Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Flap endonuclease 1

Gene

fen

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity). DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.By similarity1 Publication

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Enzyme regulationi

Heterotrimeric PCNA stimulates the nuclease activity without altering cleavage specificity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi27 – 271Magnesium 1UniRule annotation
Metal bindingi80 – 801Magnesium 1UniRule annotation
Metal bindingi152 – 1521Magnesium 1UniRule annotation
Metal bindingi154 – 1541Magnesium 1UniRule annotation
Metal bindingi173 – 1731Magnesium 2UniRule annotation
Metal bindingi175 – 1751Magnesium 2UniRule annotation
Metal bindingi236 – 2361Magnesium 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-168-MONOMER.
BRENDAi3.1.99.B1. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:fenUniRule annotation
Ordered Locus Names:SSO0179
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi341 – 3499Missing : No interaction with PCNA1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Flap endonuclease 1PRO_0000154065Add
BLAST

Interactioni

Subunit structurei

Interacts with PCNA via subunit PCNA1.2 Publications

Protein-protein interaction databases

STRINGi273057.SSO0179.

Structurei

Secondary structure

1
349
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 194Combined sources
Beta strandi22 – 276Combined sources
Helixi28 – 3710Combined sources
Beta strandi50 – 523Combined sources
Helixi56 – 7015Combined sources
Beta strandi73 – 797Combined sources
Helixi129 – 14214Combined sources
Beta strandi146 – 1483Combined sources
Helixi153 – 16210Combined sources
Beta strandi165 – 1706Combined sources
Beta strandi172 – 1743Combined sources
Helixi175 – 1784Combined sources
Beta strandi182 – 1887Combined sources
Beta strandi208 – 2114Combined sources
Helixi212 – 2198Combined sources
Helixi223 – 23311Combined sources
Beta strandi236 – 2383Combined sources
Helixi247 – 25610Combined sources
Helixi281 – 2866Combined sources
Helixi304 – 3107Combined sources
Turni311 – 3155Combined sources
Helixi319 – 34022Combined sources
Helixi345 – 3473Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IZOX-ray2.90A5-349[»]
ProteinModelPortaliQ980U8.
SMRiQ980U8. Positions 5-349.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ980U8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9898N-domainAdd
BLAST
Regioni116 – 258143I-domainAdd
BLAST
Regioni341 – 3499Interaction with PCNA

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04050. Archaea.
COG0258. LUCA.
HOGENOMiHOG000193852.
InParanoidiQ980U8.
KOiK04799.
OMAiGSQDYDS.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR019973. Flap_structure-sp_endonuc_arc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR03674. fen_arch. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q980U8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLADLVKDV KRELSFSELK GKRVSIDGYN ALYQFLAAIR QPDGTPLMDS
60 70 80 90 100
QGRVTSHLSG LFYRTINILE EGVIPIYVFD GKPPEQKSEE LERRRKAKEE
110 120 130 140 150
AERKLERAKS EGKIEELRKY SQAILRLSNI MVEESKKLLR AMGIPIVQAP
160 170 180 190 200
SEGEAEAAYL NKLGLSWAAA SQDYDAILFG AKRLVRNLTI TGKRKLPNKD
210 220 230 240 250
VYVEIKPELI ETEILLKKLG ITREQLIDIG ILIGTDYNPD GIRGIGPERA
260 270 280 290 300
LKIIKKYGKI EKAMEYGEIS KKDINFNIDE IRGLFLNPQV VKPEEALDLN
310 320 330 340
EPNGEDIINI LVYEHNFSEE RVKNGIERLT KAIKEAKGAS RQTGLDRWF
Length:349
Mass (Da):39,598
Last modified:February 12, 2003 - v2
Checksum:i7210395FD749C94C
GO

Sequence cautioni

The sequence AAK40525 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40525.1. Different initiation.
RefSeqiWP_009990408.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40525; AAK40525; SSO0179.
GeneIDi27426471.
KEGGisso:SSO0179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40525.1. Different initiation.
RefSeqiWP_009990408.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IZOX-ray2.90A5-349[»]
ProteinModelPortaliQ980U8.
SMRiQ980U8. Positions 5-349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO0179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40525; AAK40525; SSO0179.
GeneIDi27426471.
KEGGisso:SSO0179.

Phylogenomic databases

eggNOGiarCOG04050. Archaea.
COG0258. LUCA.
HOGENOMiHOG000193852.
InParanoidiQ980U8.
KOiK04799.
OMAiGSQDYDS.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-168-MONOMER.
BRENDAi3.1.99.B1. 6163.

Miscellaneous databases

EvolutionaryTraceiQ980U8.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR019973. Flap_structure-sp_endonuc_arc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR03674. fen_arch. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFEN_SULSO
AccessioniPrimary (citable) accession number: Q980U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: February 12, 2003
Last modified: September 7, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.