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Q986A5 (PUR2_RHILO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:mlr7447
OrganismRhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]
Taxonomic identifier266835 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 425425Phosphoribosylamine--glycine ligase HAMAP MF_00138
PRO_0000151473

Regions

Domain107 – 312206ATP-grasp
Nucleotide binding133 – 19361ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q986A5 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 3ACCB64A47DD7ED7

FASTA42544,487
        10         20         30         40         50         60 
MNVLLLGSGG REHALAWKIA ASPLLTKLYA APGNPGIGAE AELVKLDITD HAAVTAFCQE 

        70         80         90        100        110        120 
KKIDLVVVGP EGPLVAGIAD DLRAENIRVF GPSKAAARLE GSKGFTKDLC ARYNIPTAAY 

       130        140        150        160        170        180 
GRFNDLASAK AYVDQTGAPI VIKADGLAAG KGVTVAMTSD EARAALDACF EGSFGAAGAE 

       190        200        210        220        230        240 
VVVEEFMTGE EASFFCLCDG TTALPFGTAQ DHKRVGDGDV GPNTGGMGAY SPAPVMTPEM 

       250        260        270        280        290        300 
IERTMREIIE PTMRGMAELG APFAGILFAG LMITDKGPKL IEYNTRFGDP ECQVLMMRLK 

       310        320        330        340        350        360 
DDLLVLLNAA VDGQLAHTSI RWRDEAALTV VMAARGYPGT PEKGSVIRGV EQAAGEGVQI 

       370        380        390        400        410        420 
FHAGTAINGG ALVANGGRVL NVTASGATVG EAQKRAYAAL DRIDWPDGFC RRDIGWQAVA 


RERAS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000012 Genomic DNA. Translation: BAB53548.1.
RefSeqNP_107762.1. NC_002678.2.

3D structure databases

ProteinModelPortalQ986A5.
SMRQ986A5. Positions 1-421.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1230417.
GenomeReviewsGene locus mlr7447 in contig BA000012_GR.
KEGGmlo:mlr7447.
NMPDRfig|266835.1.peg.5863.
PATRIC22486137. VBIMesLot2464_5948.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG404060.
OMAMGAYTPL.
ProtClustDBPRK00885.

Family and domain databases

HAMAPMF_00138. GARS.
[Tree]
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.90.600.10. Gars. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01945.
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMsTIGR00877. PurD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_RHILO
AccessionPrimary (citable) accession number: Q986A5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2001
Last modified: January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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