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Protein

Homogentisate 1,2-dioxygenase

Gene

hmgA

Organism
Rhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.UniRule annotation

Catalytic activityi

Homogentisate + O2 = 4-maleylacetoacetate.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei308 – 3081Proton acceptorUniRule annotation
Metal bindingi351 – 3511IronUniRule annotation
Metal bindingi357 – 3571IronUniRule annotation
Binding sitei366 – 3661homogentisateUniRule annotation
Metal bindingi387 – 3871IronUniRule annotation
Binding sitei387 – 3871homogentisateUniRule annotation

GO - Molecular functioni

  1. homogentisate 1,2-dioxygenase activity Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-phenylalanine catabolic process Source: UniProtKB-HAMAP
  2. tyrosine catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMLOT266835:GJ9L-6580-MONOMER.
UniPathwayiUPA00139; UER00339.

Names & Taxonomyi

Protein namesi
Recommended name:
Homogentisate 1,2-dioxygenaseUniRule annotation (EC:1.13.11.5UniRule annotation)
Short name:
HGDOUniRule annotation
Alternative name(s):
Homogentisate oxygenaseUniRule annotation
Homogentisic acid oxidaseUniRule annotation
HomogentisicaseUniRule annotation
Gene namesi
Name:hmgAUniRule annotation
Ordered Locus Names:mlr8303
OrganismiRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
ProteomesiUP000000552: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Homogentisate 1,2-dioxygenasePRO_0000220253Add
BLAST

Interactioni

Subunit structurei

Hexamer; dimer of trimers.UniRule annotation

Protein-protein interaction databases

STRINGi266835.mlr8303.

Structurei

3D structure databases

ProteinModelPortaliQ983J4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the homogentisate dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q983J4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNTGRLGFV KLGAGIKEDR MAFSYMPGFG NDFETETLPG SLPQGRNSPQ
60 70 80 90 100
RPAYGLYAEQ LSGSPFTAPR GTNERSWLYR IRPSVKHTGR FKAASYPLWK
110 120 130 140 150
TAPNVGDHEL ALGQYRWNPV PMPKEPTDFI QGMRSITTAG DVLGQTGMAA
160 170 180 190 200
HVYVANRSMV DDHFFNADGE LLVVPQVGAL RFVTEMGVIE LRPGEIAVLP
210 220 230 240 250
RGLVFKAELV DKDVRGYVCE NYGAKLTLPD RGPIGANCLA NPRDFKTPCA
260 270 280 290 300
WFEEKETPCR LIVKWCGNFH VTEIGHSPLD VVAWHGNYAP YKYDLATFSP
310 320 330 340 350
VGAILFDHPD PSIFTVLTAP SGEEGTANID FVIFPPRWLV AEDTFRPPWY
360 370 380 390 400
HRNIMSEFMG LIHGQYDAKE EGFVPGGISL HNLMLAHGPD APGFEKASRA
410 420 430 440 450
DLKPVKLDNT MAFMFETRFP QMLTRYGAEL ETRQDNYIDC WADLKKRFNG

TPEGDWS
Length:457
Mass (Da):51,046
Last modified:October 1, 2001 - v1
Checksum:i6A20B69E9A2B2BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB53887.1.
RefSeqiNP_108426.1. NC_002678.2.
WP_010915513.1. NC_002678.2.

Genome annotation databases

EnsemblBacteriaiBAB53887; BAB53887; BAB53887.
GeneIDi1231078.
KEGGimlo:mlr8303.
PATRICi22487519. VBIMesLot2464_6633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA. Translation: BAB53887.1.
RefSeqiNP_108426.1. NC_002678.2.
WP_010915513.1. NC_002678.2.

3D structure databases

ProteinModelPortaliQ983J4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266835.mlr8303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB53887; BAB53887; BAB53887.
GeneIDi1231078.
KEGGimlo:mlr8303.
PATRICi22487519. VBIMesLot2464_6633.

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339.
BioCyciMLOT266835:GJ9L-6580-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MAFF303099.

Entry informationi

Entry nameiHGD_RHILO
AccessioniPrimary (citable) accession number: Q983J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: October 1, 2001
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.