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Protein

Homogentisate 1,2-dioxygenase

Gene

hmgA

Organism
Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.UniRule annotation

Catalytic activityi

Homogentisate + O2 = 4-maleylacetoacetate.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi: L-phenylalanine degradation

This protein is involved in step 4 of the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Phenylalanine-4-hydroxylase (phhA)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Homogentisate 1,2-dioxygenase (hmgA)
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei308Proton acceptorUniRule annotation1
Metal bindingi351IronUniRule annotation1
Metal bindingi357IronUniRule annotation1
Binding sitei366homogentisateUniRule annotation1
Metal bindingi387IronUniRule annotation1
Binding sitei387homogentisateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processPhenylalanine catabolism, Tyrosine catabolism
LigandIron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339

Names & Taxonomyi

Protein namesi
Recommended name:
Homogentisate 1,2-dioxygenaseUniRule annotation (EC:1.13.11.5UniRule annotation)
Short name:
HGDOUniRule annotation
Alternative name(s):
Homogentisate oxygenaseUniRule annotation
Homogentisic acid oxidaseUniRule annotation
HomogentisicaseUniRule annotation
Gene namesi
Name:hmgAUniRule annotation
Ordered Locus Names:mlr8303
OrganismiMesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099))
Taxonomic identifieri266835 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002202531 – 457Homogentisate 1,2-dioxygenaseAdd BLAST457

Interactioni

Subunit structurei

Hexamer; dimer of trimers.UniRule annotation

Protein-protein interaction databases

STRINGi266835.mlr8303

Structurei

3D structure databases

ProteinModelPortaliQ983J4
SMRiQ983J4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the homogentisate dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F1J Bacteria
COG3508 LUCA
HOGENOMiHOG000139824
KOiK00451
OMAiHGNYTPY
OrthoDBiPOG091H04E0

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_00334 Homogentis_dioxygen, 1 hit
InterProiView protein in InterPro
IPR005708 Homogentis_dOase
IPR022950 Homogentis_dOase_bac
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR11056 PTHR11056, 1 hit
PfamiView protein in Pfam
PF04209 HgmA, 1 hit
SUPFAMiSSF51182 SSF51182, 1 hit
TIGRFAMsiTIGR01015 hmgA, 1 hit

Sequencei

Sequence statusi: Complete.

Q983J4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNTGRLGFV KLGAGIKEDR MAFSYMPGFG NDFETETLPG SLPQGRNSPQ
60 70 80 90 100
RPAYGLYAEQ LSGSPFTAPR GTNERSWLYR IRPSVKHTGR FKAASYPLWK
110 120 130 140 150
TAPNVGDHEL ALGQYRWNPV PMPKEPTDFI QGMRSITTAG DVLGQTGMAA
160 170 180 190 200
HVYVANRSMV DDHFFNADGE LLVVPQVGAL RFVTEMGVIE LRPGEIAVLP
210 220 230 240 250
RGLVFKAELV DKDVRGYVCE NYGAKLTLPD RGPIGANCLA NPRDFKTPCA
260 270 280 290 300
WFEEKETPCR LIVKWCGNFH VTEIGHSPLD VVAWHGNYAP YKYDLATFSP
310 320 330 340 350
VGAILFDHPD PSIFTVLTAP SGEEGTANID FVIFPPRWLV AEDTFRPPWY
360 370 380 390 400
HRNIMSEFMG LIHGQYDAKE EGFVPGGISL HNLMLAHGPD APGFEKASRA
410 420 430 440 450
DLKPVKLDNT MAFMFETRFP QMLTRYGAEL ETRQDNYIDC WADLKKRFNG

TPEGDWS
Length:457
Mass (Da):51,046
Last modified:October 1, 2001 - v1
Checksum:i6A20B69E9A2B2BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000012 Genomic DNA Translation: BAB53887.1

Genome annotation databases

EnsemblBacteriaiBAB53887; BAB53887; BAB53887
KEGGimlo:mlr8303

Entry informationi

Entry nameiHGD_RHILO
AccessioniPrimary (citable) accession number: Q983J4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: October 1, 2001
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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