Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q985M6 (BETB_RHILO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenase

Short name=BADH
EC=1.2.1.8
Gene names
Name:betB
Ordered Locus Names:mll7608
OrganismRhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]
Taxonomic identifier266835 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid By similarity. HAMAP-Rule MF_00804

Catalytic activity

Betaine aldehyde + NAD+ + H2O = betaine + NADH. HAMAP-Rule MF_00804

Cofactor

Binds 2 potassium ions per subunit By similarity. HAMAP-Rule MF_00804

Pathway

Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1. HAMAP-Rule MF_00804

Subunit structure

Dimer of dimers By similarity. HAMAP-Rule MF_00804

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
   LigandMetal-binding
NAD
NADP
Potassium
   Molecular functionOxidoreductase
   PTMOxidation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycine betaine biosynthetic process from choline

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionbetaine-aldehyde dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487NAD/NADP-dependent betaine aldehyde dehydrogenase HAMAP-Rule MF_00804
PRO_0000056552

Regions

Nucleotide binding149 – 1524NAD/NADP By similarity
Nucleotide binding175 – 1784NAD/NADP By similarity
Nucleotide binding227 – 2326NAD/NADP By similarity

Sites

Active site1611Charge relay system By similarity
Active site2491Proton acceptor By similarity
Active site4611Charge relay system By similarity
Metal binding271Potassium 1; via carbonyl oxygen By similarity
Metal binding931Potassium 1 By similarity
Metal binding4541Potassium 2; via carbonyl oxygen By similarity
Metal binding4571Potassium 2; via carbonyl oxygen By similarity
Binding site2081NAD/NADP; via amide nitrogen By similarity
Binding site2831NAD/NADP By similarity
Binding site3841NAD/NADP By similarity

Amino acid modifications

Modified residue2831Cysteine sulfenic acid (-SOH) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q985M6 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 74CE4AC1F6AC0F5E

FASTA48751,678
        10         20         30         40         50         60 
MRAQPTASHY VNGRYIDDEQ GAPLPVIYPA TGETIAMLRS ATPNVLELAI EAARAAQPAW 

        70         80         90        100        110        120 
ARLKPVERGR ILRRAADILR ARNADLARIE TLDTGKAIQE TLVADAPSAA DCLEYFGGAV 

       130        140        150        160        170        180 
AAYNGEAVDL GGPFAYTRRE ALGVCVGIGA WNYPIQIAGW KSAPALAMGN AMVFKPSENT 

       190        200        210        220        230        240 
PLSALALAEI YSEAGLPDGL FNVVQGYGDV GAGLVGHDVV AKVSVTGSVP TGRKVLSLAG 

       250        260        270        280        290        300 
SKMKHATMEL GGKSPLIVFD DADIENAIGG AMLGNFYSTG QICSNGTRVF VQSGIHDRFV 

       310        320        330        340        350        360 
ERLVERTKKI RIGNPLDPET QMGPLVSKAQ HEKVVGYIGI GKQDGAVLAC GGNVPSLQGF 

       370        380        390        400        410        420 
DGGFFVEPTV FTGVTDTMRI AREEIFGPVM SVLKFDGEDE VIDRANDTEF GLAAGVFTRD 

       430        440        450        460        470        480 
LPRAHRVIAE LQAGTCWINA YNLTPVEIPF GGFKQSGIGR ENSLAALALY SQLKSIYVET 


GDVASPY 

« Hide

References

[1]"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti."
Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. expand/collapse author list , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S.
DNA Res. 7:331-338(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MAFF303099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000012 Genomic DNA. Translation: BAB54036.1.
RefSeqNP_107891.1. NC_002678.2.

3D structure databases

ProteinModelPortalQ985M6.
SMRQ985M6. Positions 7-487.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266835.mll7608.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB54036; BAB54036; BAB54036.
GeneID1230546.
KEGGmlo:mll7608.
PATRIC22486419. VBIMesLot2464_6087.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1012.
HOGENOMHOG000271505.
KOK00130.
OMAAHRTIHR.
OrthoDBEOG6BS8QW.
ProtClustDBPRK13252.

Enzyme and pathway databases

BioCycMLOT266835:GJ9L-6044-MONOMER.
UniPathwayUPA00529; UER00386.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPMF_00804. BADH.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR01804. BADH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBETB_RHILO
AccessionPrimary (citable) accession number: Q985M6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 1, 2001
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways