Q980U4 (HEM3_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable porphobilinogen deaminase
Short name=PBG
EC=2.5.1.61

Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase

Gene names

Name:	hemC
Ordered Locus Names:	SSO0183

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps. HAMAP MF_00260
Catalytic activity	4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP MF_00260
Cofactor	Binds 1 dipyrromethane group covalently By similarity. HAMAP MF_00260
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP MF_00260
Miscellaneous	The porphobilinogen subunits are added to the dipyrromethan group. HAMAP MF_00260
Sequence similarities	Belongs to the HMBS family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptidyl-pyrromethane cofactor linkage Inferred from electronic annotation. Source: InterPro porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydroxymethylbilane synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Probable porphobilinogen deaminase HAMAP MF_00260		PRO_0000143030
Amino acid modifications
	Modified residue	233	1	S-(dipyrrolylmethanemethyl)cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q980U4 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: BC7E398700151F49	FASTA	293	32,773
	10 20 30 40 50 60 MKILIAARGS KLSRVQVDMV GEKLRKIGIE YDVIDIKTKA DLFSTEPLSK LGKGVFEKEV 70 80 90 100 110 120 NEAVLEGKAD VAVHSMKDIL SEINPELEIF AVIERDPPYD ILIAEKNLHK LDPNTTIGTS 130 140 150 160 170 180 SIRRKNFIKY IRPEINTKDI RGNVDTRIRK YLSKEYDGLI LAEASIKRLN ISISYHRLNV 190 200 210 220 230 240 YDFTPEANQG IIVVLGRKKD EKVKEVFKEI THKDTLDEAL AERATISVVG GGCHSPIGVL 250 260 270 280 290 FRKEGKEFYG IASYSDGKKK ITVSISKAGD PYTIGSELGL LLKKEMKNEG IIP

References

[1]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006641 Genomic DNA. Translation: AAK40529.1.
PIR	B90159.
RefSeq	NP_341739.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q980U4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455339.
GenomeReviews	Gene locus SSO0183 in contig AE006641_GR.
KEGG	sso:SSO0183.
NMPDR	fig|273057.1.peg.162.
Phylogenomic databases
HOGENOM	HBG624842.
OMA	EKTILPC.
ProtClustDB	PRK00072.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0183-MONOMER.
Family and domain databases
HAMAP	MF_00260. Porphobil_deam. [Tree]
InterPro	IPR000860. 4pyrrol_synth_OHMeBilane_synth. IPR022419. Porphobilin_deaminase_cofac_BS. IPR022417. Porphobilin_deaminase_N. IPR022418. Porphobilinogen_deaminase_C. [Graphical view]
Gene3D	G3DSA:3.30.160.40. Porphobil_deam. 1 hit.
KO	K01749.
PANTHER	PTHR11557. Porphobil_deam. 1 hit.
Pfam	PF01379. Porphobil_deam. 1 hit. PF03900. Porphobil_deamC. 1 hit. [Graphical view]
PIRSF	PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTS	PR00151. PORPHBDMNASE.
SUPFAM	SSF54782. Porphobil_deam. 1 hit.
TIGRFAMs	TIGR00212. HemC. 1 hit.
PROSITE	PS00533. PORPHOBILINOGEN_DEAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM3_SULSO

Accession

Primary (citable) accession number: Q980U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2001
Last modified:	November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program