Reviewed, UniProtKB/Swiss-Prot Q981H7 (ALR3_RHILO)

Names and origin

Protein names

Recommended name:
    Alanine racemase 3
    EC=5.1.1.1

Gene names

Name:	alr3
Ordered Locus Names:	mlr9371

Encoded on

Plasmid pMLa

Organism

Rhizobium loti (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

381 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Alanine racemase 3 HAMAP MF_01201		PRO_0000114553
Sites
	Active site	41	1	Proton acceptor; specific for D-alanine By similarity
	Active site	256	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	41	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q981H7-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 04355B8341D254A3	FASTA	388	41,130
	10 20 30 40 50 60 MLTSVRPADV VLEIDLSAIQ ANFQTISALV GPQVRVAAVV KSDAYGLGLV KVAGALIDAG 70 80 90 100 110 120 CDLLFVGNLH EALLLRSSHI SAAVAVFCDE FARYGEHYRS NGLIPVVNNS VELDAICGAR 130 140 150 160 170 180 EPQAYFLNVE TGLSRLGLAF DDVRRRYLGG IFKRRPPSVV LSHLACSERA GDAMNLLQWN 190 200 210 220 230 240 RFRATSDLLK PTLLSLAASA GVWLGKRYHF DMVRVGSALY GLNSAGIRPN PLKPVVGVKA 250 260 270 280 290 300 KTLDARNVAR SEAVGYGATF RTGRASRLAI AGIGYKHGLP WACANKISVR FAGYSAPLVG 310 320 330 340 350 360 RVSMEYITID VTDVPEALCG PGTNVELLSD DFTVDDLAAS AGVHPQEVLT RLGVGCARQY 370 380 LDGSSASAGF PGNLTNAGPG HDPRAILG

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000013 Genomic DNA. Translation: BAB54978.1.
RefSeq	NP_085891.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1231478.
GenomeReviews	Gene locus mlr9371 in contig BA000013_GR.
KEGG	mlo:mlr9371.
NMPDR	fig|266835.1.peg.7045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q981H7.
OMA	KSKIMAV.
Enzyme and pathway databases
BRENDA	5.1.1.1. 3315.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR3_RHILO

Accession

Primary (citable) accession number: Q981H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)