Q98218 (NPH2_MCV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA helicase NPH-II
EC=3.6.4.13

Alternative name(s):
Nucleoside triphosphatase II
Short name=NTPase II
Nucleoside triphosphate phosphohydrolase II
Short name=NPH II

Gene names

Name:	NPH2
ORF Names:	50R

Organism

Molluscum contagiosum virus subtype 1 (MOCV) (MCVI)

Taxonomic identifier

10280 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Molluscipoxvirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NTP-dependent helicase that catalyzes unidirectional unwinding of 3'tailed duplex RNAs and plays an important role during transcription of early mRNAs, presumably by preventing R-loop formation behind the elongating RNA polymerase. Might also play a role in the export of newly synthesized mRNA chains out of the core into the cytoplasm. Required for replication and propagation of viral particles By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Monomer.
Subcellular location	Virion. Note: Localizes to the virion core By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 684	684	RNA helicase NPH-II		PRO_0000055188
Regions
	Domain	184 – 359	176	Helicase ATP-binding
	Domain	392 – 563	172	Helicase C-terminal
	Nucleotide binding	197 – 204	8	ATP By similarity
	Motif	308 – 311	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	Q98218 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 51072B193CCC7284	FASTA	684	76,557
	10 20 30 40 50 60 MKNLPGVFAF PDNETIFAHA YSQRELEAML PARAGAQPHA FAYAAWPLSK HRWRGAFVCR 70 80 90 100 110 120 QRGVLKLNLE LDAGAFERVP RSEAERFEPE QSDARALMHR GADGTVMSFE CYSFLRCRPG 130 140 150 160 170 180 LELREAGLAL LRGLVVGGNR MRIFSGVRRA GASAATSAGM LGNESPFERA PFASLRIDAQ 190 200 210 220 230 240 AALFRAWAAR RPTVVTGGTG VGKTSQVPKL LLWFNYLFGG TEDLDVLAPA RERPVVLSLP 250 260 270 280 290 300 RVALVRMNGH ALRRALGFDA LEGSPVELRY GDLAPADANS SRSPFRLVVS TNQLTLGALF 310 320 330 340 350 360 AHGTVILDEV HEHDQMADIM LAVLRVHRAR VDSIVLMSAT LEDDRERLQE FFPDAEFVHI 370 380 390 400 410 420 PGSTRFEIRG VYVRNSSDPR DARAYDEEER RNVSAALSAH RPRAGRCGIL FVASVAQCED 430 440 450 460 470 480 YARLLTREHP ELGVYVVHGK TPNVDALLAE VYASARPCVL VSTPYLESSV TIRTVTHVYD 490 500 510 520 530 540 TGRVFVPAPF GGRQMLISPA MRTQRRGRVG RVMPGTYVYF YDPARLAPIK RIDSEFLYNY 550 560 570 580 590 600 IIYARHYGLV LPDDLYVQPS DLELLRRCEE YLDGFGLAPE RLFELASTRY LRMVEYAKIY 610 620 630 640 650 660 ARGGARAEEL NRFERDGVVT EDVLASIRAL NLRARVLSAR ARRGQFLHTC EVAFGPYAGT 670 680 RFLLANRRRL RGDIFMVTER SFVL

References

[1]	"Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes." Senkevich T.G., Bugert J.J., Sisler J.R., Koonin E.V., Darai G., Moss B. Science 273:813-816(1996) [PubMed: 8670425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60315 Genomic DNA. Translation: AAC55178.1.
PIR	T30652.
RefSeq	NP_044001.1. NC_001731.1.
3D structure databases
ProteinModelPortal	Q98218.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1670235.
Phylogenomic databases
ProtClustDB	PHA2653.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR001650. Helicase_C. IPR021892. NPH-II. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF12011. DUF3503. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NPH2_MCV1

Accession

Primary (citable) accession number: Q98218

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program