Reviewed, UniProtKB/Swiss-Prot Q983A3 (HPPA_RHILO)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	mlr8418

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217024
Regions
	Transmembrane	2 – 21	20	Potential
	Transmembrane	50 – 72	23	Potential
	Transmembrane	77 – 99	23	Potential
	Transmembrane	124 – 146	23	Potential
	Transmembrane	158 – 180	23	Potential
	Transmembrane	235 – 257	23	Potential
	Transmembrane	259 – 281	23	Potential
	Transmembrane	296 – 318	23	Potential
	Transmembrane	334 – 356	23	Potential
	Transmembrane	378 – 400	23	Potential
	Transmembrane	407 – 429	23	Potential
	Transmembrane	472 – 489	18	Potential
	Transmembrane	521 – 543	23	Potential
	Transmembrane	588 – 610	23	Potential
	Transmembrane	617 – 639	23	Potential
	Transmembrane	688 – 710	23	Potential
Sites
	Site	467	1	Determinant of potassium independence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q983A3-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 2E349A1DAC13BF65	FASTA	713	72,496
	10 20 30 40 50 60 MTILYAVIAC GLLSVLYAIW ATRSVLASDQ GNARMQEISA AIREGAQAYL ARQYTTIALV 70 80 90 100 110 120 GVVVLLLAWW LLSITAAIGF LIGAVLSGAA GFIGMHVSVR ANVRTAQAAS NSLAAGLDIA 130 140 150 160 170 180 FKSGAITGML VAGLALLGVS IYYLILTGPM GLQPNDRIVI DSLVALGFGA SLISIFARLG 190 200 210 220 230 240 GGIFTKGADV GGDLVGKVEA GIPEDDPRNP ATIADNVGDN VGDCAGMAAD LFETYAVTVV 250 260 270 280 290 300 ATMVLGAIFF GGTAVLGAAM LYPLAICGAC ILTSIVGTFF VKLGANGSIM GALYKGLIVT 310 320 330 340 350 360 GLLSIVGLAV ATSVCFGWGE IGTVAGMVIT GKNLFICGLI GLAVTGLIVV ITEYYTGTNK 370 380 390 400 410 420 RPVNSIAQAS VTGHGTNVIQ GLAVSLESTA LPAIVIVGGI ISTYQLAGLY GTAIAVTTML 430 440 450 460 470 480 GLAGMIVALD AFGPVTDNAG GIAEMAGLPK EVRHSTDALD AVGNTTKAVT KGYAIGSAGL 490 500 510 520 530 540 GALVLFAAYS NDLKFFAANG DKYPYFQGMG EISFDLSNPY VVAGLIFGGL IPYLFGGIAM 550 560 570 580 590 600 TAVGRAAGAI VEEVRKQFRE DPGIMAGTSK PNYARAVDLL TKAAIREMII PSLLPVLAPL 610 620 630 640 650 660 VVYFGVLLIS GSKASAFAAL GASLLGVIVN GLFVAISMTS GGGAWDNAKK SFEDGFTDKD 670 680 690 700 710 GVKHLKGSEA HKASVTGDTV GDPYKDTAGP AVNPAIKITN IVALLLLAVL AHG

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000012 Genomic DNA. Translation: BAB54303.1.
RefSeq	NP_108517.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1231169.
GenomeReviews	Gene locus mlr8418 in contig BA000012_GR.
KEGG	mlo:mlr8418.
NMPDR	fig|266835.1.peg.6616.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q983A3.
OMA	PYLFGGI.
Enzyme and pathway databases
BRENDA	3.6.1.1. 3315.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_RHILO

Accession

Primary (citable) accession number: Q983A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)