Q981V0 (ARLY2_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate lyase 2
Short name=ASAL 2
EC=4.3.2.1

Alternative name(s):
Arginosuccinase 2

Gene names

Name:	argH2
Ordered Locus Names:	mll9226

Encoded on

Plasmid pMLa

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	927 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. HAMAP MF_00006
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3. HAMAP MF_00006
Subcellular location	Cytoplasm By similarity HAMAP MF_00006.
Sequence similarities	In the C-terminal section; belongs to the lyase 1 family. Argininosuccinate lyase subfamily. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	arginine biosynthetic process via ornithine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro argininosuccinate lyase activity Inferred from electronic annotation. Source: EC ligase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 927	927	Argininosuccinate lyase 2 HAMAP MF_00006		PRO_0000137811
Regions
	Domain	135 – 333	199	ATP-grasp
	Region	431 – 927	497	Argininosuccinate lyase HAMAP MF_00006

Sequences

	Sequence		Length	Mass (Da)
	Q981V0 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 64E4255459968AB5	FASTA	927	99,126
	10 20 30 40 50 60 MEPIPLEAEK PGSCPEPFGG SLMNRGSFLF VESNTTGTGE LLMKRARLLG FETYLVTRNP 70 80 90 100 110 120 ARYPFLRDSV AQLIEAETRS PDELVGVATK LSGLAGIYSS SDYFVEAASS AAMAMGLPAA 130 140 150 160 170 180 NARAIATCRN KWKQATELQR HSIPIPETWL ATSIRDVENI LAHGTLPVVV KPVSGSGSSG 190 200 210 220 230 240 VRLCDSAAAA IAGFNSAKSV LQDQVDLPSP DILIQQYVEG KEYSAEIIAY DGKLHCLGIL 250 260 270 280 290 300 AKHKGPPPCF VEVGHDFPAP LLEPSLQELA SFASGAVSAL GLEFGPAHVE FVITESGPVI 310 320 330 340 350 360 IEVNPRLAGG MIPVMLSHAL GTSILDMVIR LYAGEGFTPP RGSARAGAIR FRLAPTSGKL 370 380 390 400 410 420 KSLGFSRNPD PTVPEAGLLK SEGGDVQIKG DFRDRVAYAV GVADELDAAA AAADRMIDSL 430 440 450 460 470 480 VIDIETAADE SDKQDPGPGE GRWALHPEAM KLLAPPIEIS RDGRLLQHQA AIDEAHLVML 490 500 510 520 530 540 ADQGLVSQAA AADLLKHILD LQDEGFQSLE GRDAPRGVYL AYEAELAARA GPEKAGWLHL 550 560 570 580 590 600 ARSRNDLNAT ISLLVLREAA CSISQQIGIA QGALLQRVEE ASSLVAPLYS QYQIALPGSP 610 620 630 640 650 660 GHYLLGVFFA LGRERQRLHS LLEDIRNCPM GAGAGGGTSM PIDPLKTASL LGFEEPSFNS 670 680 690 700 710 720 LDAVASRDHH LHGLSIFASI STLLSRVAQD LQVWTTREFA LIDVPRNLAG GSSMLPQKKN 730 740 750 760 770 780 PFLLEHIKGS ASTVIGAYVS AATATCKAPF SNSIEVSNYG CSPLRLSEEA LQRALILTSL 790 800 810 820 830 840 IVKGMSFNVR SMRDHLEDGS SMTAIAAERM ASRGIPFREA HTQIGEIAQR LSQDDCATQR 850 860 870 880 890 900 RSELASQLAG VFPVSLEECR DALQFGGGPG KRSTDDQLSV AKTHFREMEL KCAEISERWA 910 920 HAEAHCKGRV KELIDKHRST CRSGVCG

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000013 Genomic DNA. Translation: BAB54609.1.
RefSeq	NP_085768.2. NC_002679.1.
3D structure databases
ProteinModelPortal	Q981V0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1231607.
GenomeReviews	Gene locus mll9226 in contig BA000013_GR.
KEGG	mlo:mll9226.
NMPDR	fig|266835.1.peg.6922.
PATRIC	22488320. VBIMesLot2464_7029.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG375485.
OMA	PIYSQYQ.
ProtClustDB	PRK02186.
Family and domain databases
HAMAP	MF_00006. Arg_succ_lyase. Fused. [Tree]
InterPro	IPR009049. Argininosuccinate_lyase. IPR011761. ATP-grasp. IPR003806. ATP-grasp_DUF201-type. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR005479. CarbamoylP_synth_lsu_ATP-bd. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR008948. L-Aspartase-like. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KO	K01755.
PANTHER	PTHR11444:SF3. argH. 1 hit.
Pfam	PF02655. ATP-grasp_3. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00867. CPSASE_2. 1 hit. Uncertain. PS00163. FUMARATE_LYASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLY2_RHILO

Accession

Primary (citable) accession number: Q981V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program