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Q983N4

- SYI_RHILO

UniProt

Q983N4 - SYI_RHILO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei621 – 6211Aminoacyl-adenylateUniRule annotation
    Binding sitei665 – 6651ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMLOT266835:GJ9L-6539-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:mlr8250
    OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
    Taxonomic identifieri266835 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
    ProteomesiUP000000552: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 993993Isoleucine--tRNA ligasePRO_0000098453Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi266835.mlr8250.

    Structurei

    3D structure databases

    ProteinModelPortaliQ983N4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 7411"HIGH" regionAdd
    BLAST
    Motifi662 – 6665"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q983N4-1 [UniParc]FASTAAdd to Basket

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    MTDTVETIDY SKTLYLPQTD FPMRAGLPEK EPVLVKRWQD MDLYAKLRES    50
    AAGRTKYVLH DGPPYANGNI HIGHALNKIL KDVITRSFQM RGYDSTYVPG 100
    WDCHGLPIEW KIEEQYRAKG KNKDEVPVNE FRKECREFAA HWITVQGGEF 150
    QRLGVIGDFK NPYTTMAFHA ESRIAGELLK FALSGQLYRG SKPVMWSVVE 200
    RTALAEAEIE YQDYESDTIW AKFPVANLVV ANVVDGQPAE LNPDLSDRSL 250
    DLLDAHVVIW TTTPWTIPGN RAVSYSPRVA YGLYEVTAAE NAFGPEPGEK 300
    LIFADALAAE SQAKAKITLK RLHHVSAEQL GNLVLSHPFK GLGGGYEFPV 350
    PMVAGDHVTD DAGTGFVHTA PGHGREDFDA WMDAAPQLRA RGIDTVIPFT 400
    VDDAGFFTRD APGFGPDREG GAARVIDDNG KKGNANQAVI DELIKRNALF 450
    ARGRLKHSYP HSWRSKKPVI FRNTPQWFVY MDKDLGDGTT LRSRALKAID 500
    DTRFVPAAGQ NRIRAMIEER PDWVLSRQRA WGVPIAVFAD EDGNVLKDEA 550
    VNQRIMDAFE EEGADAWFAA GAKERFLGNH DASKWKQVMD ILDVWFDSGS 600
    THVFTLEDRP DLKWPADVYL EGSDQHRGWF HSSLLESCGT RGRAPYDTVV 650
    THGFTMDEDG RKMSKSLGNT VVPQDVIKQS GADILRLWVV TTDYWEDQRL 700
    GKNVLQTNID AYRKLRNTIR WMLGTLAHDD GETVPLEAMP ELERLMLHRL 750
    AELDEVVRQG YDAFEFKRIT RALVDFMVVE LSAFYFDIRK DALYCDAPSS 800
    VKRKASVQVV RHLFDCLVKW LAPMLPFTME EAWLDRHPDA VSVHLDQFPE 850
    IPADWKNEAL AEKWRKVRQV RRVVTGALEI ARAQKVIGSS LEAVPVVTIN 900
    DAALEAAIAD VDMAEMAITS DLVIAHGQAP EGAFTLDDVR GVAVVVEKAE 950
    DRGLVKCARS WRYTADVGQD PAFPDVSARD AAVLHELKAL GRL 993
    Length:993
    Mass (Da):111,343
    Last modified:December 20, 2005 - v2
    Checksum:iF99288C8BF91E089
    GO

    Sequence cautioni

    The sequence BAB53846.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000012 Genomic DNA. Translation: BAB53846.1. Different initiation.
    RefSeqiNP_108385.1. NC_002678.2.

    Genome annotation databases

    EnsemblBacteriaiBAB53846; BAB53846; BAB53846.
    GeneIDi1231037.
    KEGGimlo:mlr8250.
    PATRICi22487435. VBIMesLot2464_6591.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000012 Genomic DNA. Translation: BAB53846.1 . Different initiation.
    RefSeqi NP_108385.1. NC_002678.2.

    3D structure databases

    ProteinModelPortali Q983N4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266835.mlr8250.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB53846 ; BAB53846 ; BAB53846 .
    GeneIDi 1231037.
    KEGGi mlo:mlr8250.
    PATRICi 22487435. VBIMesLot2464_6591.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MLOT266835:GJ9L-6539-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MAFF303099.

    Entry informationi

    Entry nameiSYI_RHILO
    AccessioniPrimary (citable) accession number: Q983N4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3