Reviewed, UniProtKB/Swiss-Prot Q985B1 (PYRE2_RHILO)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase 2
      Short name=OPRT 2
      Short name=OPRTase 2
    EC=2.4.2.10

Gene names

Name:	pyrE2
Ordered Locus Names:	mlr7756

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Orotate phosphoribosyltransferase 2 HAMAP MF_01208		PRO_0000110730
Regions
	Region	116 – 124	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	120	1	Orotate By similarity
	Binding site	148	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q985B1-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: ECB41C53A84D830A	FASTA	192	20,826
	10 20 30 40 50 60 MKTDEVLGIF REAGAVLEGH FILTSGLRSP VFLQKARVFM HADKTERLCR ALAEKIRAAV 70 80 90 100 110 120 PGKIDYVVGP AIGGLIPAYE TSRHLGVPAI WVEREGGEFR LRRFEIARGA RVVIVEDIVT 130 140 150 160 170 180 TGLSIRETIE CLRELGAEVV AAACIIDRSA GKTHVGVPLI ALAEYEVPAY PPDRLPPELA 190 AIPAVKPGSR NI

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000012 Genomic DNA. Translation: BAB54152.1.
RefSeq	NP_108007.1.
3D structure databases
HSSP	HSSP built from PDB template 1LH0 based on UniProtKB P08870.
ModBase	Search...
Genome annotation databases
GeneID	1230662.
GenomeReviews	Gene locus mlr7756 in contig BA000012_GR.
KEGG	mlo:mlr7756.
NMPDR	fig|266835.1.peg.6108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q985B1.
OMA	YLQSAKV.
Enzyme and pathway databases
BRENDA	2.4.2.10. 3315.
Family and domain databases
HAMAP	MF_01208. [Tree]
InterPro	IPR006273. Orotate_PRibTrfase_thermus-typ. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. pyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE2_RHILO

Accession

Primary (citable) accession number: Q985B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)