Q98862 (IHH_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Indian hedgehog B protein
Short name=IHHB

Alternative name(s):
Echidna hedgehog protein
Short name=EHH

Cleaved into the following 2 chains:

1. Indian hedgehog B protein N-product
2. Indian hedgehog B protein C-product

Gene names

Name:	ihhb
Synonyms:	ehh, ihh

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Intercellular signal essential for a variety of patterning events during development. Signal produced by the notochord that induces somite patterning and muscle pioneer differentiation.
Subcellular location	Indian hedgehog B protein N-product: Cell membrane; Lipid-anchor; Extracellular side By similarity. Note: The N-terminal peptide remains associated with the cell surface By similarity. Indian hedgehog B protein C-product: Secreted › extracellular space By similarity. Note: The C-terminal peptide diffuses from the cell By similarity.
Tissue specificity	Expressed exclusively in the notochord.
Developmental stage	First detectable at the mid-gastrula stage. Disappears at the end of the somitogenesis.
Post-translational modification	The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity By similarity. Cholesterylation is required for N-product targeting to lipid rafts and multimerization By similarity. N-palmitoylation is required for N-product multimerization and full activity By similarity.
Sequence similarities	Belongs to the hedgehog family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	Signal
Molecular function	Developmental protein Hydrolase Protease
PTM	Autocatalytic cleavage Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell-cell signaling Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW striated muscle cell development Inferred from genetic interaction. Source: ZFIN
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 412	389	Indian hedgehog B protein		PRO_0000013241
	Chain	24 – 197	174	Indian hedgehog B protein N-product		PRO_0000013242
	Chain	198 – 412	215	Indian hedgehog B protein C-product		PRO_0000013243
Sites
	Site	197 – 198	2	Cleavage; by autolysis By similarity
	Site	246	1	Involved in cholesterol transfer By similarity
	Site	269	1	Involved in auto-cleavage By similarity
	Site	272	1	Essential for auto-cleavage By similarity
Amino acid modifications
	Lipidation	24	1	N-palmitoyl cysteine By similarity
	Lipidation	197	1	Cholesterol glycine ester By similarity
Experimental info
	Sequence conflict	121	1	R → K Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q98862 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 21D43F052A278CA1	FASTA	412	45,746
	10 20 30 40 50 60 MRLSTAAALL TGFILAFSPA YDGCGPGRGY GKRRTPRKLT PLAYKQFSPN VAEKTLGASG 70 80 90 100 110 120 RYEGKVTPSS ERFKELTPNY NPDIIFKDEE NTGADRMMTQ RCKDKLNSLA ISVMNLWPGV 130 140 150 160 170 180 RLRVTEGWDE DGLHSEESLH YEGRAVDITT SDRDRNKYRM LARLAVEAGF DWVYYESKGH 190 200 210 220 230 240 VHCSVKSEHS VAAKTGGCFP GRALVTMKDG SHRQIRDLQA GDLVLASEGS DGTGDLIYSE 250 260 270 280 290 300 VLTFLDRRPI TQKHFYVIRT EDGASVSLTA AHLLFMRVGN CSNRGEPKPG AVRTIFASDA 310 320 330 340 350 360 QVGQCLLLGK LRKRFSQITH VGVREDQGLY PPLTAHGTVV VNDVLTSCYA AVNRQRLAHW 370 380 390 400 410 AFAPLRLLYS WTGPDQVLKN GLHWYSQVLI GLGKLLLDSE LFHPLALEAT ER

References

[1]	"Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish." Currie P.D., Ingham P.W. Nature 382:452-455(1996) [PubMed: 8684485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Evolutionary analyses of hedgehog and Hoxd-10 genes in fish species closely related to the zebrafish." Zardoya R., Abouheif E., Meyer A. Proc. Natl. Acad. Sci. U.S.A. 93:13036-13041(1996) [PubMed: 8917540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-170. Tissue: Muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08426 mRNA. Translation: CAA69702.1.
IPI	IPI00511914.
RefSeq	NP_571163.1. NM_131088.1.
UniGene	Dr.75090.
3D structure databases
ProteinModelPortal	Q98862.
SMR	Q98862. Positions 38-194.
ModBase	Search...
Protein-protein interaction databases
STRING	Q98862.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	30299.
KEGG	dre:30299.
Organism-specific databases
CTD	30299.
ZFIN	ZDB-GENE-980526-135. ihhb.
Phylogenomic databases
GeneTree	ENSGT00390000001117.
HOGENOM	HBG403179.
HOVERGEN	HBG005480.
InParanoid	Q98862.
OrthoDB	EOG4F1X39.
Gene expression databases
ArrayExpress	Q98862.
Bgee	Q98862.
Family and domain databases
InterPro	IPR009045. Hedgehog/DD-peptidase. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR000320. Hedgehog_signaling_dom. IPR006141. Intein_splice_site. IPR001657. Peptidase_C46. IPR001767. Peptidase_C46_hint. [Graphical view]
Gene3D	G3DSA:3.30.1380.10. Hedgehog/DD-pept_Zn-bd. 1 hit.
KO	K06224.
Pfam	PF01085. HH_signal. 1 hit. PF01079. Hint. 1 hit. [Graphical view]
PIRSF	PIRSF009400. Peptidase_C46. 1 hit.
PRINTS	PR00632. SONICHHOG.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF55166. Hedgehog_sig_N. 1 hit.
PROSITE	PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IHH_DANRE

Accession

Primary (citable) accession number: Q98862

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 1, 1997
Last modified:	November 16, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program