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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Nucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLOT266835:G1G20-6183-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:mlr7698
OrganismiMesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092711 – 986Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST986

Interactioni

Protein-protein interaction databases

STRINGi266835.mlr7698

Structurei

3D structure databases

ProteinModelPortaliQ985F6
SMRiQ985F6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 470Adenylyl removaseUniRule annotationAdd BLAST470
Regioni476 – 986Adenylyl transferaseUniRule annotationAdd BLAST511

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6 Bacteria
COG1391 LUCA
HOGENOMiHOG000256492
KOiK00982
OMAiFTVQLLQ
OrthoDBiPOG091H0675

Family and domain databases

HAMAPiMF_00802 GlnE, 1 hit
InterProiView protein in InterPro
IPR023057 GlnE
IPR005190 GlnE_rpt_dom
IPR018060 HTH_AraC
IPR013546 PII_UdlTrfase/GS_AdlTrfase
PANTHERiPTHR30621 PTHR30621, 1 hit
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 1 hit
PF03710 GlnE, 2 hits

Sequencei

Sequence statusi: Complete.

Q985F6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVAKRTVT DWQLKPALGL APLDQGRARQ ELAEIAAAAR EEGLARLAKF
60 70 80 90 100
LAGKGAGQDF LAAVFDLSPF LRDTSRRRPR ILDALFDQPV EARLEAITAA
110 120 130 140 150
IEQAPLAETV SESSLMMELR QCKAEAHFLI ALADLAGEAE TSLTVRRLSD
160 170 180 190 200
LADACTRAAV DFLLRDAHGQ GKLKLPDLDN PSLQSGWILL GMGKLGAHEL
210 220 230 240 250
NFSSDIDLVV FFDPDAPAVV DRLDATELFS RLTRRLVRIL QDRTEHGYVF
260 270 280 290 300
RTDLRLRPDP GSTPLAIPVE AALRYYEARG QNWERAAMIK ARPVAGDLAA
310 320 330 340 350
GAAFLRELQP YIWRKYMDYA AIADVHSIKR QIHAHKGHGE IAVKGHNVKL
360 370 380 390 400
GRGGIREIEF FVQTQQLIAG GRFPELRGRE TVPMLGQLAA RGWITADARD
410 420 430 440 450
ALARQYWFLR RVEHAVQMVA DEQTHTLPED DEGLERIAHM LGFGDAAAFS
460 470 480 490 500
QAFRASLLQV ERHYAALFET APELSAGIGN LVFTGDVDDP NTLQTLQGLG
510 520 530 540 550
FQRPSDICRV IRGWHFGRYR VTQSAEARER LTELTPALLK AFGQTRRADE
560 570 580 590 600
ALIRFDEFLA GLPAGIQLFS LLQSNPALLK LMATIMGAAP RLAAIITRRP
610 620 630 640 650
HVFDGLLDPA LLTELPDRVY LSARLSAFLE GDRAYEEVLD RLRIFASEQK
660 670 680 690 700
FLIGVRLLAG SIDPARAGRA FSDLADLTIE AALQAVTAEF AARHGTIAGG
710 720 730 740 750
VVSLLGMGKL GSRELTAGSD VDLILLYDHD ADAEDSDGDK PLAPSHYYTR
760 770 780 790 800
MTQRLISAVS APTAEGVLYE LDLRLRPSGN KGPVATHIDA FKKYQRQEAW
810 820 830 840 850
TWEHMALSRA RAIGGDTGLC AEVEAEVAAV LGQPRDAAKV KTEAADMRAM
860 870 880 890 900
IEKEKPARDL WDIKLIPGGL IDLEFIAQVA VITGQVGAGP RATGTAEILS
910 920 930 940 950
RLAPGFADAG ARQELGAAFA LYLALTQMTR LCLTGSFERD DVPPGLSDLL
960 970 980
LAVTDLPDFG VLEAHLKETS QKVRKDFDLL LRAGRP
Length:986
Mass (Da):107,857
Last modified:October 1, 2001 - v1
Checksum:iD3D040C276EE297C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA Translation: BAB54106.1
RefSeqiWP_010915054.1, NC_002678.2

Genome annotation databases

EnsemblBacteriaiBAB54106; BAB54106; BAB54106
KEGGimlo:mlr7698
PATRICifig|266835.9.peg.6160

Similar proteinsi

Entry informationi

Entry nameiGLNE_RHILO
AccessioniPrimary (citable) accession number: Q985F6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2001
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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