Q988H0 (NADE_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	mll6744

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152188
Regions
	Nucleotide binding	33 – 40	8	ATP By similarity
Sites
	Active site	35	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q988H0 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 553D2F853DA2966C	FASTA	319	35,517
	10 20 30 40 50 60 MLALDARAEV DRIVDTLRKQ VLGTLRRRGV VVGLSGGIDS SVVATLCTRA FGKDKVLGLF 70 80 90 100 110 120 MPERDSSGDS LRLGRRVAAQ LGIENILEDI GPAVEAVGAY RRQLEAIRTV VPDYGDGWKC 130 140 150 160 170 180 KLVIEPVLES NGLNITRLTV QDPEGKVDTV RLSPAAYLQI VAATNYKQRL RKMTEYYHAD 190 200 210 220 230 240 RLKYAVAGTP NRLEYDQGFF VKQGDGTADV MPIVHLYKTQ VYQLAEYLGV DEEIRQRPPT 250 260 270 280 290 300 TDTFSMAQSQ EEFYFALPYH LMDLCLYGLN HGIASDEVAA VAGLTEPQLQ KVYKDIDAKR 310 RAAHYLHARP LLSVEIGED

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB52980.1.
RefSeq	NP_107194.1. NC_002678.2.
3D structure databases
ProteinModelPortal	Q988H0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1229849.
GenomeReviews	Gene locus mll6744 in contig BA000012_GR.
KEGG	mlo:mll6744.
NMPDR	fig|266835.1.peg.5295.
PATRIC	22484969. VBIMesLot2464_5364.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	AHLYKSQ.
ProtClustDB	PRK00876.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_RHILO

Accession

Primary (citable) accession number: Q988H0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program