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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189SubstrateUniRule annotation1
Active sitei190Proton donor/acceptorUniRule annotation1
Active sitei320UniRule annotation1
Binding sitei321SubstrateUniRule annotation1
Sitei333Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

BioCyciMLOT266835:G1G20-6569-MONOMER
UniPathwayiUPA00223; UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:mll8172
OrganismiMesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001613041 – 465Fumarate hydratase class IIAdd BLAST465

Proteomic databases

PRIDEiQ983U5

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi266835.mll8172

Structurei

3D structure databases

ProteinModelPortaliQ983U5
SMRiQ983U5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 102Substrate bindingUniRule annotation3
Regioni131 – 134Substrate binding (B site)UniRule annotation4
Regioni141 – 143Substrate bindingUniRule annotation3
Regioni326 – 328Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q Bacteria
COG0114 LUCA
HOGENOMiHOG000061736
KOiK01679
OMAiVSFTDNC
OrthoDBiPOG091H01XG

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q983U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVAKTRTET DTFGPIEVAA DRYWGAQAQR SLGNFKIGWE KQPASIVRAL
60 70 80 90 100
GVVKRAAAEV NMEMKRLDPV VGKAIVDAAQ EVIDGKLNDH FPLVVWQTGS
110 120 130 140 150
GTQSNMNANE VISNRAIEML GGVMGSKKPV HPNDHVNMSQ SSNDTYPTAM
160 170 180 190 200
HIACAEQVVH HLIPALHHLH KALDAKARAF NHIIKIGRTH TQDATPLTLG
210 220 230 240 250
QEFSGYAAQV ASSIKRIEMT LPGLQELAQG GTAVGTGLNA PVGFAEKVAD
260 270 280 290 300
RIAAITGIAF VTAPNKFEAL AAHDSMVFSH GAINAAAAAL FKIANDIRFL
310 320 330 340 350
GSGPRSGLGE LSLPENEPGS SIMPGKVNPT QCEAMTQVCV QVFGNNAALT
360 370 380 390 400
FAGSQGHFEL NVYNPLMAYN FLQSVQLLSD ASVSFTDNCV VGIEAREDNI
410 420 430 440 450
KAALDRSLML VTALAPTIGY DNAAKIAKTA HKNGTTLREE ALATGLVSEV
460
DYDRLVRPED MTHPG
Length:465
Mass (Da):49,592
Last modified:October 1, 2001 - v1
Checksum:i85407C084D9221F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA Translation: BAB53785.1
RefSeqiWP_010915411.1, NC_002678.2

Genome annotation databases

EnsemblBacteriaiBAB53785; BAB53785; BAB53785
KEGGimlo:mll8172
PATRICifig|266835.9.peg.6528

Similar proteinsi

Entry informationi

Entry nameiFUMC_RHILO
AccessioniPrimary (citable) accession number: Q983U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2001
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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