Q986N6 (TPIS2_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase 2
Short name=TIM 2
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase 2

Gene names

Name:	tpiA2
Ordered Locus Names:	mlr7275

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Triosephosphate isomerase 2 HAMAP MF_00147_B		PRO_0000090275
Sites
	Active site	97	1	Electrophile By similarity
	Active site	170	1	Proton acceptor By similarity
	Binding site	12	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q986N6 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 336F73D7EB87F653	FASTA	254	27,199
	10 20 30 40 50 60 MRVVYWVGTS WKMNKTLAEA LDFAERLAGF IPGFDDRIQP FVIPPFTAVR EVKRALSSTR 70 80 90 100 110 120 IKVGAQNMHW ADAGAWTGEI SPPMLRDCGL DLVELGHSER REHFGETDRT VGLKTAAAVK 130 140 150 160 170 180 HGMIPLICVG ETLAERESGQ ADAVLSAQVE GALQFLEGEA KTAKILFAYE PVWAIGDKGI 190 200 210 220 230 240 PASADYADKQ QALIKTVAGA LLPSVPPVLY GGSVNPGNAA ELVGQPNIDG LFIGRSAWQA 250 DGYIDILGRA SAAI

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB53417.1.
RefSeq	NP_107631.1. NC_002678.2.
3D structure databases
ProteinModelPortal	Q986N6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1230286.
GenomeReviews	Gene locus mlr7275 in contig BA000012_GR.
KEGG	mlo:mlr7275.
NMPDR	fig|266835.1.peg.5732.
PATRIC	22485865. VBIMesLot2464_5812.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG708281.
OMA	WAIGVNG.
ProtClustDB	PRK00042.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS2_RHILO

Accession

Primary (citable) accession number: Q986N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program