Reviewed, UniProtKB/Swiss-Prot Q987N5 (PANE_RHILO)

Names and origin

Protein names

Recommended name:
    Putative 2-dehydropantoate 2-reductase
    EC=1.1.1.169
Alternative name(s):
    Ketopantoate reductase
      Short name=KPA reductase
      Short name=KPR

Gene names

Ordered Locus Names:

mll6982

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid By similarity.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP or NADPH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Putative 2-dehydropantoate 2-reductase		PRO_0000157317
Regions
	Nucleotide binding	7 – 12	6	NADP By similarity
Sites
	Active site	205	1	Proton donor By similarity
	Binding site	103	1	NADP; via amide nitrogen By similarity
	Binding site	103	1	Substrate By similarity
	Binding site	209	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	274	1	Substrate By similarity
	Binding site	286	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q987N5-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: E1C28123762ACFD2	FASTA	326	34,712
	10 20 30 40 50 60 MKITIFGAGA IGGYLAAKLA IAGRTDLSIV ARGAHLEAIQ ANGLRLIEDG EESMAPVRAA 70 80 90 100 110 120 AKAEELGAQD YVVLALKAHS LTPALDQIAP LLGDHTSVVT MQNGVPWWYF HGVGGPLEGT 130 140 150 160 170 180 RLNAVDPGGA IWQRIGPQRV IGSVVYPAVE VDAPGLIRHV EGKRFSLGEP SGERSERVTL 190 200 210 220 230 240 LAEEMVKAGL QAPVRDDIRS EIWVKLWGNL SFNPISALTG STLAAIVADE GTRALARTMM 250 260 270 280 290 300 LEAQAIGESL GVRFPIGVDR RIKGAGDVGE HKTSMLQDLE RGRPMEIDAL VSAVQELGRL 310 320 VDKPTPTIDA VLALVRRLAV ERGCYS

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB53168.1.
RefSeq	NP_107382.1.
3D structure databases
SMR	Q987N5. Positions 1-315.
ModBase	Search...
Genome annotation databases
GeneID	1230037.
GenomeReviews	Gene locus mll6982 in contig BA000012_GR.
KEGG	mlo:mll6982.
NMPDR	fig|266835.1.peg.5483.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG668370.
OMA	VVQEMGR.
Enzyme and pathway databases
BRENDA	1.1.1.169. 3315.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21708:SF21. ApbA. 1 hit.
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_RHILO

Accession

Primary (citable) accession number: Q987N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)