Reviewed, UniProtKB/Swiss-Prot Q983U5 (FUMC_RHILO)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	mll8172

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161304
Regions
	Region	131 – 134	4	B site By similarity
	Region	141 – 143	3	Substrate binding By similarity
Sites
	Binding site	102	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q983U5-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 85407C084D9221F9	FASTA	465	49,592
	10 20 30 40 50 60 MTVAKTRTET DTFGPIEVAA DRYWGAQAQR SLGNFKIGWE KQPASIVRAL GVVKRAAAEV 70 80 90 100 110 120 NMEMKRLDPV VGKAIVDAAQ EVIDGKLNDH FPLVVWQTGS GTQSNMNANE VISNRAIEML 130 140 150 160 170 180 GGVMGSKKPV HPNDHVNMSQ SSNDTYPTAM HIACAEQVVH HLIPALHHLH KALDAKARAF 190 200 210 220 230 240 NHIIKIGRTH TQDATPLTLG QEFSGYAAQV ASSIKRIEMT LPGLQELAQG GTAVGTGLNA 250 260 270 280 290 300 PVGFAEKVAD RIAAITGIAF VTAPNKFEAL AAHDSMVFSH GAINAAAAAL FKIANDIRFL 310 320 330 340 350 360 GSGPRSGLGE LSLPENEPGS SIMPGKVNPT QCEAMTQVCV QVFGNNAALT FAGSQGHFEL 370 380 390 400 410 420 NVYNPLMAYN FLQSVQLLSD ASVSFTDNCV VGIEAREDNI KAALDRSLML VTALAPTIGY 430 440 450 460 DNAAKIAKTA HKNGTTLREE ALATGLVSEV DYDRLVRPED MTHPG

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000012 Genomic DNA. Translation: BAB53785.1.
RefSeq	NP_108324.1.
3D structure databases
HSSP	HSSP built from PDB template 1YFM based on UniProtKB P08417.
ModBase	Search...
Genome annotation databases
GeneID	1230976.
GenomeReviews	Gene locus mll8172 in contig BA000012_GR.
KEGG	mlo:mll8172.
NMPDR	fig|266835.1.peg.6423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q983U5.
OMA	GSQGHFE.
Enzyme and pathway databases
BRENDA	4.2.1.2. 3315.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_RHILO

Accession

Primary (citable) accession number: Q983U5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)