Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q98862 (IHH_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Indian hedgehog B protein

Short name=IHHB
Alternative name(s):
Echidna hedgehog protein
Short name=EHH
Gene names
Name:ihhb
Synonyms:ehh, ihh
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Intercellular signal essential for a variety of patterning events during development. Signal produced by the notochord that induces somite patterning and muscle pioneer differentiation.

Subcellular location

Indian hedgehog B protein N-product: Cell membrane; Lipid-anchor; Extracellular side By similarity. Note: The N-terminal peptide remains associated with the cell surface By similarity.

Indian hedgehog B protein C-product: Secretedextracellular space By similarity. Note: The C-terminal peptide diffuses from the cell By similarity.

Tissue specificity

Expressed exclusively in the notochord.

Developmental stage

First detectable at the mid-gastrula stage. Disappears at the end of the somitogenesis.

Domain

The indian hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain By similarity.

Post-translational modification

The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity By similarity.

Cholesterylation is required for N-product targeting to lipid rafts and multimerization By similarity.

N-palmitoylation is required for N-product multimerization and full activity By similarity.

Sequence similarities

Belongs to the hedgehog family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 412389Indian hedgehog B protein
PRO_0000013241
Chain24 – 197174Indian hedgehog B protein N-product
PRO_0000013242
Chain198 – 412215Indian hedgehog B protein C-product
PRO_0000013243

Sites

Metal binding891Calcium 1 By similarity
Metal binding901Calcium 1 By similarity
Metal binding901Calcium 2 By similarity
Metal binding951Calcium 1 By similarity
Metal binding1251Calcium 1; via carbonyl oxygen By similarity
Metal binding1261Calcium 1 By similarity
Metal binding1261Calcium 2 By similarity
Metal binding1291Calcium 2 By similarity
Metal binding1311Calcium 2 By similarity
Metal binding1401Zinc By similarity
Metal binding1471Zinc By similarity
Metal binding1821Zinc By similarity
Site197 – 1982Cleavage; by autolysis By similarity
Site2461Involved in cholesterol transfer By similarity
Site2691Involved in auto-cleavage By similarity
Site2721Essential for auto-cleavage By similarity

Amino acid modifications

Lipidation241N-palmitoyl cysteine By similarity
Lipidation1971Cholesterol glycine ester By similarity

Experimental info

Sequence conflict1211R → K Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q98862 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 21D43F052A278CA1

FASTA41245,746
        10         20         30         40         50         60 
MRLSTAAALL TGFILAFSPA YDGCGPGRGY GKRRTPRKLT PLAYKQFSPN VAEKTLGASG 

        70         80         90        100        110        120 
RYEGKVTPSS ERFKELTPNY NPDIIFKDEE NTGADRMMTQ RCKDKLNSLA ISVMNLWPGV 

       130        140        150        160        170        180 
RLRVTEGWDE DGLHSEESLH YEGRAVDITT SDRDRNKYRM LARLAVEAGF DWVYYESKGH 

       190        200        210        220        230        240 
VHCSVKSEHS VAAKTGGCFP GRALVTMKDG SHRQIRDLQA GDLVLASEGS DGTGDLIYSE 

       250        260        270        280        290        300 
VLTFLDRRPI TQKHFYVIRT EDGASVSLTA AHLLFMRVGN CSNRGEPKPG AVRTIFASDA 

       310        320        330        340        350        360 
QVGQCLLLGK LRKRFSQITH VGVREDQGLY PPLTAHGTVV VNDVLTSCYA AVNRQRLAHW 

       370        380        390        400        410 
AFAPLRLLYS WTGPDQVLKN GLHWYSQVLI GLGKLLLDSE LFHPLALEAT ER 

« Hide

References

[1]"Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish."
Currie P.D., Ingham P.W.
Nature 382:452-455(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Evolutionary analyses of hedgehog and Hoxd-10 genes in fish species closely related to the zebrafish."
Zardoya R., Abouheif E., Meyer A.
Proc. Natl. Acad. Sci. U.S.A. 93:13036-13041(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-170.
Tissue: Muscle.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y08426 mRNA. Translation: CAA69702.1.
RefSeqNP_571163.1. NM_131088.1.
UniGeneDr.75090.

3D structure databases

ProteinModelPortalQ98862.
SMRQ98862. Positions 38-194.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID30299.
KEGGdre:30299.

Organism-specific databases

CTD30299.
ZFINZDB-GENE-980526-135. ihhb.

Phylogenomic databases

eggNOGNOG250647.
HOGENOMHOG000233428.
HOVERGENHBG005480.
InParanoidQ98862.
KOK11989.
PhylomeDBQ98862.

Family and domain databases

Gene3D3.30.1380.10. 1 hit.
InterProIPR001657. Hedgehog.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
[Graphical view]
PfamPF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view]
PIRSFPIRSF009400. Peptidase_C46. 1 hit.
PRINTSPR00632. SONICHHOG.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF55166. SSF55166. 1 hit.
PROSITEPS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806741.

Entry information

Entry nameIHH_DANRE
AccessionPrimary (citable) accession number: Q98862
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families