Q986N2 (RISB1_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase 1
Short name=DMRL synthase 1
Short name=Lumazine synthase 1
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase 1 beta chain

Gene names

Name:	ribH1
Synonyms:	ribH
Ordered Locus Names:	mll7281

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 157

157

6,7-dimethyl-8-ribityllumazine synthase 1 HAMAP MF_00178

PRO_0000134792

Secondary structure

1

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157

Helix Strand Turn

Details...

Beta strand

12 – 19

8

Helix

23 – 41

19

Beta strand

44 – 54

11

Helix

55 – 57

3

Helix

58 – 68

11

Beta strand

72 – 79

8

Beta strand

84 – 86

3

Helix

89 – 106

18

Beta strand

110 – 115

6

Helix

124 – 154

31

Sequences

	Sequence		Length	Mass (Da)
	Q986N2 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 26277728CDB09D7B	FASTA	157	17,281
	10 20 30 40 50 60 MNQHSHKDYE TVRIAVVRAR WHADIVDQCV SAFEAEMADI GGDRFAVDVF DVPGAYEIPL 70 80 90 100 110 120 HARTLAETGR YGAVLGTAFV VNGGIYRHEF VASAVIDGMM NVQLSTGVPV LSAVLTPHNY 130 140 150 HDSAEHHRFF FEHFTVKGKE AARACVEILA AREKIAA

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000012 Genomic DNA. Translation: BAB53421.1.

RefSeq

NP_107635.1. NC_002678.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OBX	X-ray	2.53	A/B/C/D/E/F/G/H/I/J	1-157	[»]

ProteinModelPortal

Q986N2.

SMR

Q986N2. Positions 6-155.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1230290.

GenomeReviews

Gene locus mll7281 in contig BA000012_GR.

KEGG

mlo:mll7281.

NMPDR

fig|266835.1.peg.5736.

PATRIC

22485873. VBIMesLot2464_5816.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG311126.

OMA

RRYTAIV.

ProtClustDB

PRK12419.

Family and domain databases

HAMAP

MF_00178. Lumazine_synth.
[Tree]

InterPro

IPR002180. DMRL_synthase.
[Graphical view]

Gene3D

G3DSA:3.40.50.960. DMRL_synthase. 1 hit.

KO

K00794.

PANTHER

PTHR21058. DMRL_synthase. 1 hit.

Pfam

PF00885. DMRL_synthase. 1 hit.
[Graphical view]

SUPFAM

SSF52121. DMRL_synthase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RISB1_RHILO

Accession

Primary (citable) accession number: Q986N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program