Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q985P4 (GLGB_RHILO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:mlr7587
OrganismRhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]
Taxonomic identifier266835 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium

Protein attributes

Sequence length737 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7377371,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188734

Sites

Active site4191Nucleophile By similarity
Active site4721Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q985P4 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: BF88CA1D72A8AAEB

FASTA73782,526
        10         20         30         40         50         60 
MRKPRVTAAT SGPDGLAPAS DVAAIVAGTH GDPFAVLGVH EVGKGLFARC FVPHAETVTA 

        70         80         90        100        110        120 
YTLTGIEAGA LSRRDDAGFF EGKLSIKKRQ PLRYHAHNAG GDWWLTDPYS FGPVLGPMDD 

       130        140        150        160        170        180 
YYIAEGSHLR LFDKLGAHVI EHEGATGVHF AVWAPNAKRV SVVGDFNDWD GRRHTMRDRR 

       190        200        210        220        230        240 
DTGIWEVFIP DIGAGRPYKY EIIGPDGVRL PLKADPFAFK SELRPATASV VAVPPAHDWG 

       250        260        270        280        290        300 
DEAHRNYWRN ADPRREAVSI YEVHAGSWQL HDDGTFLSWD ELADRLIPYV VETGFTHIEF 

       310        320        330        340        350        360 
MPISEHPYDP SWGYQTTGLY APSARFGDPD GFARFVDGAH RAGVGVILDW VPAHFPVDAH 

       370        380        390        400        410        420 
GLAHFDGTAL YEHADPRKGF HPDWNTAIYN FGRREVVSFL VNNALFWAEK YHVDGLRVDA 

       430        440        450        460        470        480 
VASMLYLDYS RKAGEWIPNE KGGRENLEAV SFLQKMNKEV YGHHPGVMTI AEESTSWPKV 

       490        500        510        520        530        540 
SAPVHEGGLG FGFKWNMGFM HDTLEYFSKE PIFRKHHHND LTFGLTYAFS ENFVLPLSHD 

       550        560        570        580        590        600 
EVVHGKGTLL SKMAGDDWQK FATLRAYYGF MWGYPGKKLL FMGQEFAQRR EWSEARALDW 

       610        620        630        640        650        660 
NLLDFRPHRG VWQTVRDLNY LYRSRPALHG RDCEPEGFSW LIVDDSQNSV FAWVRNAPGG 

       670        680        690        700        710        720 
SPVAVISNFT PVPRDNYRVP LPKAGKWREI INTDASEYGG SGMGNGGMVE ARAEGKNISA 

       730 
TMLLPPLSTI MLELVAD 

« Hide

References

[1]"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti."
Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. expand/collapse author list , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S.
DNA Res. 7:331-338(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MAFF303099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000012 Genomic DNA. Translation: BAB54018.1.
RefSeqNP_107873.1. NC_002678.2.

3D structure databases

ProteinModelPortalQ985P4.
SMRQ985P4. Positions 127-736.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266835.mlr7587.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB54018; BAB54018; BAB54018.
GeneID1230528.
KEGGmlo:mlr7587.
PATRIC22486379. VBIMesLot2464_6068.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAWIERINT.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycMLOT266835:GJ9L-6025-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_RHILO
AccessionPrimary (citable) accession number: Q985P4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: October 1, 2001
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries