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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei621Aminoacyl-adenylateUniRule annotation1
Binding sitei665ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:mlr8250
OrganismiMesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi
  • UP000000552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984531 – 993Isoleucine--tRNA ligaseAdd BLAST993

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266835.mlr8250

Structurei

3D structure databases

ProteinModelPortaliQ983N4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi64 – 74"HIGH" regionAdd BLAST11
Motifi662 – 666"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q983N4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTVETIDY SKTLYLPQTD FPMRAGLPEK EPVLVKRWQD MDLYAKLRES
60 70 80 90 100
AAGRTKYVLH DGPPYANGNI HIGHALNKIL KDVITRSFQM RGYDSTYVPG
110 120 130 140 150
WDCHGLPIEW KIEEQYRAKG KNKDEVPVNE FRKECREFAA HWITVQGGEF
160 170 180 190 200
QRLGVIGDFK NPYTTMAFHA ESRIAGELLK FALSGQLYRG SKPVMWSVVE
210 220 230 240 250
RTALAEAEIE YQDYESDTIW AKFPVANLVV ANVVDGQPAE LNPDLSDRSL
260 270 280 290 300
DLLDAHVVIW TTTPWTIPGN RAVSYSPRVA YGLYEVTAAE NAFGPEPGEK
310 320 330 340 350
LIFADALAAE SQAKAKITLK RLHHVSAEQL GNLVLSHPFK GLGGGYEFPV
360 370 380 390 400
PMVAGDHVTD DAGTGFVHTA PGHGREDFDA WMDAAPQLRA RGIDTVIPFT
410 420 430 440 450
VDDAGFFTRD APGFGPDREG GAARVIDDNG KKGNANQAVI DELIKRNALF
460 470 480 490 500
ARGRLKHSYP HSWRSKKPVI FRNTPQWFVY MDKDLGDGTT LRSRALKAID
510 520 530 540 550
DTRFVPAAGQ NRIRAMIEER PDWVLSRQRA WGVPIAVFAD EDGNVLKDEA
560 570 580 590 600
VNQRIMDAFE EEGADAWFAA GAKERFLGNH DASKWKQVMD ILDVWFDSGS
610 620 630 640 650
THVFTLEDRP DLKWPADVYL EGSDQHRGWF HSSLLESCGT RGRAPYDTVV
660 670 680 690 700
THGFTMDEDG RKMSKSLGNT VVPQDVIKQS GADILRLWVV TTDYWEDQRL
710 720 730 740 750
GKNVLQTNID AYRKLRNTIR WMLGTLAHDD GETVPLEAMP ELERLMLHRL
760 770 780 790 800
AELDEVVRQG YDAFEFKRIT RALVDFMVVE LSAFYFDIRK DALYCDAPSS
810 820 830 840 850
VKRKASVQVV RHLFDCLVKW LAPMLPFTME EAWLDRHPDA VSVHLDQFPE
860 870 880 890 900
IPADWKNEAL AEKWRKVRQV RRVVTGALEI ARAQKVIGSS LEAVPVVTIN
910 920 930 940 950
DAALEAAIAD VDMAEMAITS DLVIAHGQAP EGAFTLDDVR GVAVVVEKAE
960 970 980 990
DRGLVKCARS WRYTADVGQD PAFPDVSARD AAVLHELKAL GRL
Length:993
Mass (Da):111,343
Last modified:December 20, 2005 - v2
Checksum:iF99288C8BF91E089
GO

Sequence cautioni

The sequence BAB53846 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA Translation: BAB53846.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiBAB53846; BAB53846; BAB53846
KEGGimlo:mlr8250

Similar proteinsi

Entry informationi

Entry nameiSYI_RHILO
AccessioniPrimary (citable) accession number: Q983N4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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