Reviewed, UniProtKB/Swiss-Prot Q985M5 (BETA_RHILO)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	mll7609

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Choline dehydrogenase HAMAP MF_00750		PRO_0000205593
Regions
	Nucleotide binding	5 – 34	30	FAD Probable
Sites
	Active site	466	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q985M5-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: B3FC4F94EA6B5E21	FASTA	550	60,125
	10 20 30 40 50 60 MLEADFVIIG SGSAGSAMAY RLSEDGKHSV IVIEFGGSDI GPLIQMPSAL SIPLNMSLYD 70 80 90 100 110 120 WGFASEPEPH LGGRVLATPR GKVIGGSSSI NGMVYVRGHA RDFDHWAEEG ATGWGFADVL 130 140 150 160 170 180 PYFKRMEDND GGEDGWRGHG GPLHVQRGSR KNPLYGAFVE AGRQAGFELT DDYNGSKQEG 190 200 210 220 230 240 FGPMEQTISG GRRWSAASAY LKPALKRKNV SLVKGFARRV IIENQRAIGV EIEAHKQIQV 250 260 270 280 290 300 VKARREVIVA ASSINSPKIL MLSGIGPAEH LRENGIAVVA DRPGVGRNLQ DHMELYIQQE 310 320 330 340 350 360 STKPITLNSV LNPFSKALIG AQWLFFKSGL GATNHFEAAA FVRSRAGVDY PDIQYHFIPA 370 380 390 400 410 420 AVRYDGKAAA KSHGFQAHVG PMRSKSRGSV TLRSPDPKAK PVIRFNYMSH PDDWTEFRHC 430 440 450 460 470 480 IRLTREIFGQ SAFDAFRGQE ISPGSHVQSD DDLDVFIRDH AESAYHPCGT CKMGRADDVT 490 500 510 520 530 540 SVVDPECRVI GVDGLRVADS SIFPRVTNGN LNAPSIMTGE KASDHILGRT PLAPSNQEPW 550 INPRWQVSDR

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000012 Genomic DNA. Translation: BAB54037.1.
RefSeq	NP_107892.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1230547.
GenomeReviews	Gene locus mll7609 in contig BA000012_GR.
KEGG	mlo:mll7609.
NMPDR	fig|266835.1.peg.5993.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q985M5.
OMA	Q985M5. HMTVKNG.
Enzyme and pathway databases
BRENDA	1.1.99.1. 3315.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_RHILO

Accession

Primary (citable) accession number: Q985M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	October 1, 2001
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)