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Protein

DNA repair protein RadA

Gene

radA

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.UniRule annotation
Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 27C4-typeUniRule annotationAdd BLAST18
Nucleotide bindingi98 – 105ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repairUniRule annotation, Stress responseUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, DNA-bindingUniRule annotation, Metal-binding, Nucleotide-binding, Zinc

Protein family/group databases

MEROPSiS16.A04.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RadAUniRule annotation
Gene namesi
Name:radAUniRule annotation
Ordered Locus Names:mll7835Imported
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)Imported
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi266835.mll7835.

Structurei

3D structure databases

ProteinModelPortaliQ984U8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 219RECA_2InterPro annotationAdd BLAST151

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 467Lon-protease-likeUniRule annotationAdd BLAST113

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi256 – 260RadA KNRFG motifUniRule annotation5

Domaini

Has a putative N-terminal zinc-finger, a middle region with homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.UniRule annotation

Sequence similaritiesi

Belongs to the RecA family. RadA subfamily.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 27C4-typeUniRule annotationAdd BLAST18

Keywords - Domaini

Zinc-fingerUniRule annotation

Phylogenomic databases

eggNOGiENOG4105DNJ. Bacteria.
COG1066. LUCA.
HOGENOMiHOG000218329.
KOiK04485.
OMAiEVGCFEL.
OrthoDBiPOG091H02IA.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q984U8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSRVQFIC QNCGSVHQRW AGKCDACGEW NTLVEEGTSG GIGSGPANMR
60 70 80 90 100
NARKGRAVVL TTLSGDIEDA PRIISGIGEL DRATGGGFVR GSALLVGGDP
110 120 130 140 150
GIGKSTLLTQ AAAALASRGH RIVYVSGEEA VAQIRLRAQR LGVADSPVEL
160 170 180 190 200
AAETNVEDIL ATIADGKRPD LVILDSIQTL WTDLADSAPG TVTQVRAAAQ
210 220 230 240 250
AMIRYAKSTG AAIVLVGHVT KEGQIAGPRV VEHMVDGVLY FEGEGGHHYR
260 270 280 290 300
ILRTVKNRFG PTDEIGVFEM SDKGLREVSN PSELFLGERH AKSPGAAVFA
310 320 330 340 350
GMEGTRPVLV EIQALVAQSS LGTPRRAVVG WDGARLSMVL AVLEAHCGVR
360 370 380 390 400
FGQHDVYLNV AGGYRISEPA ADLAVAAALV SSLTGLALPA DCVYFGEISL
410 420 430 440 450
SGAVRPVAHA QQRLKEAEKL GFGSAVLPLG SEELAGGIGA GAFQPTELAD
460
LVARIAGSRR SRVDEEE
Length:467
Mass (Da):48,824
Last modified:October 1, 2001 - v1
Checksum:iE48D74ADB6E459D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB54215.1.
RefSeqiWP_010915159.1. NC_002678.2.

Genome annotation databases

EnsemblBacteriaiBAB54215; BAB54215; BAB54215.
GeneIDi1230722.
KEGGimlo:mll7835.
PATRICi22486791. VBIMesLot2464_6272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB54215.1.
RefSeqiWP_010915159.1. NC_002678.2.

3D structure databases

ProteinModelPortaliQ984U8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266835.mll7835.

Protein family/group databases

MEROPSiS16.A04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB54215; BAB54215; BAB54215.
GeneIDi1230722.
KEGGimlo:mll7835.
PATRICi22486791. VBIMesLot2464_6272.

Phylogenomic databases

eggNOGiENOG4105DNJ. Bacteria.
COG1066. LUCA.
HOGENOMiHOG000218329.
KOiK04485.
OMAiEVGCFEL.
OrthoDBiPOG091H02IA.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ984U8_RHILO
AccessioniPrimary (citable) accession number: Q984U8
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2001
Last sequence update: October 1, 2001
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.