Reviewed, UniProtKB/Swiss-Prot Q983J4 (HGD_RHILO)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	mlr8303

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity. HAMAP MF_00334
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_0000220253
Sites
	Metal binding	351	1	Iron By similarity
	Metal binding	357	1	Iron By similarity
	Metal binding	387	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q983J4-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 6A20B69E9A2B2BD1	FASTA	457	51,046
	10 20 30 40 50 60 MVNTGRLGFV KLGAGIKEDR MAFSYMPGFG NDFETETLPG SLPQGRNSPQ RPAYGLYAEQ 70 80 90 100 110 120 LSGSPFTAPR GTNERSWLYR IRPSVKHTGR FKAASYPLWK TAPNVGDHEL ALGQYRWNPV 130 140 150 160 170 180 PMPKEPTDFI QGMRSITTAG DVLGQTGMAA HVYVANRSMV DDHFFNADGE LLVVPQVGAL 190 200 210 220 230 240 RFVTEMGVIE LRPGEIAVLP RGLVFKAELV DKDVRGYVCE NYGAKLTLPD RGPIGANCLA 250 260 270 280 290 300 NPRDFKTPCA WFEEKETPCR LIVKWCGNFH VTEIGHSPLD VVAWHGNYAP YKYDLATFSP 310 320 330 340 350 360 VGAILFDHPD PSIFTVLTAP SGEEGTANID FVIFPPRWLV AEDTFRPPWY HRNIMSEFMG 370 380 390 400 410 420 LIHGQYDAKE EGFVPGGISL HNLMLAHGPD APGFEKASRA DLKPVKLDNT MAFMFETRFP 430 440 450 QMLTRYGAEL ETRQDNYIDC WADLKKRFNG TPEGDWS

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB53887.1.
RefSeq	NP_108426.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1231078.
GenomeReviews	Gene locus mlr8303 in contig BA000012_GR.
KEGG	mlo:mlr8303.
NMPDR	fig|266835.1.peg.6525.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG293508.
OMA	RNCMSEF.
Enzyme and pathway databases
BRENDA	1.13.11.5. 3315.
Family and domain databases
HAMAP	MF_00334. Homogentis_dioxygen. [Tree]
InterPro	IPR011051. Cupin_RmlC_type. IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_RHILO

Accession

Primary (citable) accession number: Q983J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)