        520        530        540 
CDLFYRKIYS AYIDKASDTA EQVILIDSQL NSPEGVAIDW VHKNIYWTDS GNKTISVATA 

       550        560        570        580        590        600 
DGSRRRTVFN SDLSEPRAIA VDPTRRFMYW SDWGDKAKIE KAGLNGVGRQ VLVSDNIEWP 

       610        620        630        640        650        660 
NGITLDLLNQ RLYWVDSKLH SLSCIDFNGS NREVLISSID DLSHPFGLAV FEDRVFWTDL 

       670        680        690        700        710        720 
ENEAIFSANR LSGLDISVLA ENLNNPHDIV VFHELKQPKA PDSCELSPQP NGGCEYLCLP 

       730        740        750        760        770        780 
APHISPRSPK FTCACPDNMW LGPDMKKCYK ELPTTPATVE VPTTTTSHPA ATSTVTVTGS 

       790        800        810        820        830        840 
ANTTTAVIPR AVSEATTAIP SSHSTTSLLI DSEMTTGNSN LSQHYSNNGQ GFDSTVTAAV 

       850        860        870        880        890        900 
IGIVIPVVVI GLLCMGGYLI WRNWKRKNTK SMNFDNPVYR KTTEEEDEDE IHIGRTAQIG 

       910 
HVYPARVALS LEDDGLP

« Hide

References

[1]"A new LDL receptor homologue with 8 ligand binding repeats in brain of chicken and mouse."
Novak S., Hiesberger T., Schneider W.J., Nimpf J.
J. Biol. Chem. 271:11732-11736(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: White leghorn.
Tissue: Brain.
[2]"Alternative splicing in the ligand binding domain of mouse ApoE receptor-2 produces receptor variants binding reelin but not alpha2-macroglobulin."
Brandes C., Kahr L., Stockinger W., Hiesberger T., Schneider W.J., Nimpf J.
J. Biol. Chem. 276:22160-22169(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, INTERACTION WITH ALPHA2-MACROGLOBULIN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X97001 mRNA. Translation: CAA65729.1.
IPIIPI00581287.
RefSeqNP_990517.1. NM_205186.1.
UniGeneGga.3461.

3D structure databases

ProteinModelPortalQ98931.
SMRQ98931. Positions 270-308, 351-432.
ModBaseSearch...

Proteomic databases

PaxDbQ98931.
PRIDEQ98931.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396102.
KEGGgga:396102.

Organism-specific databases

CTD7804.

Phylogenomic databases

eggNOGNOG255913.
HOGENOMHOG000115656.
HOVERGENHBG006250.
InParanoidQ98931.

Family and domain databases

Gene3D2.120.10.30. 1 hit.
4.10.400.10. 8 hits.
InterProIPR011042. 6-blade_b-propeller_TolB-like.
IPR026823. cEGF.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt.
IPR023415. LDLR_class-A_CS.
IPR000033. LDLR_classB_rpt.
IPR002172. LDrepeatLR_classA_rpt.
[Graphical view]
PfamPF12662. cEGF. 1 hit.
PF00057. Ldl_recept_a. 8 hits.
PF00058. Ldl_recept_b. 5 hits.
[Graphical view]
SMARTSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00192. LDLa. 8 hits.
SM00135. LY. 5 hits.
[Graphical view]
SUPFAMSSF57184. Grow_fac_recept. 1 hit.
SSF57424. LDL_rcpt_classA_cys-rich. 8 hits.
PROSITEPS00010. ASX_HYDROXYL. 2 hits.
PS00022. EGF_1. False negative.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS01209. LDLRA_1. 7 hits.
PS50068. LDLRA_2. 8 hits.
PS51120. LDLRB. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20816160.

Entry information

Entry nameLRP8_CHICK
AccessionPrimary (citable) accession number: Q98931
Secondary accession number(s): Q90883
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: February 1, 1997
Last modified: April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents