Q98267 (NTP1_MCV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside triphosphatase I
EC=3.6.1.15

Alternative name(s):
NPH-I
Nucleoside triphosphate phosphohydrolase I
Short name=NPH I

Gene names

Name:	NPH1
Ordered Locus Names:	MC100L

Organism

Molluscum contagiosum virus subtype 1 (MOCV) (MCVI) [Reference proteome]

Taxonomic identifier

10280 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Molluscipoxvirus › Molluscum contagiosum virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent ATPase required for providing the needed energy to achieve the termination of early transcripts. Acts in concert with the RAP94 subunit of the virion RNA polymerase and the capping enzyme/VTF to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex. NPH-I must bind ssDNA in order to exhibit ATPase activity By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Monomer. Interacts (via C-terminus) with RAP94 (via N-terminus). Interacts with the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity.
Subcellular location	Virion By similarity. Note: Virion core enzyme By similarity.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 634	634	Nucleoside triphosphatase I		PRO_0000099104
Regions
	Domain	41 – 203	163	Helicase ATP-binding
	Domain	355 – 531	177	Helicase C-terminal
	Nucleotide binding	54 – 61	8	ATP By similarity
	Region	456 – 523	68	Binding to the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity
	Motif	140 – 143	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	Q98267 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B786EFBA17E12F56	FASTA	634	72,103
	10 20 30 40 50 60 MNLHAAYIDY ALRRSQHMQA EMSGADNVLL KDYQLFVAKV FLGLDSMNSL LLFQETGVGK 70 80 90 100 110 120 TVTAVYMLKH LRFLYTSWTV LVLVKKALVE EPWMNTILRF APEVAKDCVF MNYDDQNFHN 130 140 150 160 170 180 KFFTNIKTVS ARSRIFVIID ECHNFISKSL SREDGRARNT KHVYNFLARH IASSHNKLLC 190 200 210 220 230 240 LSATPIVNNV REFALLVNLL RPGVLPPQSL FHNKRLLDEA ELVSKLGCIC SYLVHHEASI 250 260 270 280 290 300 FEDVEGSELF ARKRVLLKYV RMSAKQEDIY HKARAAEFRS GIAVFRVHRR MAATFAFDEF 310 320 330 340 350 360 PVRKNLTPDE YDALVASLVR DFEALFAGRA LSDATRARLR AGEPIECAAS AEDLSLYQAL 370 380 390 400 410 420 YEHSCKYAEV CVAILASPGK CLVFEPFINL SGIRIFVKYL EVFGISYIEF SSRTKDTRTR 430 440 450 460 470 480 AVAEFNRVEN TNGELIKTCV FSLSGNEGIS FLSINDIFIL DMTWNEASLK QIIGRAIRLH 490 500 510 520 530 540 SHANNPPERR YVNVHFVVAQ LGSGAPSVDD DLLEIIQNKA REFSQLYRVL KLASIEWIHQ 550 560 570 580 590 600 HCREFAPVDD EAGFRALCSR AIDVTRGAST RRALATGENI WYAFSTLMIT VHPGFKTEDG 610 620 630 RVYDADGNFL TTMPERPIVR VQGTRLVYIF PELR

References

[1]	"Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes." Senkevich T.G., Bugert J.J., Sisler J.R., Koonin E.V., Darai G., Moss B. Science 273:813-816(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60315 Genomic DNA. Translation: AAC55228.1.
PIR	T30702.
RefSeq	NP_044051.1. NC_001731.1.
3D structure databases
ProteinModelPortal	Q98267.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1487119.
Phylogenomic databases
ProtClustDB	CLSP2509799.
Family and domain databases
InterPro	IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_MCV1

Accession

Primary (citable) accession number: Q98267

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program