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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei621 – 6211Aminoacyl-adenylateUniRule annotation
Binding sitei665 – 6651ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLOT266835:GJ9L-6539-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:mlr8250
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
ProteomesiUP000000552 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 993993Isoleucine--tRNA ligasePRO_0000098453Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266835.mlr8250.

Structurei

3D structure databases

ProteinModelPortaliQ983N4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionAdd
BLAST
Motifi662 – 6665"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q983N4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTVETIDY SKTLYLPQTD FPMRAGLPEK EPVLVKRWQD MDLYAKLRES
60 70 80 90 100
AAGRTKYVLH DGPPYANGNI HIGHALNKIL KDVITRSFQM RGYDSTYVPG
110 120 130 140 150
WDCHGLPIEW KIEEQYRAKG KNKDEVPVNE FRKECREFAA HWITVQGGEF
160 170 180 190 200
QRLGVIGDFK NPYTTMAFHA ESRIAGELLK FALSGQLYRG SKPVMWSVVE
210 220 230 240 250
RTALAEAEIE YQDYESDTIW AKFPVANLVV ANVVDGQPAE LNPDLSDRSL
260 270 280 290 300
DLLDAHVVIW TTTPWTIPGN RAVSYSPRVA YGLYEVTAAE NAFGPEPGEK
310 320 330 340 350
LIFADALAAE SQAKAKITLK RLHHVSAEQL GNLVLSHPFK GLGGGYEFPV
360 370 380 390 400
PMVAGDHVTD DAGTGFVHTA PGHGREDFDA WMDAAPQLRA RGIDTVIPFT
410 420 430 440 450
VDDAGFFTRD APGFGPDREG GAARVIDDNG KKGNANQAVI DELIKRNALF
460 470 480 490 500
ARGRLKHSYP HSWRSKKPVI FRNTPQWFVY MDKDLGDGTT LRSRALKAID
510 520 530 540 550
DTRFVPAAGQ NRIRAMIEER PDWVLSRQRA WGVPIAVFAD EDGNVLKDEA
560 570 580 590 600
VNQRIMDAFE EEGADAWFAA GAKERFLGNH DASKWKQVMD ILDVWFDSGS
610 620 630 640 650
THVFTLEDRP DLKWPADVYL EGSDQHRGWF HSSLLESCGT RGRAPYDTVV
660 670 680 690 700
THGFTMDEDG RKMSKSLGNT VVPQDVIKQS GADILRLWVV TTDYWEDQRL
710 720 730 740 750
GKNVLQTNID AYRKLRNTIR WMLGTLAHDD GETVPLEAMP ELERLMLHRL
760 770 780 790 800
AELDEVVRQG YDAFEFKRIT RALVDFMVVE LSAFYFDIRK DALYCDAPSS
810 820 830 840 850
VKRKASVQVV RHLFDCLVKW LAPMLPFTME EAWLDRHPDA VSVHLDQFPE
860 870 880 890 900
IPADWKNEAL AEKWRKVRQV RRVVTGALEI ARAQKVIGSS LEAVPVVTIN
910 920 930 940 950
DAALEAAIAD VDMAEMAITS DLVIAHGQAP EGAFTLDDVR GVAVVVEKAE
960 970 980 990
DRGLVKCARS WRYTADVGQD PAFPDVSARD AAVLHELKAL GRL
Length:993
Mass (Da):111,343
Last modified:December 19, 2005 - v2
Checksum:iF99288C8BF91E089
GO

Sequence cautioni

The sequence BAB53846.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB53846.1. Different initiation.
RefSeqiNP_108385.1. NC_002678.2.

Genome annotation databases

EnsemblBacteriaiBAB53846; BAB53846; BAB53846.
GeneIDi1231037.
KEGGimlo:mlr8250.
PATRICi22487435. VBIMesLot2464_6591.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB53846.1. Different initiation.
RefSeqiNP_108385.1. NC_002678.2.

3D structure databases

ProteinModelPortaliQ983N4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266835.mlr8250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB53846; BAB53846; BAB53846.
GeneIDi1231037.
KEGGimlo:mlr8250.
PATRICi22487435. VBIMesLot2464_6591.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciMLOT266835:GJ9L-6539-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MAFF303099.

Entry informationi

Entry nameiSYI_RHILO
AccessioniPrimary (citable) accession number: Q983N4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 19, 2005
Last sequence update: December 19, 2005
Last modified: January 6, 2015
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.