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Protein

Low-density lipoprotein receptor-related protein 8

Gene

LRP8

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. Also binds alpha2-macroglobulin. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • ventral spinal cord development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Low-density lipoprotein receptor-related protein 8
Short name:
LRP-8
Alternative name(s):
Apolipoprotein E receptor 2
Protein LR8B
Gene namesi
Name:LRP8
Synonyms:LR8B
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 838814ExtracellularSequence analysisAdd
BLAST
Transmembranei839 – 86123HelicalSequence analysisAdd
BLAST
Topological domaini862 – 91756CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 917893Low-density lipoprotein receptor-related protein 8PRO_0000017334Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 41By similarity
Disulfide bondi36 ↔ 54By similarity
Disulfide bondi48 ↔ 63By similarity
Disulfide bondi68 ↔ 80By similarity
Disulfide bondi75 ↔ 93By similarity
Disulfide bondi87 ↔ 104By similarity
Disulfide bondi109 ↔ 123By similarity
Disulfide bondi116 ↔ 136By similarity
Disulfide bondi130 ↔ 145By similarity
Disulfide bondi149 ↔ 161By similarity
Disulfide bondi156 ↔ 174By similarity
Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence analysis
Disulfide bondi168 ↔ 183By similarity
Disulfide bondi188 ↔ 200By similarity
Disulfide bondi195 ↔ 213By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence analysis
Disulfide bondi207 ↔ 224By similarity
Disulfide bondi234 ↔ 246By similarity
Disulfide bondi241 ↔ 259By similarity
Disulfide bondi253 ↔ 268By similarity
Disulfide bondi273 ↔ 285By similarity
Disulfide bondi280 ↔ 298By similarity
Disulfide bondi292 ↔ 307By similarity
Disulfide bondi313 ↔ 326By similarity
Disulfide bondi321 ↔ 339By similarity
Disulfide bondi333 ↔ 350By similarity
Disulfide bondi355 ↔ 366By similarity
Disulfide bondi362 ↔ 375By similarity
Disulfide bondi377 ↔ 389By similarity
Disulfide bondi395 ↔ 405By similarity
Disulfide bondi401 ↔ 414By similarity
Disulfide bondi416 ↔ 429By similarity
Glycosylationi532 – 5321N-linked (GlcNAc...)Sequence analysis
Glycosylationi628 – 6281N-linked (GlcNAc...)Sequence analysis
Glycosylationi782 – 7821N-linked (GlcNAc...)Sequence analysis
Glycosylationi820 – 8201N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

O-glycosylated.By similarity
Tyrosine phosphorylated upon apoE binding.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ98931.
PRIDEiQ98931.

Expressioni

Tissue specificityi

Mainly in brain.

Interactioni

Subunit structurei

Reelin associates with two or more receptor molecules. Interacts with DAB1 and JNK-interacting proteins (By similarity).By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000017370.

Structurei

3D structure databases

ProteinModelPortaliQ98931.
SMRiQ98931. Positions 270-308, 351-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 6437LDL-receptor class A 1PROSITE-ProRule annotationAdd
BLAST
Domaini67 – 10539LDL-receptor class A 2PROSITE-ProRule annotationAdd
BLAST
Domaini108 – 14639LDL-receptor class A 3PROSITE-ProRule annotationAdd
BLAST
Domaini148 – 18437LDL-receptor class A 4PROSITE-ProRule annotationAdd
BLAST
Domaini187 – 22539LDL-receptor class A 5PROSITE-ProRule annotationAdd
BLAST
Domaini272 – 30837LDL-receptor class A 6PROSITE-ProRule annotationAdd
BLAST
Domaini312 – 35140LDL-receptor class A 7PROSITE-ProRule annotationAdd
BLAST
Domaini346 – 39045EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini391 – 43040EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Repeati476 – 52247LDL-receptor class B 1Add
BLAST
Repeati523 – 56543LDL-receptor class B 2Add
BLAST
Repeati566 – 60944LDL-receptor class B 3Add
BLAST
Repeati610 – 65243LDL-receptor class B 4Add
BLAST
Repeati653 – 69543LDL-receptor class B 5Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni754 – 81360Clustered O-linked oligosaccharidesAdd
BLAST

Sequence similaritiesi

Belongs to the LDLR family.Curated
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 7 LDL-receptor class A domains.PROSITE-ProRule annotation
Contains 5 LDL-receptor class B repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPT5. Eukaryota.
ENOG410YQ6J. LUCA.
HOGENOMiHOG000115656.
HOVERGENiHBG006250.
InParanoidiQ98931.
PhylomeDBiQ98931.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
4.10.400.10. 8 hits.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
IPR023415. LDLR_class-A_CS.
IPR000033. LDLR_classB_rpt.
IPR002172. LDrepeatLR_classA_rpt.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 8 hits.
PF00058. Ldl_recept_b. 5 hits.
[Graphical view]
PRINTSiPR00261. LDLRECEPTOR.
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00192. LDLa. 8 hits.
SM00135. LY. 5 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 2 hits.
SSF57424. SSF57424. 7 hits.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS01209. LDLRA_1. 7 hits.
PS50068. LDLRA_2. 8 hits.
PS51120. LDLRB. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.

Isoform 1 (identifier: Q98931-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCRPALARLL LLQLLLLKLY LGKGAMKECD KDQFQCRNER CIPAVWACDE
60 70 80 90 100
DNDCSDNSDE ADCPKKTCAE TDFACDNGHC IPDRWKCDGE EECSDGSDES
110 120 130 140 150
EAACTKQVCP AEKISCGDLS NKCIPSSWRC DGQKDCESGI DEAGCAPACS
160 170 180 190 200
PDEFQCSNKT CISINFVCDG YNNCGDGSDE KKCSPLTCSP NEFQCNNSVC
210 220 230 240 250
IPQLWVCDNQ ADCEDHSDES IERCGYDAKA FNTCAAHEFQ CGNGECILLN
260 270 280 290 300
WKCDGDEDCK DKSDEQDCPL VTCRPDEFQC GDGTCIHGAK QCDKVHDCPD
310 320 330 340 350
NSDEAGCVQE SACESPSKFQ CKSGECIDGG KVCDLHRDCR DWSDEPLKEC
360 370 380 390 400
GINECSLNNG GCSHICKDLK IGYECECPPG YKLLDKKTCG DIDECENPDA
410 420 430 440 450
CSQICINYKG DYKCECYEGY EMDTLSKNCK AVGKSPYLIF TNRHEVRKID
460 470 480 490 500
LVKRDYSRII PMLKNVVALD VEVATNRIYW CDLFYRKIYS AYIDKASDTA
510 520 530 540 550
EQVILIDSQL NSPEGVAIDW VHKNIYWTDS GNKTISVATA DGSRRRTVFN
560 570 580 590 600
SDLSEPRAIA VDPTRRFMYW SDWGDKAKIE KAGLNGVGRQ VLVSDNIEWP
610 620 630 640 650
NGITLDLLNQ RLYWVDSKLH SLSCIDFNGS NREVLISSID DLSHPFGLAV
660 670 680 690 700
FEDRVFWTDL ENEAIFSANR LSGLDISVLA ENLNNPHDIV VFHELKQPKA
710 720 730 740 750
PDSCELSPQP NGGCEYLCLP APHISPRSPK FTCACPDNMW LGPDMKKCYK
760 770 780 790 800
ELPTTPATVE VPTTTTSHPA ATSTVTVTGS ANTTTAVIPR AVSEATTAIP
810 820 830 840 850
SSHSTTSLLI DSEMTTGNSN LSQHYSNNGQ GFDSTVTAAV IGIVIPVVVI
860 870 880 890 900
GLLCMGGYLI WRNWKRKNTK SMNFDNPVYR KTTEEEDEDE IHIGRTAQIG
910
HVYPARVALS LEDDGLP
Length:917
Mass (Da):101,379
Last modified:February 1, 1997 - v1
Checksum:iEF85E4D49441436A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97001 mRNA. Translation: CAA65729.1.
RefSeqiNP_990517.1. NM_205186.1. [Q98931-1]
UniGeneiGga.3461.

Genome annotation databases

GeneIDi396102.
KEGGigga:396102.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97001 mRNA. Translation: CAA65729.1.
RefSeqiNP_990517.1. NM_205186.1. [Q98931-1]
UniGeneiGga.3461.

3D structure databases

ProteinModelPortaliQ98931.
SMRiQ98931. Positions 270-308, 351-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000017370.

Proteomic databases

PaxDbiQ98931.
PRIDEiQ98931.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396102.
KEGGigga:396102.

Organism-specific databases

CTDi7804.

Phylogenomic databases

eggNOGiENOG410IPT5. Eukaryota.
ENOG410YQ6J. LUCA.
HOGENOMiHOG000115656.
HOVERGENiHBG006250.
InParanoidiQ98931.
PhylomeDBiQ98931.

Miscellaneous databases

NextBioi20816160.
PROiQ98931.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
4.10.400.10. 8 hits.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
IPR023415. LDLR_class-A_CS.
IPR000033. LDLR_classB_rpt.
IPR002172. LDrepeatLR_classA_rpt.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 8 hits.
PF00058. Ldl_recept_b. 5 hits.
[Graphical view]
PRINTSiPR00261. LDLRECEPTOR.
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00192. LDLa. 8 hits.
SM00135. LY. 5 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 2 hits.
SSF57424. SSF57424. 7 hits.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS01209. LDLRA_1. 7 hits.
PS50068. LDLRA_2. 8 hits.
PS51120. LDLRB. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A new LDL receptor homologue with 8 ligand binding repeats in brain of chicken and mouse."
    Novak S., Hiesberger T., Schneider W.J., Nimpf J.
    J. Biol. Chem. 271:11732-11736(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: White leghorn.
    Tissue: Brain.
  2. "Alternative splicing in the ligand binding domain of mouse ApoE receptor-2 produces receptor variants binding reelin but not alpha2-macroglobulin."
    Brandes C., Kahr L., Stockinger W., Hiesberger T., Schneider W.J., Nimpf J.
    J. Biol. Chem. 276:22160-22169(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, INTERACTION WITH ALPHA2-MACROGLOBULIN.

Entry informationi

Entry nameiLRP8_CHICK
AccessioniPrimary (citable) accession number: Q98931
Secondary accession number(s): Q90883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: February 1, 1997
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.